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[Expression of matrix metalloproteinases-9,2,7,and tissue inhibitor of metalloproteinases-1,2,3 mRNA in ovarian tumors and their clinical significance].
Ai Zheng. 2004 Oct; 23(10):1194-8.AZ

Abstract

BACKGROUND & OBJECTIVE

Matrix metalloproteinases (MMPs)play an important role in cancer cell invasion,and metastasis by degrading the extracellular matrix (ECM). The activities of MMPs are regulated by tissue inhibitor of metalloproteinases (TIMPs). This study was to detect mRNA expression of MMP-9, 2, 7, and TIMP-1, 2, 3 in ovarian tumor tissues,and explore their correlations with clinicopathologic characteristics,and prognosis of patients with ovarian cancer.

METHODS

Reverse transcriptase-polymerase chain reaction (RT-PCR)was used to assay the positive rates,and half-quantity values of MMP-9, 2, 7, and TIMP-1, 2, 3 mRNA in 48 cases of malignant ovarian tumor tissues, 21 cases of benign ovarian tumor tissues, and 22 cases of normal ovarian tissues.

RESULTS

The positive rate of MMP-9 mRNA, and the half-quantity values of MMP-9,MMP-2 were significantly higher in malignant tumor tissues than those in normal ovarian tissues (P< 0.05). The positive rates and half-quantity values of TIMP-2,MMP-7,TIMP-3 mRNA were significantly higher in malignant and benign ovarian tumor tissues than those in normal ovarian tissues (P< 0.05). The ratio of MMP-9/TIMP-1 was higher in malignant ovarian tumor tissues(0.91+/-0.67) than that in normal ovarian tissues (0.14+/-0.33)(P< 0.05). The expression level of MMP-9 mRNA was higher in ovarian cancer tissues of stage III-IV(1.13+/-0.66) than those of stage I-II(0.60+/-0.54)(P< 0.05). The mean survival time of MMP-9 positive patients was (43.00+/-17.12) months,and cumulate survival rate was 47.37%,significantly lower than that of MMP-9 negative patients (100%).

CONCLUSIONS

MMP-9, MMP-2, MMP-7, TIMP-2, TIMP-3 are over-expressed in ovarian malignant tissues. The over-expression of MMP-9, and the imbalance between MMP-9 and TIMP-1 play important roles in the progress of advanced ovarian cancer. MMP-9 may be a useful prognostic marker for patients with advanced ovarian cancer.

Authors+Show Affiliations

Department of Gynecology Oncology, Affiliated Tumor Hospital, Guangxi Medicial University, Nanning,Guangxi,530021, P.R.China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15473934

Citation

Hu, Xiao-Xia, et al. "[Expression of Matrix Metalloproteinases-9,2,7,and Tissue Inhibitor of Metalloproteinases-1,2,3 mRNA in Ovarian Tumors and Their Clinical Significance]." Ai Zheng = Aizheng = Chinese Journal of Cancer, vol. 23, no. 10, 2004, pp. 1194-8.
Hu XX, Li L, Li DR, et al. [Expression of matrix metalloproteinases-9,2,7,and tissue inhibitor of metalloproteinases-1,2,3 mRNA in ovarian tumors and their clinical significance]. Ai Zheng. 2004;23(10):1194-8.
Hu, X. X., Li, L., Li, D. R., Zhang, W., Cheng, X. Q., Zhang, J. Q., & Tang, B. J. (2004). [Expression of matrix metalloproteinases-9,2,7,and tissue inhibitor of metalloproteinases-1,2,3 mRNA in ovarian tumors and their clinical significance]. Ai Zheng = Aizheng = Chinese Journal of Cancer, 23(10), 1194-8.
Hu XX, et al. [Expression of Matrix Metalloproteinases-9,2,7,and Tissue Inhibitor of Metalloproteinases-1,2,3 mRNA in Ovarian Tumors and Their Clinical Significance]. Ai Zheng. 2004;23(10):1194-8. PubMed PMID: 15473934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of matrix metalloproteinases-9,2,7,and tissue inhibitor of metalloproteinases-1,2,3 mRNA in ovarian tumors and their clinical significance]. AU - Hu,Xiao-Xia, AU - Li,Li, AU - Li,Dan-Rong, AU - Zhang,Wei, AU - Cheng,Xin-Qiu, AU - Zhang,Jie-Qing, AU - Tang,Bu-Jian, PY - 2004/10/12/pubmed PY - 2005/8/16/medline PY - 2004/10/12/entrez SP - 1194 EP - 8 JF - Ai zheng = Aizheng = Chinese journal of cancer JO - Ai Zheng VL - 23 IS - 10 N2 - BACKGROUND & OBJECTIVE: Matrix metalloproteinases (MMPs)play an important role in cancer cell invasion,and metastasis by degrading the extracellular matrix (ECM). The activities of MMPs are regulated by tissue inhibitor of metalloproteinases (TIMPs). This study was to detect mRNA expression of MMP-9, 2, 7, and TIMP-1, 2, 3 in ovarian tumor tissues,and explore their correlations with clinicopathologic characteristics,and prognosis of patients with ovarian cancer. METHODS: Reverse transcriptase-polymerase chain reaction (RT-PCR)was used to assay the positive rates,and half-quantity values of MMP-9, 2, 7, and TIMP-1, 2, 3 mRNA in 48 cases of malignant ovarian tumor tissues, 21 cases of benign ovarian tumor tissues, and 22 cases of normal ovarian tissues. RESULTS: The positive rate of MMP-9 mRNA, and the half-quantity values of MMP-9,MMP-2 were significantly higher in malignant tumor tissues than those in normal ovarian tissues (P< 0.05). The positive rates and half-quantity values of TIMP-2,MMP-7,TIMP-3 mRNA were significantly higher in malignant and benign ovarian tumor tissues than those in normal ovarian tissues (P< 0.05). The ratio of MMP-9/TIMP-1 was higher in malignant ovarian tumor tissues(0.91+/-0.67) than that in normal ovarian tissues (0.14+/-0.33)(P< 0.05). The expression level of MMP-9 mRNA was higher in ovarian cancer tissues of stage III-IV(1.13+/-0.66) than those of stage I-II(0.60+/-0.54)(P< 0.05). The mean survival time of MMP-9 positive patients was (43.00+/-17.12) months,and cumulate survival rate was 47.37%,significantly lower than that of MMP-9 negative patients (100%). CONCLUSIONS: MMP-9, MMP-2, MMP-7, TIMP-2, TIMP-3 are over-expressed in ovarian malignant tissues. The over-expression of MMP-9, and the imbalance between MMP-9 and TIMP-1 play important roles in the progress of advanced ovarian cancer. MMP-9 may be a useful prognostic marker for patients with advanced ovarian cancer. UR - https://www.unboundmedicine.com/medline/citation/15473934/[Expression_of_matrix_metalloproteinases_927and_tissue_inhibitor_of_metalloproteinases_123_mRNA_in_ovarian_tumors_and_their_clinical_significance]_ L2 - http://www.cancercommun.com/fulltextnew.asp?y=2004&amp;m=10&amp;ym=1194 DB - PRIME DP - Unbound Medicine ER -