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Production of macrophage inflammatory protein-3alpha in human follicular fluid and cultured granulosa cells.
Fertil Steril. 2004 Oct; 82 Suppl 3:1206-11.FS

Abstract

OBJECTIVE

To investigate the role of macrophage inflammatory protein (MIP)-3alpha in human ovulation.

DESIGN

Study of the levels of MIP-3alpha in serum and follicular fluid. The effects of interleukin (IL)-1alpha, IL-1 receptor antagonist (RA), and tumor necrosis factor (TNF)-alpha on the secretion of MIP-3alpha by primary cultured granulosa-lutein cells and an immortalized granulosa cell line (GC1a) were investigated.

SETTING

Research laboratory at a university medical school.

PATIENT(S)

Forty-six patients with sterility undergoing in vitro fertilization and embryo transfer (i.v.f.-ET).

INTERVENTION(S)

Follicular fluid was obtained from study participants, and granulosa-lutein cells and GC1a were incubated with IL-1alpha, IL-1RA, or TNF-alpha for 4 to 32 hours.

MAIN OUTCOME MEASURE(S)

The concentration of MIP-3alpha in human follicular fluid was measured and correlated with oocyte maturation. We also cultured granulosa cells and examined the regulation of MIP-3alpha production. The concentrations of MIP-3alpha in the serum, follicular fluid, and culture medium were measured using enzyme-linked immunoabsorbent assay.

RESULT(S)

Concentrations of MIP-3alpha were significantly higher in the follicular fluid, but it was not detected in the serum. Concentrations of MIP-3alpha were statistically significantly higher in the follicular fluid containing mature oocytes than in follicular fluid containing immature oocytes. The production of MIP-3alpha was markedly increased over the basal level after treatment with IL-1alpha and TNF-alpha in a dose-dependent manner. The stimulatory effect of IL-1alpha was inhibited by IL-1RA.

CONCLUSION(S)

Our data suggest that MIP-3alpha was present in follicular fluid and correlated with oocyte maturation, and was regulated by IL-1alpha and TNF-alpha. Thus, MIP-3alpha may play an important role in the human preovulatory process.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. kawayas@med.oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15474097

Citation

Kawano, Yasushi, et al. "Production of Macrophage Inflammatory Protein-3alpha in Human Follicular Fluid and Cultured Granulosa Cells." Fertility and Sterility, vol. 82 Suppl 3, 2004, pp. 1206-11.
Kawano Y, Fukuda J, Nasu K, et al. Production of macrophage inflammatory protein-3alpha in human follicular fluid and cultured granulosa cells. Fertil Steril. 2004;82 Suppl 3:1206-11.
Kawano, Y., Fukuda, J., Nasu, K., Nishida, M., Narahara, H., & Miyakawa, I. (2004). Production of macrophage inflammatory protein-3alpha in human follicular fluid and cultured granulosa cells. Fertility and Sterility, 82 Suppl 3, 1206-11.
Kawano Y, et al. Production of Macrophage Inflammatory Protein-3alpha in Human Follicular Fluid and Cultured Granulosa Cells. Fertil Steril. 2004;82 Suppl 3:1206-11. PubMed PMID: 15474097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of macrophage inflammatory protein-3alpha in human follicular fluid and cultured granulosa cells. AU - Kawano,Yasushi, AU - Fukuda,Junichiro, AU - Nasu,Kaei, AU - Nishida,Masakazu, AU - Narahara,Hisashi, AU - Miyakawa,Isao, PY - 2004/02/15/received PY - 2004/05/17/revised PY - 2004/05/17/accepted PY - 2004/10/12/pubmed PY - 2004/12/16/medline PY - 2004/10/12/entrez SP - 1206 EP - 11 JF - Fertility and sterility JO - Fertil Steril VL - 82 Suppl 3 N2 - OBJECTIVE: To investigate the role of macrophage inflammatory protein (MIP)-3alpha in human ovulation. DESIGN: Study of the levels of MIP-3alpha in serum and follicular fluid. The effects of interleukin (IL)-1alpha, IL-1 receptor antagonist (RA), and tumor necrosis factor (TNF)-alpha on the secretion of MIP-3alpha by primary cultured granulosa-lutein cells and an immortalized granulosa cell line (GC1a) were investigated. SETTING: Research laboratory at a university medical school. PATIENT(S): Forty-six patients with sterility undergoing in vitro fertilization and embryo transfer (i.v.f.-ET). INTERVENTION(S): Follicular fluid was obtained from study participants, and granulosa-lutein cells and GC1a were incubated with IL-1alpha, IL-1RA, or TNF-alpha for 4 to 32 hours. MAIN OUTCOME MEASURE(S): The concentration of MIP-3alpha in human follicular fluid was measured and correlated with oocyte maturation. We also cultured granulosa cells and examined the regulation of MIP-3alpha production. The concentrations of MIP-3alpha in the serum, follicular fluid, and culture medium were measured using enzyme-linked immunoabsorbent assay. RESULT(S): Concentrations of MIP-3alpha were significantly higher in the follicular fluid, but it was not detected in the serum. Concentrations of MIP-3alpha were statistically significantly higher in the follicular fluid containing mature oocytes than in follicular fluid containing immature oocytes. The production of MIP-3alpha was markedly increased over the basal level after treatment with IL-1alpha and TNF-alpha in a dose-dependent manner. The stimulatory effect of IL-1alpha was inhibited by IL-1RA. CONCLUSION(S): Our data suggest that MIP-3alpha was present in follicular fluid and correlated with oocyte maturation, and was regulated by IL-1alpha and TNF-alpha. Thus, MIP-3alpha may play an important role in the human preovulatory process. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/15474097/Production_of_macrophage_inflammatory_protein_3alpha_in_human_follicular_fluid_and_cultured_granulosa_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(04)01267-1 DB - PRIME DP - Unbound Medicine ER -