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Microbial metabolites of ingested caffeic acid are absorbed by the monocarboxylic acid transporter (MCT) in intestinal Caco-2 cell monolayers.
J Agric Food Chem. 2004 Oct 20; 52(21):6418-24.JA

Abstract

It was previously reported that m-coumaric acid, m-hydroxyphenylpropionic acid (mHPP), and 3,4-dihydroxyphenylpropionic acid (DHPP) are major metabolites of ingested caffeic acid formed by gut microflora and would be transported by the monocarboxylic acid transporter (MCT). We have directly measured their absorption characteristics in Caco-2 cells using a coulometric detection method involving HPLC-ECD. The proton-coupled directional transport of m-coumaric acid, mHPP, and DHPP was observed, and the transport was inhibited by an MCT substrate. The permeation of m-coumaric acid and mHPP was concentration-dependent and saturable: The Michaelis constant for m-coumaric acid and mHPP was 32.5 and 12.9 mM, respectively, and the maximum velocity for m-coumaric acid and mHPP was 204.3 and 91.2 nmol (min)(-1) (mg protein)(-1), respectively. By contrast, the permeation of DHPP was nonsaturable even at 30 mM and was inversely correlated with the paracellular permeability of Caco-2 cells. Our results demonstrate that these compounds are absorbed by the MCT, although DHPP is mainly permeated across Caco-2 cells via the paracellular pathway. MCT-mediated absorption of phenolic compounds per se and their colonic metabolites would exert significant impact on human health.

Authors+Show Affiliations

Applied Bioresearch Center, Research & Development Department, Kirin Brewery Company, Ltd., 3 Miyaharacho, Takasaki-shi, Gunma 370-1295, Japan. konishiy@kirin.co.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15479001

Citation

Konishi, Yutaka, and Shoko Kobayashi. "Microbial Metabolites of Ingested Caffeic Acid Are Absorbed By the Monocarboxylic Acid Transporter (MCT) in Intestinal Caco-2 Cell Monolayers." Journal of Agricultural and Food Chemistry, vol. 52, no. 21, 2004, pp. 6418-24.
Konishi Y, Kobayashi S. Microbial metabolites of ingested caffeic acid are absorbed by the monocarboxylic acid transporter (MCT) in intestinal Caco-2 cell monolayers. J Agric Food Chem. 2004;52(21):6418-24.
Konishi, Y., & Kobayashi, S. (2004). Microbial metabolites of ingested caffeic acid are absorbed by the monocarboxylic acid transporter (MCT) in intestinal Caco-2 cell monolayers. Journal of Agricultural and Food Chemistry, 52(21), 6418-24.
Konishi Y, Kobayashi S. Microbial Metabolites of Ingested Caffeic Acid Are Absorbed By the Monocarboxylic Acid Transporter (MCT) in Intestinal Caco-2 Cell Monolayers. J Agric Food Chem. 2004 Oct 20;52(21):6418-24. PubMed PMID: 15479001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microbial metabolites of ingested caffeic acid are absorbed by the monocarboxylic acid transporter (MCT) in intestinal Caco-2 cell monolayers. AU - Konishi,Yutaka, AU - Kobayashi,Shoko, PY - 2004/10/14/pubmed PY - 2004/12/16/medline PY - 2004/10/14/entrez SP - 6418 EP - 24 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 21 N2 - It was previously reported that m-coumaric acid, m-hydroxyphenylpropionic acid (mHPP), and 3,4-dihydroxyphenylpropionic acid (DHPP) are major metabolites of ingested caffeic acid formed by gut microflora and would be transported by the monocarboxylic acid transporter (MCT). We have directly measured their absorption characteristics in Caco-2 cells using a coulometric detection method involving HPLC-ECD. The proton-coupled directional transport of m-coumaric acid, mHPP, and DHPP was observed, and the transport was inhibited by an MCT substrate. The permeation of m-coumaric acid and mHPP was concentration-dependent and saturable: The Michaelis constant for m-coumaric acid and mHPP was 32.5 and 12.9 mM, respectively, and the maximum velocity for m-coumaric acid and mHPP was 204.3 and 91.2 nmol (min)(-1) (mg protein)(-1), respectively. By contrast, the permeation of DHPP was nonsaturable even at 30 mM and was inversely correlated with the paracellular permeability of Caco-2 cells. Our results demonstrate that these compounds are absorbed by the MCT, although DHPP is mainly permeated across Caco-2 cells via the paracellular pathway. MCT-mediated absorption of phenolic compounds per se and their colonic metabolites would exert significant impact on human health. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15479001/Microbial_metabolites_of_ingested_caffeic_acid_are_absorbed_by_the_monocarboxylic_acid_transporter__MCT__in_intestinal_Caco_2_cell_monolayers_ L2 - https://doi.org/10.1021/jf049560y DB - PRIME DP - Unbound Medicine ER -