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Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum beta-lactamases and strains with extended-spectrum activity of the chromosomal beta-lactamase.
J Antimicrob Chemother. 2004 Nov; 54(5):881-8.JA

Abstract

OBJECTIVES

This study was conducted to analyse broad-spectrum cephalosporin-resistant Klebsiella oxytoca strains.

METHODS

The 57 isolates studied were recovered from clinical specimens (n=23) or from rectal swabs (n=34) during a 26-month period. Antibiotic susceptibility patterns were determined using standard agar diffusion and dilution methods including the synergy test between extended-spectrum cephalosporins and clavulanic acid. ERIC-2 PCR and pulsed-field gel electrophoresis (PFGE) methods were used to study the clonal relatedness of the strains. Plasmid-mediated and chromosomal beta-lactamases were characterized by mating and specific bla gene amplification and sequencing.

RESULTS

Four different antibiotic resistance patterns were identified whereas ERIC-2 PCR and PFGE revealed six main profiles. Extended-spectrum beta-lactamases (ESBLs) were found in 32 strains: TEM-7 (n=26), TEM-129 (n=1), TEM-3 (n=4), SHV-2 (n=1). The new TEM-type beta-lactamase, TEM-129, differed from TEM-7 by one mutation (Glu-104-->Lys). All TEM-7 or TEM-129 producers were genetically related. Twenty-five other strains with identical ERIC-2 PCR and PFGE profiles harboured a bla(OXY-2) gene different from the reference gene: 24 strains displayed one substitution (Ala-237-->Ser) in the KTG motif and one strain, highly resistant to ceftazidime, showed an additional substitution (Pro-167-->Ser).

CONCLUSIONS

The study demonstrated that the majority of strains (n=52) harbouring the OXY-2-type beta-lactamase corresponded to two clones. The first clone (n=27) corresponded to ESBL-producing strains. The second clone (n=25) displayed extended-spectrum activity of the chromosomal beta-lactamase.

Authors+Show Affiliations

UPRES n EA2392, Faculté de Médecine, UFR Saint-Antoine, Université Paris 6. dominique.decre@chusa.jussie.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15472005

Citation

Decré, Dominique, et al. "Outbreak of Multi-resistant Klebsiella Oxytoca Involving Strains With Extended-spectrum Beta-lactamases and Strains With Extended-spectrum Activity of the Chromosomal Beta-lactamase." The Journal of Antimicrobial Chemotherapy, vol. 54, no. 5, 2004, pp. 881-8.
Decré D, Burghoffer B, Gautier V, et al. Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum beta-lactamases and strains with extended-spectrum activity of the chromosomal beta-lactamase. J Antimicrob Chemother. 2004;54(5):881-8.
Decré, D., Burghoffer, B., Gautier, V., Petit, J. C., & Arlet, G. (2004). Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum beta-lactamases and strains with extended-spectrum activity of the chromosomal beta-lactamase. The Journal of Antimicrobial Chemotherapy, 54(5), 881-8.
Decré D, et al. Outbreak of Multi-resistant Klebsiella Oxytoca Involving Strains With Extended-spectrum Beta-lactamases and Strains With Extended-spectrum Activity of the Chromosomal Beta-lactamase. J Antimicrob Chemother. 2004;54(5):881-8. PubMed PMID: 15472005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum beta-lactamases and strains with extended-spectrum activity of the chromosomal beta-lactamase. AU - Decré,Dominique, AU - Burghoffer,Béatrice, AU - Gautier,Valérie, AU - Petit,Jean-Claude, AU - Arlet,Guillaume, Y1 - 2004/10/07/ PY - 2004/10/9/pubmed PY - 2005/4/1/medline PY - 2004/10/9/entrez SP - 881 EP - 8 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 54 IS - 5 N2 - OBJECTIVES: This study was conducted to analyse broad-spectrum cephalosporin-resistant Klebsiella oxytoca strains. METHODS: The 57 isolates studied were recovered from clinical specimens (n=23) or from rectal swabs (n=34) during a 26-month period. Antibiotic susceptibility patterns were determined using standard agar diffusion and dilution methods including the synergy test between extended-spectrum cephalosporins and clavulanic acid. ERIC-2 PCR and pulsed-field gel electrophoresis (PFGE) methods were used to study the clonal relatedness of the strains. Plasmid-mediated and chromosomal beta-lactamases were characterized by mating and specific bla gene amplification and sequencing. RESULTS: Four different antibiotic resistance patterns were identified whereas ERIC-2 PCR and PFGE revealed six main profiles. Extended-spectrum beta-lactamases (ESBLs) were found in 32 strains: TEM-7 (n=26), TEM-129 (n=1), TEM-3 (n=4), SHV-2 (n=1). The new TEM-type beta-lactamase, TEM-129, differed from TEM-7 by one mutation (Glu-104-->Lys). All TEM-7 or TEM-129 producers were genetically related. Twenty-five other strains with identical ERIC-2 PCR and PFGE profiles harboured a bla(OXY-2) gene different from the reference gene: 24 strains displayed one substitution (Ala-237-->Ser) in the KTG motif and one strain, highly resistant to ceftazidime, showed an additional substitution (Pro-167-->Ser). CONCLUSIONS: The study demonstrated that the majority of strains (n=52) harbouring the OXY-2-type beta-lactamase corresponded to two clones. The first clone (n=27) corresponded to ESBL-producing strains. The second clone (n=25) displayed extended-spectrum activity of the chromosomal beta-lactamase. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/15472005/Outbreak_of_multi_resistant_Klebsiella_oxytoca_involving_strains_with_extended_spectrum_beta_lactamases_and_strains_with_extended_spectrum_activity_of_the_chromosomal_beta_lactamase_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkh440 DB - PRIME DP - Unbound Medicine ER -