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Effect of growth factors on proliferation and phenotypic differentiation of human fetal neural stem cells.
J Neurosci Res. 2004 Dec 01; 78(5):625-36.JN

Abstract

Human fetal neural stem cells (hNSCs) can be expanded in vitro by mitogens or growth factors, such as basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and/or leukemia inhibitory factor (LIF). Their effects on proliferation rate and differentiation pattern of hNSCs, however, have not been fully characterized. In this study, we cultured hNSCs in seven regimens, including bFGF, EGF, and LIF, either alone or in combinations. Cells were maintained as neurospheres in treatment media for various periods, up to six passages. A combination of bFGF, EGF, and LIF expanded hNSCs more efficiently than any other treatment as determined by counting total cell numbers using a trypan blue exclusion assay, a WST-1 cell viability assay, and a bromodeoxyuridine incorporation flow cytometric analysis. Differentiation patterns of hNSCs expanded under different conditions were also analyzed. We reported previously that hNSCs primed in vitro with a combination of bFGF, heparin, and laminin (FHL) induced neuronal differentiation toward a cholinergic phenotype. In this study, we show that the FHL priming increases neuronal differentiation while decreasing astroglial generation in all treatment groups as determined by immunostaining. However, cells proliferated under different growth factor conditions do vary in their phenotypic differentiation patterns. Particularly, significant generation of cholinergic cells was observed only in hNSCs expanded with EGF/bFGF or EGF/bFGF/LIF, but not with other treatment regimens, even when they are exposed to the same priming procedure. Our results indicate that hNSCs are highly plastic, with their proliferation and differentiation potential dependent on different growth factor treatments.

Authors+Show Affiliations

Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, Texas 77555-1043, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15490463

Citation

Tarasenko, Yevgeniya I., et al. "Effect of Growth Factors On Proliferation and Phenotypic Differentiation of Human Fetal Neural Stem Cells." Journal of Neuroscience Research, vol. 78, no. 5, 2004, pp. 625-36.
Tarasenko YI, Yu Y, Jordan PM, et al. Effect of growth factors on proliferation and phenotypic differentiation of human fetal neural stem cells. J Neurosci Res. 2004;78(5):625-36.
Tarasenko, Y. I., Yu, Y., Jordan, P. M., Bottenstein, J., & Wu, P. (2004). Effect of growth factors on proliferation and phenotypic differentiation of human fetal neural stem cells. Journal of Neuroscience Research, 78(5), 625-36.
Tarasenko YI, et al. Effect of Growth Factors On Proliferation and Phenotypic Differentiation of Human Fetal Neural Stem Cells. J Neurosci Res. 2004 Dec 1;78(5):625-36. PubMed PMID: 15490463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of growth factors on proliferation and phenotypic differentiation of human fetal neural stem cells. AU - Tarasenko,Yevgeniya I, AU - Yu,Yongjia, AU - Jordan,Paivi M, AU - Bottenstein,Jane, AU - Wu,Ping, PY - 2004/10/19/pubmed PY - 2005/3/1/medline PY - 2004/10/19/entrez SP - 625 EP - 36 JF - Journal of neuroscience research JO - J. Neurosci. Res. VL - 78 IS - 5 N2 - Human fetal neural stem cells (hNSCs) can be expanded in vitro by mitogens or growth factors, such as basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and/or leukemia inhibitory factor (LIF). Their effects on proliferation rate and differentiation pattern of hNSCs, however, have not been fully characterized. In this study, we cultured hNSCs in seven regimens, including bFGF, EGF, and LIF, either alone or in combinations. Cells were maintained as neurospheres in treatment media for various periods, up to six passages. A combination of bFGF, EGF, and LIF expanded hNSCs more efficiently than any other treatment as determined by counting total cell numbers using a trypan blue exclusion assay, a WST-1 cell viability assay, and a bromodeoxyuridine incorporation flow cytometric analysis. Differentiation patterns of hNSCs expanded under different conditions were also analyzed. We reported previously that hNSCs primed in vitro with a combination of bFGF, heparin, and laminin (FHL) induced neuronal differentiation toward a cholinergic phenotype. In this study, we show that the FHL priming increases neuronal differentiation while decreasing astroglial generation in all treatment groups as determined by immunostaining. However, cells proliferated under different growth factor conditions do vary in their phenotypic differentiation patterns. Particularly, significant generation of cholinergic cells was observed only in hNSCs expanded with EGF/bFGF or EGF/bFGF/LIF, but not with other treatment regimens, even when they are exposed to the same priming procedure. Our results indicate that hNSCs are highly plastic, with their proliferation and differentiation potential dependent on different growth factor treatments. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/15490463/Effect_of_growth_factors_on_proliferation_and_phenotypic_differentiation_of_human_fetal_neural_stem_cells_ L2 - https://doi.org/10.1002/jnr.20316 DB - PRIME DP - Unbound Medicine ER -