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Micellar electrokinetic chromatography with laser-induced fluorescence detection for rapid and sensitive analysis of two new bioactive reagents using dynamic covalent coating.
J Sep Sci. 2004 Sep; 27(13):1115-20.JS

Abstract

A simple, rapid, selective, and sensitive micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence detection (LIF) method was developed, using hexamethyldisilazane (HMDS) as dynamic covalent coating (DCC), for the analysis of two new bioactive agents N-n-hexyl-N'-(sodium p-aminobenzenesulfonate) thiourea (HXPT) and N-n-undecyl-N'-(sodium p-aminobenzenesulfonate) thiourea (UPT) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. MEKC methods both not using DCC and using DCC were investigated. In a series of optimization steps, DCC and a running buffer of 20 mM Na2B4O7 + 16 mM SDS + 8% acetonitrile were applied for determination of the derivatives. Linear relationships for HXPT and UPT were obtained in the range of 5 to 100 microM (correlation coefficient: 0.9986 for HXPT, 0.9978 for UPT), and the detection limits for HXPT and UPT were 16.5 and 39.0 ng mL(-1). The sensitivity was improved over that of fluorescence spectroscopy methods. The method was applied to the analysis of the two reagents in lab water waste with recoveries in the range of 95.6-107.5%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15495414

Citation

Xie, Jian Ping, et al. "Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Rapid and Sensitive Analysis of Two New Bioactive Reagents Using Dynamic Covalent Coating." Journal of Separation Science, vol. 27, no. 13, 2004, pp. 1115-20.
Xie JP, Cui FL, Chen XG, et al. Micellar electrokinetic chromatography with laser-induced fluorescence detection for rapid and sensitive analysis of two new bioactive reagents using dynamic covalent coating. J Sep Sci. 2004;27(13):1115-20.
Xie, J. P., Cui, F. L., Chen, X. G., & Hu, Z. D. (2004). Micellar electrokinetic chromatography with laser-induced fluorescence detection for rapid and sensitive analysis of two new bioactive reagents using dynamic covalent coating. Journal of Separation Science, 27(13), 1115-20.
Xie JP, et al. Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Rapid and Sensitive Analysis of Two New Bioactive Reagents Using Dynamic Covalent Coating. J Sep Sci. 2004;27(13):1115-20. PubMed PMID: 15495414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic chromatography with laser-induced fluorescence detection for rapid and sensitive analysis of two new bioactive reagents using dynamic covalent coating. AU - Xie,Jian Ping, AU - Cui,Feng Ling, AU - Chen,Xing Guo, AU - Hu,Zhi De, PY - 2004/10/22/pubmed PY - 2006/6/1/medline PY - 2004/10/22/entrez SP - 1115 EP - 20 JF - Journal of separation science JO - J Sep Sci VL - 27 IS - 13 N2 - A simple, rapid, selective, and sensitive micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence detection (LIF) method was developed, using hexamethyldisilazane (HMDS) as dynamic covalent coating (DCC), for the analysis of two new bioactive agents N-n-hexyl-N'-(sodium p-aminobenzenesulfonate) thiourea (HXPT) and N-n-undecyl-N'-(sodium p-aminobenzenesulfonate) thiourea (UPT) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. MEKC methods both not using DCC and using DCC were investigated. In a series of optimization steps, DCC and a running buffer of 20 mM Na2B4O7 + 16 mM SDS + 8% acetonitrile were applied for determination of the derivatives. Linear relationships for HXPT and UPT were obtained in the range of 5 to 100 microM (correlation coefficient: 0.9986 for HXPT, 0.9978 for UPT), and the detection limits for HXPT and UPT were 16.5 and 39.0 ng mL(-1). The sensitivity was improved over that of fluorescence spectroscopy methods. The method was applied to the analysis of the two reagents in lab water waste with recoveries in the range of 95.6-107.5%. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/15495414/Micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_for_rapid_and_sensitive_analysis_of_two_new_bioactive_reagents_using_dynamic_covalent_coating_ L2 - https://doi.org/10.1002/jssc.200401773 DB - PRIME DP - Unbound Medicine ER -