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A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors: analysis of 100 samples of pancreatic juice from consecutive patients.
Cancer. 2004 Nov 15; 101(10):2309-17.C

Abstract

BACKGROUND

Early detection of pancreatic carcinoma is difficult even with current diagnostic tools. Novel biomarkers and detection techniques are urgently needed. Telomerase activity is a promising diagnostic marker. However, the conventional telomeric repeat amplification protocol (TRAP) assay is not suitable for clinical application because of its complexity, time-consuming nature, and the effects of polymerase chain reaction (PCR) inhibitors in samples leading to difficulties in quantification.

METHODS

The authors used a hybridization protection assay in combination with TRAP (TRAP/HPA) to investigate the effects of PCR inhibitors in pancreatic juice on quantification of telomerase activity. They analyzed 117 consecutive samples of pancreatic juice to determine the feasibility of TRAP/HPA for diagnosis of pancreatic carcinoma.

RESULTS

The authors found that TRAP/HPA was 1000-fold more sensitive than the conventional TRAP assay, and that the effects of PCR inhibitors could be avoided by diluting samples. In a large analysis of pancreatic juice samples with TRAP/HPA, 17 samples were excluded from the final analysis because of insufficient follow-up periods or inadequate treatment of the samples. Relative telomerase activity (RTA) in samples from patients with pancreatic carcinoma was significantly higher in comparison to samples from patients with pancreatitis and 13 (61.9%) of 21 samples from patients with pancreatic carcinoma showed high RTA (> 4 U). Meanwhile, high RTAs were observed in 4 of 35 (11.4%) samples from patients with intraductal papillary mucinous tumor and in 1 of 40 samples (2.5%) fom patients without malignant disease.

CONCLUSIONS

TRAP/HPA accurately evaluated weak telomerase activity in pancreatic juice samples without the problem due to PCR inhibitors. This large analysis of nonselected pancreatic juice samples suggested that TRAP/HPA is a promising approach for the diagnosis of pancreatic carcinoma.

Authors+Show Affiliations

Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15476274

Citation

Ohuchida, Kenoki, et al. "A Highly Sensitive and Quantitative Telomerase Activity Assay With Pancreatic Juice Is Useful for Diagnosis of Pancreatic Carcinoma Without Problems Due to Polymerase Chain Reaction Inhibitors: Analysis of 100 Samples of Pancreatic Juice From Consecutive Patients." Cancer, vol. 101, no. 10, 2004, pp. 2309-17.
Ohuchida K, Mizumoto K, Ishikawa N, et al. A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors: analysis of 100 samples of pancreatic juice from consecutive patients. Cancer. 2004;101(10):2309-17.
Ohuchida, K., Mizumoto, K., Ishikawa, N., Sato, N., Nagai, E., Yamaguchi, K., Takaishi, H., Ide, T., & Tanaka, M. (2004). A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors: analysis of 100 samples of pancreatic juice from consecutive patients. Cancer, 101(10), 2309-17.
Ohuchida K, et al. A Highly Sensitive and Quantitative Telomerase Activity Assay With Pancreatic Juice Is Useful for Diagnosis of Pancreatic Carcinoma Without Problems Due to Polymerase Chain Reaction Inhibitors: Analysis of 100 Samples of Pancreatic Juice From Consecutive Patients. Cancer. 2004 Nov 15;101(10):2309-17. PubMed PMID: 15476274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A highly sensitive and quantitative telomerase activity assay with pancreatic juice is useful for diagnosis of pancreatic carcinoma without problems due to polymerase chain reaction inhibitors: analysis of 100 samples of pancreatic juice from consecutive patients. AU - Ohuchida,Kenoki, AU - Mizumoto,Kazuhiro, AU - Ishikawa,Nami, AU - Sato,Norihiro, AU - Nagai,Eishi, AU - Yamaguchi,Koji, AU - Takaishi,Hideki, AU - Ide,Toshinori, AU - Tanaka,Masao, PY - 2004/10/12/pubmed PY - 2004/12/23/medline PY - 2004/10/12/entrez SP - 2309 EP - 17 JF - Cancer JO - Cancer VL - 101 IS - 10 N2 - BACKGROUND: Early detection of pancreatic carcinoma is difficult even with current diagnostic tools. Novel biomarkers and detection techniques are urgently needed. Telomerase activity is a promising diagnostic marker. However, the conventional telomeric repeat amplification protocol (TRAP) assay is not suitable for clinical application because of its complexity, time-consuming nature, and the effects of polymerase chain reaction (PCR) inhibitors in samples leading to difficulties in quantification. METHODS: The authors used a hybridization protection assay in combination with TRAP (TRAP/HPA) to investigate the effects of PCR inhibitors in pancreatic juice on quantification of telomerase activity. They analyzed 117 consecutive samples of pancreatic juice to determine the feasibility of TRAP/HPA for diagnosis of pancreatic carcinoma. RESULTS: The authors found that TRAP/HPA was 1000-fold more sensitive than the conventional TRAP assay, and that the effects of PCR inhibitors could be avoided by diluting samples. In a large analysis of pancreatic juice samples with TRAP/HPA, 17 samples were excluded from the final analysis because of insufficient follow-up periods or inadequate treatment of the samples. Relative telomerase activity (RTA) in samples from patients with pancreatic carcinoma was significantly higher in comparison to samples from patients with pancreatitis and 13 (61.9%) of 21 samples from patients with pancreatic carcinoma showed high RTA (> 4 U). Meanwhile, high RTAs were observed in 4 of 35 (11.4%) samples from patients with intraductal papillary mucinous tumor and in 1 of 40 samples (2.5%) fom patients without malignant disease. CONCLUSIONS: TRAP/HPA accurately evaluated weak telomerase activity in pancreatic juice samples without the problem due to PCR inhibitors. This large analysis of nonselected pancreatic juice samples suggested that TRAP/HPA is a promising approach for the diagnosis of pancreatic carcinoma. SN - 0008-543X UR - https://www.unboundmedicine.com/medline/citation/15476274/A_highly_sensitive_and_quantitative_telomerase_activity_assay_with_pancreatic_juice_is_useful_for_diagnosis_of_pancreatic_carcinoma_without_problems_due_to_polymerase_chain_reaction_inhibitors:_analysis_of_100_samples_of_pancreatic_juice_from_consecutive_patients_ L2 - https://doi.org/10.1002/cncr.20649 DB - PRIME DP - Unbound Medicine ER -