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Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101.
Int J Mol Med. 2004 Nov; 14(5):867-72.IJ

Abstract

We have previously reported that human promyelocytic leukemia HL-60 cells can be induced to differentiate into mature granulocytes when HL-60 co-cultivated with human bone marrow stromal LP101 cells. In the present study, we investigated which factors produced by LP101 cells induce HL-60 cells to differentiate into mature granulocytes. The expression of the cell surface antigen CD11b on HL-60 cells was increased after a 72-h culture with the conditioned medium (CM) obtained from LP101 cells. LP101 cells were observed to produce various cytokines, including TNF-alpha, GM-CSF and IL-6. The neutralizing antibodies against these cytokines partially suppressed the CM-induced differentiation of HL-60 cells. Recombinant TNF-alpha induced the differentiation of HL-60 cells, and GM-CSF and IL-6 additionally enhanced the effect of TNF-alpha. When the CM was divided into a low molecular weight (LMW) fraction and a high molecular weight (HMW) fraction by ultrafiltration, the LMW fraction synergistically enhanced the differentiation inducible activity of TNF-alpha. These results demonstrate that LP101 cells induce the differentiation of HL-60 cells by producing various cytokines including TNF-alpha, IL-6, and GM-CSF, and that unknown low molecular weight factors also participate.

Authors+Show Affiliations

Department of Anatomy, Nihon University School of Medicine, Oyaguchikamicho, Itabashi-ku, Tokyo 173-8610, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15492858

Citation

Hiramoto, Masaki, et al. "Identification of Differentiation-inducing Activity Produced By Human Bone Marrow Stromal Cell Line LP101." International Journal of Molecular Medicine, vol. 14, no. 5, 2004, pp. 867-72.
Hiramoto M, Kawakami Y, Nabeshima R, et al. Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101. Int J Mol Med. 2004;14(5):867-72.
Hiramoto, M., Kawakami, Y., Nabeshima, R., Shima, D., Handa, H., & Aizawa, S. (2004). Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101. International Journal of Molecular Medicine, 14(5), 867-72.
Hiramoto M, et al. Identification of Differentiation-inducing Activity Produced By Human Bone Marrow Stromal Cell Line LP101. Int J Mol Med. 2004;14(5):867-72. PubMed PMID: 15492858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of differentiation-inducing activity produced by human bone marrow stromal cell line LP101. AU - Hiramoto,Masaki, AU - Kawakami,Yutaka, AU - Nabeshima,Ryusuke, AU - Shima,Daisuke, AU - Handa,Hiroshi, AU - Aizawa,Shin, PY - 2004/10/20/pubmed PY - 2005/6/1/medline PY - 2004/10/20/entrez SP - 867 EP - 72 JF - International journal of molecular medicine JO - Int J Mol Med VL - 14 IS - 5 N2 - We have previously reported that human promyelocytic leukemia HL-60 cells can be induced to differentiate into mature granulocytes when HL-60 co-cultivated with human bone marrow stromal LP101 cells. In the present study, we investigated which factors produced by LP101 cells induce HL-60 cells to differentiate into mature granulocytes. The expression of the cell surface antigen CD11b on HL-60 cells was increased after a 72-h culture with the conditioned medium (CM) obtained from LP101 cells. LP101 cells were observed to produce various cytokines, including TNF-alpha, GM-CSF and IL-6. The neutralizing antibodies against these cytokines partially suppressed the CM-induced differentiation of HL-60 cells. Recombinant TNF-alpha induced the differentiation of HL-60 cells, and GM-CSF and IL-6 additionally enhanced the effect of TNF-alpha. When the CM was divided into a low molecular weight (LMW) fraction and a high molecular weight (HMW) fraction by ultrafiltration, the LMW fraction synergistically enhanced the differentiation inducible activity of TNF-alpha. These results demonstrate that LP101 cells induce the differentiation of HL-60 cells by producing various cytokines including TNF-alpha, IL-6, and GM-CSF, and that unknown low molecular weight factors also participate. SN - 1107-3756 UR - https://www.unboundmedicine.com/medline/citation/15492858/Identification_of_differentiation_inducing_activity_produced_by_human_bone_marrow_stromal_cell_line_LP101_ DB - PRIME DP - Unbound Medicine ER -