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Ethanolic leaf extract of neem (Azadirachta indica) inhibits buccal pouch carcinogenesis in hamsters.
Cell Biochem Funct. 2005 Jul-Aug; 23(4):229-38.CB

Abstract

We evaluated the chemopreventive effects of ethanolic neem leaf extract in the initiation and post-initiation phases of 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. The frequency of bone marrow micronuclei as well as the concentrations of lipid peroxides, ratio of reduced to oxidized glutathione (GSH/GSSG), and the activities of the GSH-dependent enzymes glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in the buccal pouch, liver and erythrocytes were used as biomarkers of chemoprevention. All the hamsters painted with DMBA alone for 14 weeks developed buccal pouch carcinomas that showed diminished lipid peroxidation and enhanced antioxidant status associated with increased frequencies of bone marrow micronuclei. In the liver and erythrocytes of tumour-bearing animals, enhanced lipid peroxidation was accompanied by compromised antioxidant defences. Administration of ethanolic neem leaf extract effectively suppressed DMBA-induced HBP carcinogenesis as revealed by the absence of tumours in the initiation phase and reduced tumour incidence in the post-initiation phase. In addition, ethanolic neem leaf extract modulated lipid peroxidation and enhanced antioxidant status in the pouch, liver and erythrocytes and reduced the incidence of bone marrow micronuclei. The results of the present study, demonstrate that ethanolic neem leaf extract inhibits the development of DMBA-induced HBP tumours by protecting against oxidative stress.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Science, Annamalai University, Tamil Nadu, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15473007

Citation

Subapriya, R, et al. "Ethanolic Leaf Extract of Neem (Azadirachta Indica) Inhibits Buccal Pouch Carcinogenesis in Hamsters." Cell Biochemistry and Function, vol. 23, no. 4, 2005, pp. 229-38.
Subapriya R, Bhuvaneswari V, Ramesh V, et al. Ethanolic leaf extract of neem (Azadirachta indica) inhibits buccal pouch carcinogenesis in hamsters. Cell Biochem Funct. 2005;23(4):229-38.
Subapriya, R., Bhuvaneswari, V., Ramesh, V., & Nagini, S. (2005). Ethanolic leaf extract of neem (Azadirachta indica) inhibits buccal pouch carcinogenesis in hamsters. Cell Biochemistry and Function, 23(4), 229-38.
Subapriya R, et al. Ethanolic Leaf Extract of Neem (Azadirachta Indica) Inhibits Buccal Pouch Carcinogenesis in Hamsters. Cell Biochem Funct. 2005 Jul-Aug;23(4):229-38. PubMed PMID: 15473007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethanolic leaf extract of neem (Azadirachta indica) inhibits buccal pouch carcinogenesis in hamsters. AU - Subapriya,R, AU - Bhuvaneswari,V, AU - Ramesh,V, AU - Nagini,S, PY - 2004/10/9/pubmed PY - 2005/9/16/medline PY - 2004/10/9/entrez SP - 229 EP - 38 JF - Cell biochemistry and function JO - Cell Biochem Funct VL - 23 IS - 4 N2 - We evaluated the chemopreventive effects of ethanolic neem leaf extract in the initiation and post-initiation phases of 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. The frequency of bone marrow micronuclei as well as the concentrations of lipid peroxides, ratio of reduced to oxidized glutathione (GSH/GSSG), and the activities of the GSH-dependent enzymes glutathione peroxidase (GPx) and glutathione-S-transferase (GST) in the buccal pouch, liver and erythrocytes were used as biomarkers of chemoprevention. All the hamsters painted with DMBA alone for 14 weeks developed buccal pouch carcinomas that showed diminished lipid peroxidation and enhanced antioxidant status associated with increased frequencies of bone marrow micronuclei. In the liver and erythrocytes of tumour-bearing animals, enhanced lipid peroxidation was accompanied by compromised antioxidant defences. Administration of ethanolic neem leaf extract effectively suppressed DMBA-induced HBP carcinogenesis as revealed by the absence of tumours in the initiation phase and reduced tumour incidence in the post-initiation phase. In addition, ethanolic neem leaf extract modulated lipid peroxidation and enhanced antioxidant status in the pouch, liver and erythrocytes and reduced the incidence of bone marrow micronuclei. The results of the present study, demonstrate that ethanolic neem leaf extract inhibits the development of DMBA-induced HBP tumours by protecting against oxidative stress. SN - 0263-6484 UR - https://www.unboundmedicine.com/medline/citation/15473007/Ethanolic_leaf_extract_of_neem__Azadirachta_indica__inhibits_buccal_pouch_carcinogenesis_in_hamsters_ L2 - https://doi.org/10.1002/cbf.1143 DB - PRIME DP - Unbound Medicine ER -