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Fragmentation characteristics of permethylated oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight (TOF/TOF) tandem mass spectrometer.
Rapid Commun Mass Spectrom. 2004; 18(22):2637-49.RC

Abstract

Matrix-assisted laser desorption/ionization two-stage time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS/MS) was applied to characterize permethylated oligosaccharides. Under these ionization conditions such derivatives yield intense signals corresponding to sodium-cationized molecular species. A systematic study was conducted on a series of neutral and sialylated permethylated oligosaccharides to allow rationalization of the fragmentation processes. The major fragments observed in the MALDI-TOF/TOF-MS/MS spectra result from cleavage of glycosidic bonds, preferentially at N-acetylhexosamine and sialic acid residues. The fragments originating from both the reducing and the non-reducing ends of the glycan yield information on sequence and branching. Cross-ring cleavages, which are very informative of the linkages of the monosaccharide residues constituting these oligosaccharides, and 'internal' cleavage ions which are derived from elimination of substituents from around the pyranose ring, were also observed. This extensive fragmentation was shown to be useful for the structural characterization of oligosaccharides. MALDI-TOF/TOF-MS/MS of permethylated oligosaccharides appears to be a powerful tool for carbohydrate structural analysis.

Authors+Show Affiliations

Unité Mixte de Recherche CNRS/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq, France. willy.morelle@univ-lille1.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15481102

Citation

Morelle, Willy, et al. "Fragmentation Characteristics of Permethylated Oligosaccharides Using a Matrix-assisted Laser Desorption/ionization Two-stage Time-of-flight (TOF/TOF) Tandem Mass Spectrometer." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 22, 2004, pp. 2637-49.
Morelle W, Slomianny MC, Diemer H, et al. Fragmentation characteristics of permethylated oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight (TOF/TOF) tandem mass spectrometer. Rapid Commun Mass Spectrom. 2004;18(22):2637-49.
Morelle, W., Slomianny, M. C., Diemer, H., Schaeffer, C., van Dorsselaer, A., & Michalski, J. C. (2004). Fragmentation characteristics of permethylated oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight (TOF/TOF) tandem mass spectrometer. Rapid Communications in Mass Spectrometry : RCM, 18(22), 2637-49.
Morelle W, et al. Fragmentation Characteristics of Permethylated Oligosaccharides Using a Matrix-assisted Laser Desorption/ionization Two-stage Time-of-flight (TOF/TOF) Tandem Mass Spectrometer. Rapid Commun Mass Spectrom. 2004;18(22):2637-49. PubMed PMID: 15481102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fragmentation characteristics of permethylated oligosaccharides using a matrix-assisted laser desorption/ionization two-stage time-of-flight (TOF/TOF) tandem mass spectrometer. AU - Morelle,Willy, AU - Slomianny,Marie-Christine, AU - Diemer,Hélène, AU - Schaeffer,Christine, AU - van Dorsselaer,Alain, AU - Michalski,Jean-Claude, PY - 2004/10/14/pubmed PY - 2004/12/16/medline PY - 2004/10/14/entrez SP - 2637 EP - 49 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 22 N2 - Matrix-assisted laser desorption/ionization two-stage time-of-flight (MALDI-TOF/TOF) tandem mass spectrometry (MS/MS) was applied to characterize permethylated oligosaccharides. Under these ionization conditions such derivatives yield intense signals corresponding to sodium-cationized molecular species. A systematic study was conducted on a series of neutral and sialylated permethylated oligosaccharides to allow rationalization of the fragmentation processes. The major fragments observed in the MALDI-TOF/TOF-MS/MS spectra result from cleavage of glycosidic bonds, preferentially at N-acetylhexosamine and sialic acid residues. The fragments originating from both the reducing and the non-reducing ends of the glycan yield information on sequence and branching. Cross-ring cleavages, which are very informative of the linkages of the monosaccharide residues constituting these oligosaccharides, and 'internal' cleavage ions which are derived from elimination of substituents from around the pyranose ring, were also observed. This extensive fragmentation was shown to be useful for the structural characterization of oligosaccharides. MALDI-TOF/TOF-MS/MS of permethylated oligosaccharides appears to be a powerful tool for carbohydrate structural analysis. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15481102/Fragmentation_characteristics_of_permethylated_oligosaccharides_using_a_matrix_assisted_laser_desorption/ionization_two_stage_time_of_flight__TOF/TOF__tandem_mass_spectrometer_ L2 - https://doi.org/10.1002/rcm.1668 DB - PRIME DP - Unbound Medicine ER -