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The response regulator SsrB activates transcription and binds to a region overlapping OmpR binding sites at Salmonella pathogenicity island 2.
Mol Microbiol. 2004 Nov; 54(3):823-35.MM

Abstract

OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth conditions indicate that expression of these two components is uncoupled, i.e. SsrB is produced in the absence of ssrA and vice versa. This result was suggested from our previous studies, in which two promoters at ssrA/B were identified. The isolated C-terminus of SsrB binds to DNA and protects regions upstream of ssrA, ssrB and srfH from DNase I digestion. Furthermore, the C-terminus of SsrB alone is capable of activating transcription in the absence of the N-terminus. Results from beta-galactosidase assays indicate that the N-terminal phosphorylation domain inhibits the C-terminal effector domain. A previous study from our laboratory reported that ssrA-lacZ and ssrB-lacZ transcriptional fusions were substantially reduced in an ssrB null strain. Results from DNase I protection assays provide direct evidence that SsrB binds at ssrA and ssrB, although the binding sites lie within the transcribed regions. Additional regulators clearly affect gene expression at this important locus, and here we provide evidence that SlyA, a transcription factor that contributes to Salmonella virulence, also affects ssrA/B gene expression.

Authors+Show Affiliations

Department of Microbiology and Immunology, University of Illinois at Chicago, 835 S. Wolcott Avenue, M/C 790, Chicago, IL 60612, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15491370

Citation

Feng, Xiuhong, et al. "The Response Regulator SsrB Activates Transcription and Binds to a Region Overlapping OmpR Binding Sites at Salmonella Pathogenicity Island 2." Molecular Microbiology, vol. 54, no. 3, 2004, pp. 823-35.
Feng X, Walthers D, Oropeza R, et al. The response regulator SsrB activates transcription and binds to a region overlapping OmpR binding sites at Salmonella pathogenicity island 2. Mol Microbiol. 2004;54(3):823-35.
Feng, X., Walthers, D., Oropeza, R., & Kenney, L. J. (2004). The response regulator SsrB activates transcription and binds to a region overlapping OmpR binding sites at Salmonella pathogenicity island 2. Molecular Microbiology, 54(3), 823-35.
Feng X, et al. The Response Regulator SsrB Activates Transcription and Binds to a Region Overlapping OmpR Binding Sites at Salmonella Pathogenicity Island 2. Mol Microbiol. 2004;54(3):823-35. PubMed PMID: 15491370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The response regulator SsrB activates transcription and binds to a region overlapping OmpR binding sites at Salmonella pathogenicity island 2. AU - Feng,Xiuhong, AU - Walthers,Don, AU - Oropeza,Ricardo, AU - Kenney,Linda J, PY - 2004/10/20/pubmed PY - 2005/7/7/medline PY - 2004/10/20/entrez SP - 823 EP - 35 JF - Molecular microbiology JO - Mol Microbiol VL - 54 IS - 3 N2 - OmpR activates expression of the two-component regulatory system located on Salmonella pathogenicity island 2 (SPI-2) that controls the expression of a type III secretion system, as well as many other genes required for systemic infection in mice. Measurements of SsrA and SsrB protein levels under different growth conditions indicate that expression of these two components is uncoupled, i.e. SsrB is produced in the absence of ssrA and vice versa. This result was suggested from our previous studies, in which two promoters at ssrA/B were identified. The isolated C-terminus of SsrB binds to DNA and protects regions upstream of ssrA, ssrB and srfH from DNase I digestion. Furthermore, the C-terminus of SsrB alone is capable of activating transcription in the absence of the N-terminus. Results from beta-galactosidase assays indicate that the N-terminal phosphorylation domain inhibits the C-terminal effector domain. A previous study from our laboratory reported that ssrA-lacZ and ssrB-lacZ transcriptional fusions were substantially reduced in an ssrB null strain. Results from DNase I protection assays provide direct evidence that SsrB binds at ssrA and ssrB, although the binding sites lie within the transcribed regions. Additional regulators clearly affect gene expression at this important locus, and here we provide evidence that SlyA, a transcription factor that contributes to Salmonella virulence, also affects ssrA/B gene expression. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/15491370/The_response_regulator_SsrB_activates_transcription_and_binds_to_a_region_overlapping_OmpR_binding_sites_at_Salmonella_pathogenicity_island_2_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2004&volume=54&issue=3&spage=823 DB - PRIME DP - Unbound Medicine ER -