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Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen.
J Allergy Clin Immunol 2004; 114(4):908-14JA

Abstract

BACKGROUND

Walnut is the most common cause of allergic reactions to tree nuts, as reported by large population studies. Two major allergens of walnut have been identified up until now: a 2S albumin and a vicilin-like protein.

OBJECTIVE

This study was designed to identify the walnut major allergens in the Italian population and to compare the walnut IgE-binding profile in patients with or without pollen allergy.

METHODS

We selected 46 patients either with oral allergy syndrome confirmed by open oral challenge or with systemic symptoms after ingestion of walnut. These patients' sera were used for the immunoblotting of walnut extract; the identified allergens were purified by HPLC and sequenced. A peach-walnut cross-inhibition study was then performed.

RESULTS

The only major allergen recognized by our study population was a 9-kd lipid transfer protein (LTP), recognized by 37 patients. Two other minor allergens of approximately 9-kd molecular weight, both belonging to the vicilin family, were recognized by 10 patients. IgE binding to walnut LTP was completely inhibited by peach LTP.

CONCLUSION

In Italian patients with walnut allergy confirmed by documented history of severe systemic reactions or by open oral food challenge, the major allergen is an LTP. The sensitization to this protein seems to be secondary to the sensitization to peach LTP, which acts as the primary sensitizer. LTP and vicilins were able to sensitize patients not allergic to pollen.

Authors+Show Affiliations

Allergy Center, Department of Internal Medicine and Dermatology, Ospedale Maggiore Istituto di Ricovero e Cura a Carattere Scientifico, Milan, Italy. elide.pastorello@unimi.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Multicenter Study

Language

eng

PubMed ID

15480333

Citation

Pastorello, Elide A., et al. "Lipid Transfer Protein and Vicilin Are Important Walnut Allergens in Patients Not Allergic to Pollen." The Journal of Allergy and Clinical Immunology, vol. 114, no. 4, 2004, pp. 908-14.
Pastorello EA, Farioli L, Pravettoni V, et al. Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. J Allergy Clin Immunol. 2004;114(4):908-14.
Pastorello, E. A., Farioli, L., Pravettoni, V., Robino, A. M., Scibilia, J., Fortunato, D., ... Ortolani, C. (2004). Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. The Journal of Allergy and Clinical Immunology, 114(4), pp. 908-14.
Pastorello EA, et al. Lipid Transfer Protein and Vicilin Are Important Walnut Allergens in Patients Not Allergic to Pollen. J Allergy Clin Immunol. 2004;114(4):908-14. PubMed PMID: 15480333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. AU - Pastorello,Elide A, AU - Farioli,Laura, AU - Pravettoni,Valerio, AU - Robino,Anna M, AU - Scibilia,Joseph, AU - Fortunato,Donatella, AU - Conti,Amedeo, AU - Borgonovo,Linda, AU - Bengtsson,Anders, AU - Ortolani,Claudio, PY - 2004/10/14/pubmed PY - 2004/12/31/medline PY - 2004/10/14/entrez SP - 908 EP - 14 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 114 IS - 4 N2 - BACKGROUND: Walnut is the most common cause of allergic reactions to tree nuts, as reported by large population studies. Two major allergens of walnut have been identified up until now: a 2S albumin and a vicilin-like protein. OBJECTIVE: This study was designed to identify the walnut major allergens in the Italian population and to compare the walnut IgE-binding profile in patients with or without pollen allergy. METHODS: We selected 46 patients either with oral allergy syndrome confirmed by open oral challenge or with systemic symptoms after ingestion of walnut. These patients' sera were used for the immunoblotting of walnut extract; the identified allergens were purified by HPLC and sequenced. A peach-walnut cross-inhibition study was then performed. RESULTS: The only major allergen recognized by our study population was a 9-kd lipid transfer protein (LTP), recognized by 37 patients. Two other minor allergens of approximately 9-kd molecular weight, both belonging to the vicilin family, were recognized by 10 patients. IgE binding to walnut LTP was completely inhibited by peach LTP. CONCLUSION: In Italian patients with walnut allergy confirmed by documented history of severe systemic reactions or by open oral food challenge, the major allergen is an LTP. The sensitization to this protein seems to be secondary to the sensitization to peach LTP, which acts as the primary sensitizer. LTP and vicilins were able to sensitize patients not allergic to pollen. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15480333/Lipid_transfer_protein_and_vicilin_are_important_walnut_allergens_in_patients_not_allergic_to_pollen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S009167490401721X DB - PRIME DP - Unbound Medicine ER -