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Parameter oscillations in a very high gravity medium continuous ethanol fermentation and their attenuation on a multistage packed column bioreactor system.
Biotechnol Bioeng. 2004 Dec 05; 88(5):558-66.BB

Abstract

The quasi-steady-states, marked by small fluctuations of residual glucose, ethanol, and biomass concentrations, and sustainable oscillations marked by big fluctuations of these monitored fermentation parameters were observed during the continuous ethanol fermentation of Saccharomyces cerevisiae when very high gravity media were fed and correspondingly high ethanol concentrations reached. A high ethanol concentration was shown to be one of the main factors that incited these oscillations, although the residual glucose level affected the patterns of these oscillations to some extent. The lag response of S. cerevisiae to high ethanol stress that causes the shifts of morphology, viability loss, and death of yeast cells is assumed to be one of the probable mechanisms behind these oscillations. It was predicted that the longer the delay of this response was, the longer the oscillation periods would be, which was validated by the experimental data and the comparison with the oscillatory behaviors reported for the ethanologen bacterium, Zymomonas mobilis. Furthermore, three tubular bioreactors in series were arranged to follow a stirred tank bioreactor to attenuate these oscillations. However, exaggerated oscillations were observed for the residual glucose, ethanol, and biomass concentrations measured in the broth from these tubular bioreactors. After the tubular reactors were packed with Intalox ceramic saddle packing, these oscillations were effectively attenuated and quasi-steady-states were observed during which there were very small fluctuations of residual glucose, ethanol, and biomass within the entire experimental run.

Authors+Show Affiliations

Bai FW
Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. fwbai@dlut.edu.cn
Chen LJ
No affiliation info available
Anderson WA
No affiliation info available
Moo-Young M
No affiliation info available

MeSH

ApoptosisBiological ClocksBioreactorsCell Culture TechniquesCell ProliferationCell SizeEthanolFermentationGlucoseHypergravitySaccharomyces cerevisiae

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15470717

Citation

Bai, F W., et al. "Parameter Oscillations in a Very High Gravity Medium Continuous Ethanol Fermentation and Their Attenuation On a Multistage Packed Column Bioreactor System." Biotechnology and Bioengineering, vol. 88, no. 5, 2004, pp. 558-66.
Bai FW, Chen LJ, Anderson WA, et al. Parameter oscillations in a very high gravity medium continuous ethanol fermentation and their attenuation on a multistage packed column bioreactor system. Biotechnol Bioeng. 2004;88(5):558-66.
Bai, F. W., Chen, L. J., Anderson, W. A., & Moo-Young, M. (2004). Parameter oscillations in a very high gravity medium continuous ethanol fermentation and their attenuation on a multistage packed column bioreactor system. Biotechnology and Bioengineering, 88(5), 558-66.
Bai FW, et al. Parameter Oscillations in a Very High Gravity Medium Continuous Ethanol Fermentation and Their Attenuation On a Multistage Packed Column Bioreactor System. Biotechnol Bioeng. 2004 Dec 5;88(5):558-66. PubMed PMID: 15470717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parameter oscillations in a very high gravity medium continuous ethanol fermentation and their attenuation on a multistage packed column bioreactor system. AU - Bai,F W, AU - Chen,L J, AU - Anderson,W A, AU - Moo-Young,M, PY - 2004/10/8/pubmed PY - 2005/4/1/medline PY - 2004/10/8/entrez SP - 558 EP - 66 JF - Biotechnology and bioengineering JO - Biotechnol Bioeng VL - 88 IS - 5 N2 - The quasi-steady-states, marked by small fluctuations of residual glucose, ethanol, and biomass concentrations, and sustainable oscillations marked by big fluctuations of these monitored fermentation parameters were observed during the continuous ethanol fermentation of Saccharomyces cerevisiae when very high gravity media were fed and correspondingly high ethanol concentrations reached. A high ethanol concentration was shown to be one of the main factors that incited these oscillations, although the residual glucose level affected the patterns of these oscillations to some extent. The lag response of S. cerevisiae to high ethanol stress that causes the shifts of morphology, viability loss, and death of yeast cells is assumed to be one of the probable mechanisms behind these oscillations. It was predicted that the longer the delay of this response was, the longer the oscillation periods would be, which was validated by the experimental data and the comparison with the oscillatory behaviors reported for the ethanologen bacterium, Zymomonas mobilis. Furthermore, three tubular bioreactors in series were arranged to follow a stirred tank bioreactor to attenuate these oscillations. However, exaggerated oscillations were observed for the residual glucose, ethanol, and biomass concentrations measured in the broth from these tubular bioreactors. After the tubular reactors were packed with Intalox ceramic saddle packing, these oscillations were effectively attenuated and quasi-steady-states were observed during which there were very small fluctuations of residual glucose, ethanol, and biomass within the entire experimental run. SN - 0006-3592 UR - https://www.unboundmedicine.com/medline/citation/15470717/Parameter_oscillations_in_a_very_high_gravity_medium_continuous_ethanol_fermentation_and_their_attenuation_on_a_multistage_packed_column_bioreactor_system_ DB - PRIME DP - Unbound Medicine ER -