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Role for a region of helically unstable DNA within the Epstein-Barr virus latent cycle origin of DNA replication oriP in origin function.
Virology. 2004 Oct 25; 328(2):282-91.V

Abstract

The minimal replicator of the Epstein-Barr virus (EBV) latent cycle origin of DNA replication oriP is composed of two binding sites for the Epstein-Barr virus nuclear antigen-1 (EBNA-1) and flanking inverted repeats that bind the telomere repeat binding factor TRF2. Although not required for minimal replicator activity, additional binding sites for EBNA-1 and TRF2 and one or more auxiliary elements located to the right of the EBNA-1/TRF2 sites are required for the efficient replication of oriP plasmids. Another region of oriP that is predicted to be destabilized by DNA supercoiling is shown here to be an important functional component of oriP. The ability of DNA fragments of unrelated sequence and possessing supercoiled-induced DNA duplex destabilized (SIDD) structures, but not fragments characterized by helically stable DNA, to substitute for this component of oriP demonstrates a role for the SIDD region in the initiation of oriP-plasmid DNA replication.

Authors+Show Affiliations

Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15464848

Citation

Polonskaya, Zhanna, et al. "Role for a Region of Helically Unstable DNA Within the Epstein-Barr Virus Latent Cycle Origin of DNA Replication oriP in Origin Function." Virology, vol. 328, no. 2, 2004, pp. 282-91.
Polonskaya Z, Benham CJ, Hearing J. Role for a region of helically unstable DNA within the Epstein-Barr virus latent cycle origin of DNA replication oriP in origin function. Virology. 2004;328(2):282-91.
Polonskaya, Z., Benham, C. J., & Hearing, J. (2004). Role for a region of helically unstable DNA within the Epstein-Barr virus latent cycle origin of DNA replication oriP in origin function. Virology, 328(2), 282-91.
Polonskaya Z, Benham CJ, Hearing J. Role for a Region of Helically Unstable DNA Within the Epstein-Barr Virus Latent Cycle Origin of DNA Replication oriP in Origin Function. Virology. 2004 Oct 25;328(2):282-91. PubMed PMID: 15464848.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role for a region of helically unstable DNA within the Epstein-Barr virus latent cycle origin of DNA replication oriP in origin function. AU - Polonskaya,Zhanna, AU - Benham,Craig J, AU - Hearing,Janet, PY - 2004/05/07/received PY - 2004/05/27/revised PY - 2004/07/23/accepted PY - 2004/10/7/pubmed PY - 2004/12/16/medline PY - 2004/10/7/entrez SP - 282 EP - 91 JF - Virology JO - Virology VL - 328 IS - 2 N2 - The minimal replicator of the Epstein-Barr virus (EBV) latent cycle origin of DNA replication oriP is composed of two binding sites for the Epstein-Barr virus nuclear antigen-1 (EBNA-1) and flanking inverted repeats that bind the telomere repeat binding factor TRF2. Although not required for minimal replicator activity, additional binding sites for EBNA-1 and TRF2 and one or more auxiliary elements located to the right of the EBNA-1/TRF2 sites are required for the efficient replication of oriP plasmids. Another region of oriP that is predicted to be destabilized by DNA supercoiling is shown here to be an important functional component of oriP. The ability of DNA fragments of unrelated sequence and possessing supercoiled-induced DNA duplex destabilized (SIDD) structures, but not fragments characterized by helically stable DNA, to substitute for this component of oriP demonstrates a role for the SIDD region in the initiation of oriP-plasmid DNA replication. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/15464848/Role_for_a_region_of_helically_unstable_DNA_within_the_Epstein_Barr_virus_latent_cycle_origin_of_DNA_replication_oriP_in_origin_function_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(04)00499-4 DB - PRIME DP - Unbound Medicine ER -