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Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes.
Yao Xue Xue Bao. 2004 Jul; 39(7):509-13.YX

Abstract

AIM

To elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na+/H+ exchanger inhibitor--amiloride.

METHODS

Single ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques.

RESULTS

Exposure to amiloride (10 -100 micromol x L(-1)), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak (Ca,L), But amiloride did not change the shape of their I - V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 micromol x L(-1) amiloride, I(Kr) was slightly depressed and I(Ks) did not change. Amiloride (1 - 100 micromol x L(-10) depressed I(K1) in a concentration-dependent manner.

CONCLUSION

Amiloride depressed potassium and calcium currents, which may give support to its uses in some diseases of the cardiovascular system.

Authors+Show Affiliations

Cheng L
Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. chenglan1@hotmail.com
Wang F
No affiliation info available
Zhou HY
No affiliation info available
Yao WX
No affiliation info available
Xia GJ
No affiliation info available
Jiang MX
No affiliation info available

MeSH

AmilorideAnimalsAnti-Arrhythmia AgentsCalcium Channels, L-TypeCalcium Channels, T-TypeCell SeparationFemaleGuinea PigsHeart VentriclesMaleMyocytes, CardiacPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedSodium-Hydrogen Exchangers

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15493838

Citation

Cheng, Lan, et al. "Effects of Amiloride On Potassium and Calcium Currents in Guinea Pig Ventricular Myocytes." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 39, no. 7, 2004, pp. 509-13.
Cheng L, Wang F, Zhou HY, et al. Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes. Yao Xue Xue Bao. 2004;39(7):509-13.
Cheng, L., Wang, F., Zhou, H. Y., Yao, W. X., Xia, G. J., & Jiang, M. X. (2004). Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 39(7), 509-13.
Cheng L, et al. Effects of Amiloride On Potassium and Calcium Currents in Guinea Pig Ventricular Myocytes. Yao Xue Xue Bao. 2004;39(7):509-13. PubMed PMID: 15493838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of amiloride on potassium and calcium currents in guinea pig ventricular myocytes. AU - Cheng,Lan, AU - Wang,Fang, AU - Zhou,Hong-Yi, AU - Yao,Wei-Xing, AU - Xia,Guo-Jin, AU - Jiang,Ming-Xing, PY - 2004/10/21/pubmed PY - 2005/1/4/medline PY - 2004/10/21/entrez SP - 509 EP - 13 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 39 IS - 7 N2 - AIM: To elucidate the possible mechanisms underlying antiarrhythmia of the non-selective Na+/H+ exchanger inhibitor--amiloride. METHODS: Single ventricular cells were isolated using a double-enzyme method. Effects of amiloride on voltage-dependent potassium and calcium currents in isolated guinea pig ventricular myocyte were recorded by using whole-cell patch clamp techniques. RESULTS: Exposure to amiloride (10 -100 micromol x L(-1)), the L-type and T-type calcium currents were depressed. Amiloride resulted in a concentration-dependent inhibition of peak (Ca,L), But amiloride did not change the shape of their I - V curves. It only decreased the amplitudes of the currents of the two types. When myocytes were incubated with 100 micromol x L(-1) amiloride, I(Kr) was slightly depressed and I(Ks) did not change. Amiloride (1 - 100 micromol x L(-10) depressed I(K1) in a concentration-dependent manner. CONCLUSION: Amiloride depressed potassium and calcium currents, which may give support to its uses in some diseases of the cardiovascular system. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/15493838/Effects_of_amiloride_on_potassium_and_calcium_currents_in_guinea_pig_ventricular_myocytes_ DB - PRIME DP - Unbound Medicine ER -