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[Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats].
Yao Xue Xue Bao. 2004 Jul; 39(7):481-5.YX

Abstract

AIM

To investigate the effect of puerarin on expressions of MMP-2 and TIMP-2 in the kidney of diabetic rats.

METHODS

Uninephrectomized male Wistar rats were used to induce diabetes by intraperitoneal injection of streptozocin (65 mg x kg(-1)). Puerarin was given daily by intraperitoneal injection from the third day of induction of diabetes for 16 weeks. Using in situ hybridization and immunohistochemistry to detect MMP-2, TIMP-2 mRNA expressions and MMP-2, TIMP-2, collagen IV and Laminin expressions in diabetic kidneys with image analysis system, Flow cytometry was used to detect the expressions of TGFbeta1, MMP-2 and TIMP-2.

RESULTS

Compared with those in kidneys of control group, expressions of MMP-2 mRNA and proteins were lower, while the expressions of both TGFbeta1 and TIMP-2 were higher in the diabetic kidney (P < 0.05). The level of MMP-2 expression was advanced, while expression of TIMP-2 was reduced by puerarin treatment (P < 0.05).

CONCLUSION

Puerarin showed some renal protective effect on diabetic nephropathy, partly through inhibition of excessive deposition of glomeruli extracellular matrix by up-regulating MMP-2 and down-regulating TIMP-2 expressions besides reducing the blood glucose.

Authors+Show Affiliations

Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China. duanhj@hebmu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15493832

Citation

Duan, Hui-Jun, et al. "[Effects of Puerarin On Renal Function, Expressions of MMP-2 and TIMP-2 in Diabetic Rats]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 39, no. 7, 2004, pp. 481-5.
Duan HJ, Liu SX, Zhang YJ, et al. [Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats]. Yao Xue Xue Bao. 2004;39(7):481-5.
Duan, H. J., Liu, S. X., Zhang, Y. J., Liu, Q. J., He, N., & Li, Y. M. (2004). [Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 39(7), 481-5.
Duan HJ, et al. [Effects of Puerarin On Renal Function, Expressions of MMP-2 and TIMP-2 in Diabetic Rats]. Yao Xue Xue Bao. 2004;39(7):481-5. PubMed PMID: 15493832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of puerarin on renal function, expressions of MMP-2 and TIMP-2 in diabetic rats]. AU - Duan,Hui-Jun, AU - Liu,Shu-Xia, AU - Zhang,Yu-Jun, AU - Liu,Qing-Juan, AU - He,Ning, AU - Li,Ying-Min, PY - 2004/10/21/pubmed PY - 2005/1/4/medline PY - 2004/10/21/entrez SP - 481 EP - 5 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 39 IS - 7 N2 - AIM: To investigate the effect of puerarin on expressions of MMP-2 and TIMP-2 in the kidney of diabetic rats. METHODS: Uninephrectomized male Wistar rats were used to induce diabetes by intraperitoneal injection of streptozocin (65 mg x kg(-1)). Puerarin was given daily by intraperitoneal injection from the third day of induction of diabetes for 16 weeks. Using in situ hybridization and immunohistochemistry to detect MMP-2, TIMP-2 mRNA expressions and MMP-2, TIMP-2, collagen IV and Laminin expressions in diabetic kidneys with image analysis system, Flow cytometry was used to detect the expressions of TGFbeta1, MMP-2 and TIMP-2. RESULTS: Compared with those in kidneys of control group, expressions of MMP-2 mRNA and proteins were lower, while the expressions of both TGFbeta1 and TIMP-2 were higher in the diabetic kidney (P < 0.05). The level of MMP-2 expression was advanced, while expression of TIMP-2 was reduced by puerarin treatment (P < 0.05). CONCLUSION: Puerarin showed some renal protective effect on diabetic nephropathy, partly through inhibition of excessive deposition of glomeruli extracellular matrix by up-regulating MMP-2 and down-regulating TIMP-2 expressions besides reducing the blood glucose. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/15493832/[Effects_of_puerarin_on_renal_function_expressions_of_MMP_2_and_TIMP_2_in_diabetic_rats]_ L2 - https://medlineplus.gov/diabetickidneyproblems.html DB - PRIME DP - Unbound Medicine ER -