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Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay.
J Virol Methods. 2004 Dec 01; 122(1):29-36.JV

Abstract

The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.

Links

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Authors+Show Affiliations

Adachi D
Division of Microbiology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ont., Canada M5G 1X8.
Johnson G
No affiliation info available
Draker R
No affiliation info available
Ayers M
No affiliation info available
Mazzulli T
No affiliation info available
Talbot PJ
No affiliation info available
Tellier R
No affiliation info available

MeSH

Base SequenceCoronavirusCoronavirus 229E, HumanCoronavirus InfectionsCoronavirus OC43, HumanDNA, ComplementaryDNA, ViralDeoxyribonucleases, Type II Site-SpecificHumansMolecular Sequence DataPhylogenyReverse Transcriptase Polymerase Chain ReactionSevere acute respiratory syndrome-related coronavirusSensitivity and SpecificitySequence AlignmentSequence Analysis, DNASevere Acute Respiratory Syndrome

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15488617

Citation

Adachi, D, et al. "Comprehensive Detection and Identification of Human Coronaviruses, Including the SARS-associated Coronavirus, With a Single RT-PCR Assay." Journal of Virological Methods, vol. 122, no. 1, 2004, pp. 29-36.
Adachi D, Johnson G, Draker R, et al. Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay. J Virol Methods. 2004;122(1):29-36.
Adachi, D., Johnson, G., Draker, R., Ayers, M., Mazzulli, T., Talbot, P. J., & Tellier, R. (2004). Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay. Journal of Virological Methods, 122(1), 29-36.
Adachi D, et al. Comprehensive Detection and Identification of Human Coronaviruses, Including the SARS-associated Coronavirus, With a Single RT-PCR Assay. J Virol Methods. 2004 Dec 1;122(1):29-36. PubMed PMID: 15488617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay. AU - Adachi,D, AU - Johnson,G, AU - Draker,R, AU - Ayers,M, AU - Mazzulli,T, AU - Talbot,P J, AU - Tellier,R, PY - 2004/06/11/received PY - 2004/07/17/revised PY - 2004/07/28/accepted PY - 2004/10/19/pubmed PY - 2005/3/23/medline PY - 2004/10/19/entrez SP - 29 EP - 36 JF - Journal of virological methods JO - J Virol Methods VL - 122 IS - 1 N2 - The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/15488617/Comprehensive_detection_and_identification_of_human_coronaviruses_including_the_SARS_associated_coronavirus_with_a_single_RT_PCR_assay_ DB - PRIME DP - Unbound Medicine ER -