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Characterization of a bacteriocin produced by Prevotella nigrescens ATCC 25261.
Lett Appl Microbiol. 2004; 39(5):451-8.LA

Abstract

AIMS

To characterize the antimicrobial activity produced by Prevotella nigrescens ATCC 25261, and to evaluate its safety on cultured gingival fibroblasts.

METHODS AND RESULTS

An antimicrobial activity was obtained from purifying the culture supernatant of Pr. nigrescens ATCC 25261. Purification of the active compound was achieved with ammonium sulphate precipitation followed by anion-exchange and gel filtration chromatography. As revealed by SDS-PAGE, the active fraction was relatively homogeneous, showing a protein with an approximate molecular weight of 41 kDa. The antimicrobial compound, named nigrescin, exhibited a bactericidal mode of action against Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Actinomyces spp. Nigrescin was stable in a pH range between 6.5 and 9.5, at 100 degrees C for 10 min, and resistant to lyophilization. But its activity was lost after proteinase K treatment. Despite at very high concentrations beyond the minimum inhibitory concentration (MIC), nigrescin was not toxic to the gingival fibroblasts.

CONCLUSION

Nigrescin is a novel bacteriocin produced by Pr. nigrescens ATCC 25261. It exhibits antimicrobial activity against species that are implicated in periodontal diseases. The absence of toxicity on the gingival fibroblasts suggests the possibility in using of nigrescin for an application in periodontal treatment.

SIGNIFICANCE AND IMPACT OF THE STUDY

Novel evidence on nigrescin would make Pr. nigrescens ATCC 25261 attractive in biotechnological applications as an antimicrobial agent in clinical dentistry.

Authors+Show Affiliations

Department of Stomatology, Faculty of Dentistry, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. mjasdee@ratree.psu.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15482437

Citation

Kaewsrichan, J, et al. "Characterization of a Bacteriocin Produced By Prevotella Nigrescens ATCC 25261." Letters in Applied Microbiology, vol. 39, no. 5, 2004, pp. 451-8.
Kaewsrichan J, Douglas CW, Nissen-Meyer J, et al. Characterization of a bacteriocin produced by Prevotella nigrescens ATCC 25261. Lett Appl Microbiol. 2004;39(5):451-8.
Kaewsrichan, J., Douglas, C. W., Nissen-Meyer, J., Fimland, G., & Teanpaisan, R. (2004). Characterization of a bacteriocin produced by Prevotella nigrescens ATCC 25261. Letters in Applied Microbiology, 39(5), 451-8.
Kaewsrichan J, et al. Characterization of a Bacteriocin Produced By Prevotella Nigrescens ATCC 25261. Lett Appl Microbiol. 2004;39(5):451-8. PubMed PMID: 15482437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a bacteriocin produced by Prevotella nigrescens ATCC 25261. AU - Kaewsrichan,J, AU - Douglas,C W I, AU - Nissen-Meyer,J, AU - Fimland,G, AU - Teanpaisan,R, PY - 2004/10/16/pubmed PY - 2005/2/18/medline PY - 2004/10/16/entrez SP - 451 EP - 8 JF - Letters in applied microbiology JO - Lett. Appl. Microbiol. VL - 39 IS - 5 N2 - AIMS: To characterize the antimicrobial activity produced by Prevotella nigrescens ATCC 25261, and to evaluate its safety on cultured gingival fibroblasts. METHODS AND RESULTS: An antimicrobial activity was obtained from purifying the culture supernatant of Pr. nigrescens ATCC 25261. Purification of the active compound was achieved with ammonium sulphate precipitation followed by anion-exchange and gel filtration chromatography. As revealed by SDS-PAGE, the active fraction was relatively homogeneous, showing a protein with an approximate molecular weight of 41 kDa. The antimicrobial compound, named nigrescin, exhibited a bactericidal mode of action against Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Actinomyces spp. Nigrescin was stable in a pH range between 6.5 and 9.5, at 100 degrees C for 10 min, and resistant to lyophilization. But its activity was lost after proteinase K treatment. Despite at very high concentrations beyond the minimum inhibitory concentration (MIC), nigrescin was not toxic to the gingival fibroblasts. CONCLUSION: Nigrescin is a novel bacteriocin produced by Pr. nigrescens ATCC 25261. It exhibits antimicrobial activity against species that are implicated in periodontal diseases. The absence of toxicity on the gingival fibroblasts suggests the possibility in using of nigrescin for an application in periodontal treatment. SIGNIFICANCE AND IMPACT OF THE STUDY: Novel evidence on nigrescin would make Pr. nigrescens ATCC 25261 attractive in biotechnological applications as an antimicrobial agent in clinical dentistry. SN - 0266-8254 UR - https://www.unboundmedicine.com/medline/citation/15482437/Characterization_of_a_bacteriocin_produced_by_Prevotella_nigrescens_ATCC_25261_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0266-8254&date=2004&volume=39&issue=5&spage=451 DB - PRIME DP - Unbound Medicine ER -