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Potent antioxidative and antigenotoxic activity in aqueous extract of Japanese rice bran--association with peroxidase activity.
Phytother Res. 2004 Aug; 18(8):628-33.PR

Abstract

To estimate the preventive potential of Japanese rice bran (Oryza sativa japonica) against the oxygen radical-related chronic diseases such as cardio-vascular diseases and cancer, antioxidative and antigenotoxic activities of the rice bran extracts were analyzed by using assay systems for lipid peroxidation and genotoxin-induced umu gene expression. When effects of the rice bran extracts under different extraction conditions on hydroperoxide generation from auto-oxidized linoleic acid were examined using aluminum chloride method, the water extract showed strong antioxidant activity, but the methanol and acetone extracts did not exhibit significant activity. The water extract of rice bran was divided into the ethanol-precipitable (EP) and supernatant fractions, and EP fraction showed the dominant antioxidant activity, but the supernatant fraction did not exhibit significant antioxidant activity. When the effect of EP fraction on umu C gene expression in SOS response associated with DNA damage in Salmonella typhimurium (TA 1535/pSK 1002) induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was analyzed, it showed a dose-dependent suppressive activity against Trp-P-1-induced umu C gene expression. The bio-chemical analysis of EP fraction indicates that the major antioxidative and antigenotoxic activity of EP fraction is associated with a proteinous component with the molecular weight of more than 30 KDa. As a possible active principle for the antioxidative and antigenotoxic activity in EP fraction, the strong activity of an oxygen radical-scavenging enzyme, peroxidase was detected, and the purified horseradish peroxidase also caused the similar antioxidative and antigenotoxic activities. The significance of this finding is discussed from the viewpoint of the preventive role of rice bran against oxygen radical-related chronic diseases.

Authors+Show Affiliations

Department of Food and Nutrition, Hiroshima Jogakuin University, Ushita-Higashi, Higashi-Ku, Hiroshima 732-0063, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15476307

Citation

Higashi-Okai, Kiyoka, et al. "Potent Antioxidative and Antigenotoxic Activity in Aqueous Extract of Japanese Rice Bran--association With Peroxidase Activity." Phytotherapy Research : PTR, vol. 18, no. 8, 2004, pp. 628-33.
Higashi-Okai K, Kanbara K, Amano K, et al. Potent antioxidative and antigenotoxic activity in aqueous extract of Japanese rice bran--association with peroxidase activity. Phytother Res. 2004;18(8):628-33.
Higashi-Okai, K., Kanbara, K., Amano, K., Hagiwara, A., Sugita, C., Matsumoto, N., & Okai, Y. (2004). Potent antioxidative and antigenotoxic activity in aqueous extract of Japanese rice bran--association with peroxidase activity. Phytotherapy Research : PTR, 18(8), 628-33.
Higashi-Okai K, et al. Potent Antioxidative and Antigenotoxic Activity in Aqueous Extract of Japanese Rice Bran--association With Peroxidase Activity. Phytother Res. 2004;18(8):628-33. PubMed PMID: 15476307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potent antioxidative and antigenotoxic activity in aqueous extract of Japanese rice bran--association with peroxidase activity. AU - Higashi-Okai,Kiyoka, AU - Kanbara,Keiko, AU - Amano,Kanako, AU - Hagiwara,Akiko, AU - Sugita,Chie, AU - Matsumoto,Norie, AU - Okai,Yasuji, PY - 2004/10/12/pubmed PY - 2004/12/16/medline PY - 2004/10/12/entrez SP - 628 EP - 33 JF - Phytotherapy research : PTR JO - Phytother Res VL - 18 IS - 8 N2 - To estimate the preventive potential of Japanese rice bran (Oryza sativa japonica) against the oxygen radical-related chronic diseases such as cardio-vascular diseases and cancer, antioxidative and antigenotoxic activities of the rice bran extracts were analyzed by using assay systems for lipid peroxidation and genotoxin-induced umu gene expression. When effects of the rice bran extracts under different extraction conditions on hydroperoxide generation from auto-oxidized linoleic acid were examined using aluminum chloride method, the water extract showed strong antioxidant activity, but the methanol and acetone extracts did not exhibit significant activity. The water extract of rice bran was divided into the ethanol-precipitable (EP) and supernatant fractions, and EP fraction showed the dominant antioxidant activity, but the supernatant fraction did not exhibit significant antioxidant activity. When the effect of EP fraction on umu C gene expression in SOS response associated with DNA damage in Salmonella typhimurium (TA 1535/pSK 1002) induced by 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was analyzed, it showed a dose-dependent suppressive activity against Trp-P-1-induced umu C gene expression. The bio-chemical analysis of EP fraction indicates that the major antioxidative and antigenotoxic activity of EP fraction is associated with a proteinous component with the molecular weight of more than 30 KDa. As a possible active principle for the antioxidative and antigenotoxic activity in EP fraction, the strong activity of an oxygen radical-scavenging enzyme, peroxidase was detected, and the purified horseradish peroxidase also caused the similar antioxidative and antigenotoxic activities. The significance of this finding is discussed from the viewpoint of the preventive role of rice bran against oxygen radical-related chronic diseases. SN - 0951-418X UR - https://www.unboundmedicine.com/medline/citation/15476307/Potent_antioxidative_and_antigenotoxic_activity_in_aqueous_extract_of_Japanese_rice_bran__association_with_peroxidase_activity_ L2 - https://doi.org/10.1002/ptr.1576 DB - PRIME DP - Unbound Medicine ER -