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Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons.
Neuroscience. 2004; 129(1):35-48.N

Abstract

Dorsal root ganglion neurons express functional AMPA and kainate receptors near their central terminals. Activation of these receptors causes a decrease in glutamate release during action potential evoked synaptic transmission. Due to differences in kinetic properties and expression patterns of these two families of glutamate receptors in subpopulations of sensory neurons, AMPA and kainate receptors are expected to function differently. We used embryonic dorsal root ganglion (DRG) neurons maintained in culture to compare functional properties of kainate and AMPA receptors. Most DRG neurons in culture expressed kainate receptors and about half also expressed AMPA receptors. Most AMPA and kainate receptor-expressing DRG neurons were sensitive to capsaicin, suggesting involvement of these glutamate receptors in nociception. When activated by kainate, AMPA receptors were capable of driving a sustained train of action potentials while kainate receptors tended to activate action potential firing more transiently. Glutamate elicited more action potentials and a larger steady-state depolarization in neurons expressing both AMPA and kainate receptors than in neurons expressing only kainate receptors. Adding to their more potent activation properties, AMPA receptors recovered from desensitization much more quickly than kainate receptors. Activation of presynaptic receptors by low concentrations of kainate, but not ATPA, caused a tetrodotoxin-sensitive increase in the frequency of spontaneous EPSCs recorded in dorsal horn neurons. By recording synaptic pairs of DRG and dorsal horn neurons, we found that activation of presynaptic kainate and AMPA receptors decreased evoked glutamate release from terminals of DRG neurons in culture. Our data suggest that the endogenous ligand, glutamate, will cause a different physiological impact when activating these two types of non-NMDA glutamate receptors at central or peripheral nerve endings of sensory neurons.

Authors+Show Affiliations

Department of Physiology and Cellular Biophysics and the Center for Neurobiology and Behavior, Columbia University, 630 West 168(th) Street BB1106, New York, NY 10032, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15489026

Citation

Lee, C J., et al. "Functional Similarities and Differences of AMPA and Kainate Receptors Expressed By Cultured Rat Sensory Neurons." Neuroscience, vol. 129, no. 1, 2004, pp. 35-48.
Lee CJ, Labrakakis C, Joseph DJ, et al. Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons. Neuroscience. 2004;129(1):35-48.
Lee, C. J., Labrakakis, C., Joseph, D. J., & Macdermott, A. B. (2004). Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons. Neuroscience, 129(1), 35-48.
Lee CJ, et al. Functional Similarities and Differences of AMPA and Kainate Receptors Expressed By Cultured Rat Sensory Neurons. Neuroscience. 2004;129(1):35-48. PubMed PMID: 15489026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional similarities and differences of AMPA and kainate receptors expressed by cultured rat sensory neurons. AU - Lee,C J, AU - Labrakakis,C, AU - Joseph,D J, AU - Macdermott,A B, PY - 2004/07/08/accepted PY - 2004/10/19/pubmed PY - 2005/1/14/medline PY - 2004/10/19/entrez SP - 35 EP - 48 JF - Neuroscience JO - Neuroscience VL - 129 IS - 1 N2 - Dorsal root ganglion neurons express functional AMPA and kainate receptors near their central terminals. Activation of these receptors causes a decrease in glutamate release during action potential evoked synaptic transmission. Due to differences in kinetic properties and expression patterns of these two families of glutamate receptors in subpopulations of sensory neurons, AMPA and kainate receptors are expected to function differently. We used embryonic dorsal root ganglion (DRG) neurons maintained in culture to compare functional properties of kainate and AMPA receptors. Most DRG neurons in culture expressed kainate receptors and about half also expressed AMPA receptors. Most AMPA and kainate receptor-expressing DRG neurons were sensitive to capsaicin, suggesting involvement of these glutamate receptors in nociception. When activated by kainate, AMPA receptors were capable of driving a sustained train of action potentials while kainate receptors tended to activate action potential firing more transiently. Glutamate elicited more action potentials and a larger steady-state depolarization in neurons expressing both AMPA and kainate receptors than in neurons expressing only kainate receptors. Adding to their more potent activation properties, AMPA receptors recovered from desensitization much more quickly than kainate receptors. Activation of presynaptic receptors by low concentrations of kainate, but not ATPA, caused a tetrodotoxin-sensitive increase in the frequency of spontaneous EPSCs recorded in dorsal horn neurons. By recording synaptic pairs of DRG and dorsal horn neurons, we found that activation of presynaptic kainate and AMPA receptors decreased evoked glutamate release from terminals of DRG neurons in culture. Our data suggest that the endogenous ligand, glutamate, will cause a different physiological impact when activating these two types of non-NMDA glutamate receptors at central or peripheral nerve endings of sensory neurons. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/15489026/Functional_similarities_and_differences_of_AMPA_and_kainate_receptors_expressed_by_cultured_rat_sensory_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(04)00620-7 DB - PRIME DP - Unbound Medicine ER -