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Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma.
J Gastroenterol Hepatol. 2004 Nov; 19(11):1300-4.JG

Abstract

BACKGROUND AND AIM

Telomerase activation is essential for the immortality of cancer cells. The expression of telomerase reverse transcriptase (hTERT), the catalytic component of the telomerase complex, regulates telomerase activity in human cancers. Amplification of the hTERT gene, located at chromosome 5p, is thought to be a potential genetic event contributing to telomerase activation in sporadic tumors.

METHODS

The amplification of the hTERT gene was examined in 46 surgically resected hepatocellular carcinomas (HCC) by real-time polymerase chain reaction and the status was compared with the expression of hTERT mRNA and clinicopathological parameters.

RESULTS

Amplified hTERT genes were found in 21.7% (10/46) of HCC. The incidence of amplified hTERT genes in poorly differentiated HCC (6/12, 50%) was significantly higher than that in highly to moderately differentiated HCC (4/34, 11.8%; P = 0.012). Tumor size in those cases with hTERT gene amplification was larger compared to those cases with no amplification (P = 0.047). Amplification of the hTERT gene was not observed in non-cancerous tissues. The hTERT mRNA level did not correlate with the number of hTERT genes.

CONCLUSIONS

Based on these results, it is thought that hTERT gene amplification is a cancer-specific event, and may furthermore contribute to the dedifferentiation and development of HCC. However, hTERT gene overexpression was rarely due to an increased hTERT gene copy number in HCC.

Authors+Show Affiliations

Department of Medicine and Medical Science, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan. takuma@enjoy.ne.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15482538

Citation

Takuma, Yoshitaka, et al. "Telomerase Reverse Transcriptase Gene Amplification in Hepatocellular Carcinoma." Journal of Gastroenterology and Hepatology, vol. 19, no. 11, 2004, pp. 1300-4.
Takuma Y, Nouso K, Kobayashi Y, et al. Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma. J Gastroenterol Hepatol. 2004;19(11):1300-4.
Takuma, Y., Nouso, K., Kobayashi, Y., Nakamura, S., Tanaka, H., Matsumoto, E., Fujikawa, T., Suzuki, M., Hanafusa, T., & Shiratori, Y. (2004). Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma. Journal of Gastroenterology and Hepatology, 19(11), 1300-4.
Takuma Y, et al. Telomerase Reverse Transcriptase Gene Amplification in Hepatocellular Carcinoma. J Gastroenterol Hepatol. 2004;19(11):1300-4. PubMed PMID: 15482538.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma. AU - Takuma,Yoshitaka, AU - Nouso,Kazuhiro, AU - Kobayashi,Yoshiyuki, AU - Nakamura,Shinichiro, AU - Tanaka,Hironori, AU - Matsumoto,Eiji, AU - Fujikawa,Tatsuya, AU - Suzuki,Mayumi, AU - Hanafusa,Tadashi, AU - Shiratori,Yasushi, PY - 2004/10/16/pubmed PY - 2005/3/23/medline PY - 2004/10/16/entrez SP - 1300 EP - 4 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 19 IS - 11 N2 - BACKGROUND AND AIM: Telomerase activation is essential for the immortality of cancer cells. The expression of telomerase reverse transcriptase (hTERT), the catalytic component of the telomerase complex, regulates telomerase activity in human cancers. Amplification of the hTERT gene, located at chromosome 5p, is thought to be a potential genetic event contributing to telomerase activation in sporadic tumors. METHODS: The amplification of the hTERT gene was examined in 46 surgically resected hepatocellular carcinomas (HCC) by real-time polymerase chain reaction and the status was compared with the expression of hTERT mRNA and clinicopathological parameters. RESULTS: Amplified hTERT genes were found in 21.7% (10/46) of HCC. The incidence of amplified hTERT genes in poorly differentiated HCC (6/12, 50%) was significantly higher than that in highly to moderately differentiated HCC (4/34, 11.8%; P = 0.012). Tumor size in those cases with hTERT gene amplification was larger compared to those cases with no amplification (P = 0.047). Amplification of the hTERT gene was not observed in non-cancerous tissues. The hTERT mRNA level did not correlate with the number of hTERT genes. CONCLUSIONS: Based on these results, it is thought that hTERT gene amplification is a cancer-specific event, and may furthermore contribute to the dedifferentiation and development of HCC. However, hTERT gene overexpression was rarely due to an increased hTERT gene copy number in HCC. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/15482538/Telomerase_reverse_transcriptase_gene_amplification_in_hepatocellular_carcinoma_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0815-9319&date=2004&volume=19&issue=11&spage=1300 DB - PRIME DP - Unbound Medicine ER -