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Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets.
Fundam Clin Pharmacol. 2004 Oct; 18(5):525-9.FC

Abstract

Nigella sativa L. 'Black cumin' (Ranunculaceae) is one of the plants commonly used in Moroccan folk medicine for treatment of various ailments including diabetes mellitus. The present study was undertaken to investigate the effect of different N. sativa seed extracts on insulin secretion. Different fractions of the seed were prepared: the defatted fraction (HR II), which was divided into two subfractions: the first (HR III) containing acidic and neutral compounds and the second (HR IV) containing basic compounds. The insulin secretory effects of these extracts were evaluated individually at different concentrations (0.01, 0.1, 1 and 5 mg/mL), in vitro in isolated rat pancreatic islets in the presence of 8.3 mmol/L glucose. The results show that addition of the defatted whole extract or of the basic subfraction of the seed in the incubation medium significantly increased glucose-induced insulin release from the islets. In the case of the acidic and neutral subfraction, the stimulatory effect was observed only for the higher concentration (5 mg/mL). However, a clear concentration-dependent increase in insulin release from isolated pancreatic islets was observed for the basic subfraction. Our data show that the antidiabetic properties of N. sativa seeds may be, at least partly, mediated by stimulated insulin release, and that the basic subfraction largely contributes to this stimulatory effect. Further phytochemical studies are underway in order to isolate the pharmacological compound(s) responsible for the insulinotropic effect of N. sativa seeds.

Authors+Show Affiliations

Laboratoire de Recherche sur les Substances Naturelles Végétales, Université Montpellier II UPRES EA 1677, Place E. Bataillon, Bât. 15, CC 024, 34095 Montpellier Cedex 05, France. rchidhalima@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15482373

Citation

Rchid, Halima, et al. "Nigella Sativa Seed Extracts Enhance Glucose-induced Insulin Release From Rat-isolated Langerhans Islets." Fundamental & Clinical Pharmacology, vol. 18, no. 5, 2004, pp. 525-9.
Rchid H, Chevassus H, Nmila R, et al. Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets. Fundam Clin Pharmacol. 2004;18(5):525-9.
Rchid, H., Chevassus, H., Nmila, R., Guiral, C., Petit, P., Chokaïri, M., & Sauvaire, Y. (2004). Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets. Fundamental & Clinical Pharmacology, 18(5), 525-9.
Rchid H, et al. Nigella Sativa Seed Extracts Enhance Glucose-induced Insulin Release From Rat-isolated Langerhans Islets. Fundam Clin Pharmacol. 2004;18(5):525-9. PubMed PMID: 15482373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nigella sativa seed extracts enhance glucose-induced insulin release from rat-isolated Langerhans islets. AU - Rchid,Halima, AU - Chevassus,Hugues, AU - Nmila,Rachid, AU - Guiral,Carine, AU - Petit,Pierre, AU - Chokaïri,Mustapha, AU - Sauvaire,Yves, PY - 2004/10/16/pubmed PY - 2005/2/18/medline PY - 2004/10/16/entrez SP - 525 EP - 9 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 18 IS - 5 N2 - Nigella sativa L. 'Black cumin' (Ranunculaceae) is one of the plants commonly used in Moroccan folk medicine for treatment of various ailments including diabetes mellitus. The present study was undertaken to investigate the effect of different N. sativa seed extracts on insulin secretion. Different fractions of the seed were prepared: the defatted fraction (HR II), which was divided into two subfractions: the first (HR III) containing acidic and neutral compounds and the second (HR IV) containing basic compounds. The insulin secretory effects of these extracts were evaluated individually at different concentrations (0.01, 0.1, 1 and 5 mg/mL), in vitro in isolated rat pancreatic islets in the presence of 8.3 mmol/L glucose. The results show that addition of the defatted whole extract or of the basic subfraction of the seed in the incubation medium significantly increased glucose-induced insulin release from the islets. In the case of the acidic and neutral subfraction, the stimulatory effect was observed only for the higher concentration (5 mg/mL). However, a clear concentration-dependent increase in insulin release from isolated pancreatic islets was observed for the basic subfraction. Our data show that the antidiabetic properties of N. sativa seeds may be, at least partly, mediated by stimulated insulin release, and that the basic subfraction largely contributes to this stimulatory effect. Further phytochemical studies are underway in order to isolate the pharmacological compound(s) responsible for the insulinotropic effect of N. sativa seeds. SN - 0767-3981 UR - https://www.unboundmedicine.com/medline/citation/15482373/Nigella_sativa_seed_extracts_enhance_glucose_induced_insulin_release_from_rat_isolated_Langerhans_islets_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0767-3981&date=2004&volume=18&issue=5&spage=525 DB - PRIME DP - Unbound Medicine ER -