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The 1.2-megabase genome sequence of Mimivirus.
Science. 2004 Nov 19; 306(5700):1344-50.Sci

Abstract

We recently reported the discovery and preliminary characterization of Mimivirus, the largest known virus, with a 400-nanometer particle size comparable to mycoplasma. Mimivirus is a double-stranded DNA virus growing in amoebae. We now present its 1,181,404-base pair genome sequence, consisting of 1262 putative open reading frames, 10% of which exhibit a similarity to proteins of known functions. In addition to exceptional genome size, Mimivirus exhibits many features that distinguish it from other nucleocytoplasmic large DNA viruses. The most unexpected is the presence of numerous genes encoding central protein-translation components, including four amino-acyl transfer RNA synthetases, peptide release factor 1, translation elongation factor EF-TU, and translation initiation factor 1. The genome also exhibits six tRNAs. Other notable features include the presence of both type I and type II topoisomerases, components of all DNA repair pathways, many polysaccharide synthesis enzymes, and one intein-containing gene. The size and complexity of the Mimivirus genome challenge the established frontier between viruses and parasitic cellular organisms. This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes.

Authors+Show Affiliations

Unité des Rickettsies, Faculté de Médecine, CNRS UMR6020, Université de la Méditerranée, 13385 Marseille Cedex 05, France. Didier.Raoult@medecine.univ-mrs.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15486256

Citation

Raoult, Didier, et al. "The 1.2-megabase Genome Sequence of Mimivirus." Science (New York, N.Y.), vol. 306, no. 5700, 2004, pp. 1344-50.
Raoult D, Audic S, Robert C, et al. The 1.2-megabase genome sequence of Mimivirus. Science. 2004;306(5700):1344-50.
Raoult, D., Audic, S., Robert, C., Abergel, C., Renesto, P., Ogata, H., La Scola, B., Suzan, M., & Claverie, J. M. (2004). The 1.2-megabase genome sequence of Mimivirus. Science (New York, N.Y.), 306(5700), 1344-50.
Raoult D, et al. The 1.2-megabase Genome Sequence of Mimivirus. Science. 2004 Nov 19;306(5700):1344-50. PubMed PMID: 15486256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The 1.2-megabase genome sequence of Mimivirus. AU - Raoult,Didier, AU - Audic,Stéphane, AU - Robert,Catherine, AU - Abergel,Chantal, AU - Renesto,Patricia, AU - Ogata,Hiroyuki, AU - La Scola,Bernard, AU - Suzan,Marie, AU - Claverie,Jean-Michel, Y1 - 2004/10/14/ PY - 2004/10/16/pubmed PY - 2004/12/17/medline PY - 2004/10/16/entrez SP - 1344 EP - 50 JF - Science (New York, N.Y.) JO - Science VL - 306 IS - 5700 N2 - We recently reported the discovery and preliminary characterization of Mimivirus, the largest known virus, with a 400-nanometer particle size comparable to mycoplasma. Mimivirus is a double-stranded DNA virus growing in amoebae. We now present its 1,181,404-base pair genome sequence, consisting of 1262 putative open reading frames, 10% of which exhibit a similarity to proteins of known functions. In addition to exceptional genome size, Mimivirus exhibits many features that distinguish it from other nucleocytoplasmic large DNA viruses. The most unexpected is the presence of numerous genes encoding central protein-translation components, including four amino-acyl transfer RNA synthetases, peptide release factor 1, translation elongation factor EF-TU, and translation initiation factor 1. The genome also exhibits six tRNAs. Other notable features include the presence of both type I and type II topoisomerases, components of all DNA repair pathways, many polysaccharide synthesis enzymes, and one intein-containing gene. The size and complexity of the Mimivirus genome challenge the established frontier between viruses and parasitic cellular organisms. This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/15486256/The_1_2_megabase_genome_sequence_of_Mimivirus_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=15486256 DB - PRIME DP - Unbound Medicine ER -