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Malaria diagnosis: false negative parasight-F tests in falciparum malaria patients in Nigeria.
Afr J Med Med Sci. 2004 Mar; 33(1):15-8.AJ

Abstract

This study was designed to assess the relative reliability of microscopy, the dipstick technique based on the detection of Plasmodium falciparum-specific histidine rich protein II (HRPII) (Parasight-F) and PCR assays in diagnosing falciparum malaria infections in Nigerian children. The prevalence of P. falciparum infections in enrolled patients was 100% by microscopy. Parasite density ranged from 329 to 81,194 parasites/microL of blood, with a geometric mean parasite density of 5168 parasites/microL of blood. The sensitivity of the HRPII based dipstick, PCR and microscopy were 80%, 92% and 100% respectively. A false negative rate of 20% was observed with Parasight-F as compared with microscopy. The parasitemia in patients with false negative Parasight-F tests ranged from 319 to 54,680 parasites/microL of blood. Detailed PCR analysis of Isolates obtained from five out of the eight patients who exhibited a negative Parasight-F test, showed that the average numbers of P. falciparum clones in these five isolates were: 1.7 +/- 1.02 with MSPI, 3.2 +/- 1.3 with MSP2 and 1.4 +/- 1.72 with GLURP. Comparison of microscopy and HRPII results showed a significant (p=0.009) difference as opposed to microscopy and PCR (p=0.239). This study showed that caution should be exercised when excluding P. falciparum infections on the basis of HRPII based dipstick results alone. Microscopy or PCR diagnosis where possible, should be carried out in order to confirm negative P. falciparum HRPII-based dipstick tests.

Authors+Show Affiliations

Happi CT
Malaria Research Laboratories, Postgraduate Institute for Medical Research and Training, College of Medicine, University of Ibadan, Nigeria. christianhappi@hotmail.com
Gbotosho GO
No affiliation info available
Sowunmi A
No affiliation info available
Falade CO
No affiliation info available
Akinboye DO
No affiliation info available
Oladepo O
No affiliation info available
Oduola AM
No affiliation info available

MeSH

AnimalsAntigens, ProtozoanChildChild, PreschoolDNA, ProtozoanFalse Negative ReactionsHumansInfantMalaria, FalciparumMerozoite Surface Protein 1MicroscopyNigeriaOutcome Assessment, Health CareParasitemiaPlasmodium falciparumPolymerase Chain ReactionPolymorphism, GeneticProteinsProtozoan ProteinsReagent Kits, DiagnosticReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15490787

Citation

Happi, C T., et al. "Malaria Diagnosis: False Negative parasight-F Tests in Falciparum Malaria Patients in Nigeria." African Journal of Medicine and Medical Sciences, vol. 33, no. 1, 2004, pp. 15-8.
Happi CT, Gbotosho GO, Sowunmi A, et al. Malaria diagnosis: false negative parasight-F tests in falciparum malaria patients in Nigeria. Afr J Med Med Sci. 2004;33(1):15-8.
Happi, C. T., Gbotosho, G. O., Sowunmi, A., Falade, C. O., Akinboye, D. O., Oladepo, O., & Oduola, A. M. (2004). Malaria diagnosis: false negative parasight-F tests in falciparum malaria patients in Nigeria. African Journal of Medicine and Medical Sciences, 33(1), 15-8.
Happi CT, et al. Malaria Diagnosis: False Negative parasight-F Tests in Falciparum Malaria Patients in Nigeria. Afr J Med Med Sci. 2004;33(1):15-8. PubMed PMID: 15490787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Malaria diagnosis: false negative parasight-F tests in falciparum malaria patients in Nigeria. AU - Happi,C T, AU - Gbotosho,G O, AU - Sowunmi,A, AU - Falade,C O, AU - Akinboye,D O, AU - Oladepo,O, AU - Oduola,A M J, PY - 2004/10/20/pubmed PY - 2005/4/6/medline PY - 2004/10/20/entrez SP - 15 EP - 8 JF - African journal of medicine and medical sciences JO - Afr J Med Med Sci VL - 33 IS - 1 N2 - This study was designed to assess the relative reliability of microscopy, the dipstick technique based on the detection of Plasmodium falciparum-specific histidine rich protein II (HRPII) (Parasight-F) and PCR assays in diagnosing falciparum malaria infections in Nigerian children. The prevalence of P. falciparum infections in enrolled patients was 100% by microscopy. Parasite density ranged from 329 to 81,194 parasites/microL of blood, with a geometric mean parasite density of 5168 parasites/microL of blood. The sensitivity of the HRPII based dipstick, PCR and microscopy were 80%, 92% and 100% respectively. A false negative rate of 20% was observed with Parasight-F as compared with microscopy. The parasitemia in patients with false negative Parasight-F tests ranged from 319 to 54,680 parasites/microL of blood. Detailed PCR analysis of Isolates obtained from five out of the eight patients who exhibited a negative Parasight-F test, showed that the average numbers of P. falciparum clones in these five isolates were: 1.7 +/- 1.02 with MSPI, 3.2 +/- 1.3 with MSP2 and 1.4 +/- 1.72 with GLURP. Comparison of microscopy and HRPII results showed a significant (p=0.009) difference as opposed to microscopy and PCR (p=0.239). This study showed that caution should be exercised when excluding P. falciparum infections on the basis of HRPII based dipstick results alone. Microscopy or PCR diagnosis where possible, should be carried out in order to confirm negative P. falciparum HRPII-based dipstick tests. SN - 0309-3913 UR - https://www.unboundmedicine.com/medline/citation/15490787/Malaria_diagnosis:_false_negative_parasight_F_tests_in_falciparum_malaria_patients_in_Nigeria_ DB - PRIME DP - Unbound Medicine ER -