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Microemulsion electrokinetic chromatography for the analysis of acrylamide in food.
Electrophoresis. 2004 Oct; 25(18-19):3257-62.E

Abstract

The influence of microemulsion electrokinetic chromatography (MEEKC) operating conditions, such as the type of water-immiscible alcohol, aqueous phosphate buffer concentration, pH, as well as the addition of methanol and 2-propanol, on acrylamide migration has been studied. These parameters have been optimized taking into account the presence of matrix signals, in order to avoid the interference of these peaks in acrylamide determination. The best separations were achieved using a microemulsion consisting of 0.8% m/v n-amyl alcohol, 3.3% m/v sodium dodecyl sulfate (SDS), 6.6% m/v 1-butanol, and 89.3% m/v 40 mM phosphate buffer at pH 6.5 working at 15 kV in uncoated silica capillaries. Linear calibration curves over the range studied (1.25-125 microg x mL(-1)), the detection limit (0.70 microg x mL(-1)), and both run-to-run (up to 3.4% for concentration and 1.6% for time values) and day-to-day precision (lower than 11.6% for concentration) have been established. Finally, the applicability of the MEEKC method developed has been demonstrated by analyzing levels of acrylamide present in samples of home-made French fries.

Authors+Show Affiliations

Bermudo E
Department of Analytical Chemistry, University of Barcelona, Barcelona, Spain.
Ruiz-Calero V
No affiliation info available
Puignou L
No affiliation info available
Galceran MT
No affiliation info available

MeSH

AcrylamideCalibrationChromatography, Micellar Electrokinetic CapillaryEmulsionsFood AnalysisReproducibility of Results

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15472956

Citation

Bermudo, Elisabet, et al. "Microemulsion Electrokinetic Chromatography for the Analysis of Acrylamide in Food." Electrophoresis, vol. 25, no. 18-19, 2004, pp. 3257-62.
Bermudo E, Ruiz-Calero V, Puignou L, et al. Microemulsion electrokinetic chromatography for the analysis of acrylamide in food. Electrophoresis. 2004;25(18-19):3257-62.
Bermudo, E., Ruiz-Calero, V., Puignou, L., & Galceran, M. T. (2004). Microemulsion electrokinetic chromatography for the analysis of acrylamide in food. Electrophoresis, 25(18-19), 3257-62.
Bermudo E, et al. Microemulsion Electrokinetic Chromatography for the Analysis of Acrylamide in Food. Electrophoresis. 2004;25(18-19):3257-62. PubMed PMID: 15472956.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microemulsion electrokinetic chromatography for the analysis of acrylamide in food. AU - Bermudo,Elisabet, AU - Ruiz-Calero,Victoria, AU - Puignou,Lluis, AU - Galceran,Maria T, PY - 2004/10/9/pubmed PY - 2005/3/23/medline PY - 2004/10/9/entrez SP - 3257 EP - 62 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 18-19 N2 - The influence of microemulsion electrokinetic chromatography (MEEKC) operating conditions, such as the type of water-immiscible alcohol, aqueous phosphate buffer concentration, pH, as well as the addition of methanol and 2-propanol, on acrylamide migration has been studied. These parameters have been optimized taking into account the presence of matrix signals, in order to avoid the interference of these peaks in acrylamide determination. The best separations were achieved using a microemulsion consisting of 0.8% m/v n-amyl alcohol, 3.3% m/v sodium dodecyl sulfate (SDS), 6.6% m/v 1-butanol, and 89.3% m/v 40 mM phosphate buffer at pH 6.5 working at 15 kV in uncoated silica capillaries. Linear calibration curves over the range studied (1.25-125 microg x mL(-1)), the detection limit (0.70 microg x mL(-1)), and both run-to-run (up to 3.4% for concentration and 1.6% for time values) and day-to-day precision (lower than 11.6% for concentration) have been established. Finally, the applicability of the MEEKC method developed has been demonstrated by analyzing levels of acrylamide present in samples of home-made French fries. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15472956/Microemulsion_electrokinetic_chromatography_for_the_analysis_of_acrylamide_in_food_ DB - PRIME DP - Unbound Medicine ER -