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The structure of cell wall alpha-glucan from fission yeast.
Glycobiology. 2005 Mar; 15(3):245-57.G

Abstract

Morphology and structural integrity of fungal cells depend on cell wall polysaccharides. The chemical structure and biosynthesis of two types of these polysaccharides, chitin and (1-->3)-beta-glucan, have been studied extensively, whereas little is known about alpha-glucan. Here we describe the chemical structure of alpha-glucan isolated from wild-type and mutant cell walls of the fission yeast Schizosaccharomyces pombe. Wild-type alpha-glucan was found to consist of a single population of linear glucose polymers, approximately 260 residues in length. These glucose polymers were composed of two interconnected linear chains, each consisting of approximately 120 (1-->3)-linked alpha-d-glucose residues and some (1-->4)-linked alpha-D-glucose residues at the reducing end. By contrast, alpha-glucan of an alpha-glucan synthase mutant with an aberrant cell morphology and reduced alpha-glucan levels consisted of a single chain only. We propose that alpha-glucan biosynthesis involves an ordered series of events, whereby two alpha-glucan chains are coupled to create mature cell wall alpha-glucan. This mature form of cell wall alpha-glucan is essential for fission-yeast morphogenesis.

Authors+Show Affiliations

Grün CH
Bijvoet Center, Department of Bio-Organic Chemistry, Section of Glycoscience and Biocatalysis, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Hochstenbach F
No affiliation info available
Humbel BM
No affiliation info available
Verkleij AJ
No affiliation info available
Sietsma JH
No affiliation info available
Klis FM
No affiliation info available
Kamerling JP
No affiliation info available
Vliegenthart JF
No affiliation info available

MeSH

Carbohydrate SequenceCell WallGlucansGlucoseMagnetic Resonance SpectroscopyMicroscopy, Electron, TransmissionMolecular Sequence DataMutationSchizosaccharomyces

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15470229

Citation

Grün, Christian H., et al. "The Structure of Cell Wall Alpha-glucan From Fission Yeast." Glycobiology, vol. 15, no. 3, 2005, pp. 245-57.
Grün CH, Hochstenbach F, Humbel BM, et al. The structure of cell wall alpha-glucan from fission yeast. Glycobiology. 2005;15(3):245-57.
Grün, C. H., Hochstenbach, F., Humbel, B. M., Verkleij, A. J., Sietsma, J. H., Klis, F. M., Kamerling, J. P., & Vliegenthart, J. F. (2005). The structure of cell wall alpha-glucan from fission yeast. Glycobiology, 15(3), 245-57.
Grün CH, et al. The Structure of Cell Wall Alpha-glucan From Fission Yeast. Glycobiology. 2005;15(3):245-57. PubMed PMID: 15470229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The structure of cell wall alpha-glucan from fission yeast. AU - Grün,Christian H, AU - Hochstenbach,Frans, AU - Humbel,Bruno M, AU - Verkleij,Arie J, AU - Sietsma,J Hans, AU - Klis,Frans M, AU - Kamerling,Johannis P, AU - Vliegenthart,Johannes F G, Y1 - 2004/10/06/ PY - 2004/10/8/pubmed PY - 2005/8/16/medline PY - 2004/10/8/entrez SP - 245 EP - 57 JF - Glycobiology JO - Glycobiology VL - 15 IS - 3 N2 - Morphology and structural integrity of fungal cells depend on cell wall polysaccharides. The chemical structure and biosynthesis of two types of these polysaccharides, chitin and (1-->3)-beta-glucan, have been studied extensively, whereas little is known about alpha-glucan. Here we describe the chemical structure of alpha-glucan isolated from wild-type and mutant cell walls of the fission yeast Schizosaccharomyces pombe. Wild-type alpha-glucan was found to consist of a single population of linear glucose polymers, approximately 260 residues in length. These glucose polymers were composed of two interconnected linear chains, each consisting of approximately 120 (1-->3)-linked alpha-d-glucose residues and some (1-->4)-linked alpha-D-glucose residues at the reducing end. By contrast, alpha-glucan of an alpha-glucan synthase mutant with an aberrant cell morphology and reduced alpha-glucan levels consisted of a single chain only. We propose that alpha-glucan biosynthesis involves an ordered series of events, whereby two alpha-glucan chains are coupled to create mature cell wall alpha-glucan. This mature form of cell wall alpha-glucan is essential for fission-yeast morphogenesis. SN - 0959-6658 UR - https://www.unboundmedicine.com/medline/citation/15470229/The_structure_of_cell_wall_alpha_glucan_from_fission_yeast_ DB - PRIME DP - Unbound Medicine ER -