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Dynamics of COX-2 in nasal mucosa and nasal polyps from aspirin-tolerant and aspirin-intolerant patients with asthma.
J Allergy Clin Immunol. 2004 Oct; 114(4):814-9.JA

Abstract

BACKGROUND

Only dynamic studies can elucidate the discrepancies concerning the expression of the inducible COX-2 gene in inflammatory airway diseases.

OBJECTIVES

To quantify the expression and spontaneous regulation of COX-1 and COX-2 mRNAs in nasal polyps and nasal mucosa by real-time PCR.

METHODS

Nasal polyps were obtained from 16 aspirin-tolerant patients with asthma/rhinitis (ATAR) and 18 aspirin-intolerant patients with asthma/rhinitis (AIAR) undergoing nasal polypectomy. Nasal mucosa was obtained from 12 subjects undergoing nasal corrective surgery. All specimens were cut into 3 pieces. One was immediately snap-frozen in liquid nitrogen, and the remaining 2 were left at room temperature for 30 or 60 minutes before freezing. Data are presented as medians and 25th to 75th percentiles of 10 6 cDNA molecules/microg total RNA.

RESULTS

Baseline COX-2 mRNA levels were significantly lower in both ATAR (0.45; 0.13-1.20; P <.05) and AIAR (0.24; 0.12-0.41; P <.001) nasal polyps than in nasal mucosa (1.35; 0.52-3.90). COX-2 mRNA expression did not change over time in nasal mucosa but increased significantly in ATAR nasal polyps (P <.05), reaching similar levels to nasal mucosa after 60 minutes. In contrast, COX-2 mRNA showed no significant change over time in AIAR nasal polyps. COX-1 mRNA was higher in nasal polyps than in nasal mucosa, and its expression was not modified over time in any group of patients.

CONCLUSION

These results suggest differential kinetics of COX-2 mRNA between nasal mucosa and nasal polyps. AIAR nasal polyps appear to have a greater abnormality of the COX-2 pathway than ATAR.

Authors+Show Affiliations

Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15480320

Citation

Pujols, Laura, et al. "Dynamics of COX-2 in Nasal Mucosa and Nasal Polyps From Aspirin-tolerant and Aspirin-intolerant Patients With Asthma." The Journal of Allergy and Clinical Immunology, vol. 114, no. 4, 2004, pp. 814-9.
Pujols L, Mullol J, Alobid I, et al. Dynamics of COX-2 in nasal mucosa and nasal polyps from aspirin-tolerant and aspirin-intolerant patients with asthma. J Allergy Clin Immunol. 2004;114(4):814-9.
Pujols, L., Mullol, J., Alobid, I., Roca-Ferrer, J., Xaubet, A., & Picado, C. (2004). Dynamics of COX-2 in nasal mucosa and nasal polyps from aspirin-tolerant and aspirin-intolerant patients with asthma. The Journal of Allergy and Clinical Immunology, 114(4), 814-9.
Pujols L, et al. Dynamics of COX-2 in Nasal Mucosa and Nasal Polyps From Aspirin-tolerant and Aspirin-intolerant Patients With Asthma. J Allergy Clin Immunol. 2004;114(4):814-9. PubMed PMID: 15480320.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamics of COX-2 in nasal mucosa and nasal polyps from aspirin-tolerant and aspirin-intolerant patients with asthma. AU - Pujols,Laura, AU - Mullol,Joaquim, AU - Alobid,Isam, AU - Roca-Ferrer,Jordi, AU - Xaubet,Antoni, AU - Picado,César, PY - 2004/10/14/pubmed PY - 2004/12/31/medline PY - 2004/10/14/entrez SP - 814 EP - 9 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 114 IS - 4 N2 - BACKGROUND: Only dynamic studies can elucidate the discrepancies concerning the expression of the inducible COX-2 gene in inflammatory airway diseases. OBJECTIVES: To quantify the expression and spontaneous regulation of COX-1 and COX-2 mRNAs in nasal polyps and nasal mucosa by real-time PCR. METHODS: Nasal polyps were obtained from 16 aspirin-tolerant patients with asthma/rhinitis (ATAR) and 18 aspirin-intolerant patients with asthma/rhinitis (AIAR) undergoing nasal polypectomy. Nasal mucosa was obtained from 12 subjects undergoing nasal corrective surgery. All specimens were cut into 3 pieces. One was immediately snap-frozen in liquid nitrogen, and the remaining 2 were left at room temperature for 30 or 60 minutes before freezing. Data are presented as medians and 25th to 75th percentiles of 10 6 cDNA molecules/microg total RNA. RESULTS: Baseline COX-2 mRNA levels were significantly lower in both ATAR (0.45; 0.13-1.20; P <.05) and AIAR (0.24; 0.12-0.41; P <.001) nasal polyps than in nasal mucosa (1.35; 0.52-3.90). COX-2 mRNA expression did not change over time in nasal mucosa but increased significantly in ATAR nasal polyps (P <.05), reaching similar levels to nasal mucosa after 60 minutes. In contrast, COX-2 mRNA showed no significant change over time in AIAR nasal polyps. COX-1 mRNA was higher in nasal polyps than in nasal mucosa, and its expression was not modified over time in any group of patients. CONCLUSION: These results suggest differential kinetics of COX-2 mRNA between nasal mucosa and nasal polyps. AIAR nasal polyps appear to have a greater abnormality of the COX-2 pathway than ATAR. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15480320/Dynamics_of_COX_2_in_nasal_mucosa_and_nasal_polyps_from_aspirin_tolerant_and_aspirin_intolerant_patients_with_asthma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674904021694 DB - PRIME DP - Unbound Medicine ER -