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Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction.
Life Sci. 2004 Nov 12; 75(26):3157-71.LS

Abstract

Homocysteine (Hcy) is a by-product of methionine metabolism. An imbalance of Hcy in the body may lead to hyperhomocysteinemia, a condition with elevated Hcy concentration in blood that may be one of the risk factors responsible for the development of several vascular diseases (thromboembolism, atherosclerosis, stroke, vascular diseases and dementia). Radix Salvia miltiorrhiza (Danshen), a well-known Chinese medicinal herb that can activate and improve blood microcirculation, is noticeable for its beneficial effect in treating cardiovascular diseases. The present study is to demonstrate the protective effect of Danshen extract against the homocysteine-induced adverse effect on human umbilical vein endothelial cell (HUVEC). Homocysteine (5 mM) not only decreased the cell viability but also caused the disruption of capillary-like structure formation in vitro. The protective effect of Danshen aqueous extract and its active compounds on endothelial cell function were demonstrated through an in vitro tube formation assay, which mimics the new blood vessel formation. To identify the active components in the aqueous extract of Danshen, the content was characterized by instrumental analysis using high performance liquid chromatography with diode array detector (DAD) and electrospray tandem mass spectrometry (ESI-MS/MS). Interestingly, Danshen extract and its pure compounds showed different effectiveness in protecting HUVEC against Hcy-induced injury according to the following descending order: Danshen aqueous extract, 3-(3,4-dihydroxy-phenyl)-2-hydroxy-propionic acid (Danshensu), protocatechuic acid, catechin and protocatechualdehyde. We believed that such findings might provide evidence in understanding the beneficial effects of Danshen on the cardiovascular system.

Authors+Show Affiliations

Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15488895

Citation

Chan, K, et al. "Protective Effects of Danshensu From the Aqueous Extract of Salvia Miltiorrhiza (Danshen) Against Homocysteine-induced Endothelial Dysfunction." Life Sciences, vol. 75, no. 26, 2004, pp. 3157-71.
Chan K, Chui SH, Wong DY, et al. Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction. Life Sci. 2004;75(26):3157-71.
Chan, K., Chui, S. H., Wong, D. Y., Ha, W. Y., Chan, C. L., & Wong, R. N. (2004). Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction. Life Sciences, 75(26), 3157-71.
Chan K, et al. Protective Effects of Danshensu From the Aqueous Extract of Salvia Miltiorrhiza (Danshen) Against Homocysteine-induced Endothelial Dysfunction. Life Sci. 2004 Nov 12;75(26):3157-71. PubMed PMID: 15488895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of Danshensu from the aqueous extract of Salvia miltiorrhiza (Danshen) against homocysteine-induced endothelial dysfunction. AU - Chan,K, AU - Chui,S H, AU - Wong,D Y L, AU - Ha,W Y, AU - Chan,C L, AU - Wong,R N S, PY - 2003/11/17/received PY - 2004/06/14/accepted PY - 2004/10/19/pubmed PY - 2005/1/4/medline PY - 2004/10/19/entrez SP - 3157 EP - 71 JF - Life sciences JO - Life Sci. VL - 75 IS - 26 N2 - Homocysteine (Hcy) is a by-product of methionine metabolism. An imbalance of Hcy in the body may lead to hyperhomocysteinemia, a condition with elevated Hcy concentration in blood that may be one of the risk factors responsible for the development of several vascular diseases (thromboembolism, atherosclerosis, stroke, vascular diseases and dementia). Radix Salvia miltiorrhiza (Danshen), a well-known Chinese medicinal herb that can activate and improve blood microcirculation, is noticeable for its beneficial effect in treating cardiovascular diseases. The present study is to demonstrate the protective effect of Danshen extract against the homocysteine-induced adverse effect on human umbilical vein endothelial cell (HUVEC). Homocysteine (5 mM) not only decreased the cell viability but also caused the disruption of capillary-like structure formation in vitro. The protective effect of Danshen aqueous extract and its active compounds on endothelial cell function were demonstrated through an in vitro tube formation assay, which mimics the new blood vessel formation. To identify the active components in the aqueous extract of Danshen, the content was characterized by instrumental analysis using high performance liquid chromatography with diode array detector (DAD) and electrospray tandem mass spectrometry (ESI-MS/MS). Interestingly, Danshen extract and its pure compounds showed different effectiveness in protecting HUVEC against Hcy-induced injury according to the following descending order: Danshen aqueous extract, 3-(3,4-dihydroxy-phenyl)-2-hydroxy-propionic acid (Danshensu), protocatechuic acid, catechin and protocatechualdehyde. We believed that such findings might provide evidence in understanding the beneficial effects of Danshen on the cardiovascular system. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15488895/Protective_effects_of_Danshensu_from_the_aqueous_extract_of_Salvia_miltiorrhiza__Danshen__against_homocysteine_induced_endothelial_dysfunction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(04)00758-1 DB - PRIME DP - Unbound Medicine ER -