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[Pathological diagnosis and ultrastructure features of primary pulmonary cryptococcosis: a study of 27 cases].
Zhonghua Bing Li Xue Za Zhi. 2004 Oct; 33(5):424-8.ZB

Abstract

OBJECTIVE

To investigate diagnostic histopathology and ultrastructure features of primary pulmonary cryptococcosis (PC).

METHODS

Clinical data and pathologic findings of 27 cases of PC were retrospectively reviewed, light and electron microscopic evaluations and histochemistry stain studies were performed.

RESULTS

The specimens consisted of 2 fine-needle aspiration lung biopsies and 25 cases of open lung biopsies. Cryptococcosis granuloma formation was identifiable by histopathological examination in 25 of 27 cases, with gum-like lesion and fungi in the remaining 2 cases. The detection rates of cryptococcus neoformans (CN) by mucicarmine (MC), periodic acid-Schiff (PAS), alcian blue (AB) and Grocott methenamine-silver (GMS) were 87.0% (20/23), 100% (27/27), 66.7% (18/27), and 100% (23/23) respectively. Under the electron microscope, most CN had a simple structure with a few organelles. The capsule was seen in all organisms. A percentage of the organisms showed nuclei, nucleoli, mitochondria and vacuoles. The detection rate of CN by EM was 91.7% (11/12).

CONCLUSIONS

The clinical manifestation and imaging of PC are nonspecific for PC. Lung biopsy is the major diagnostic modality. The detection rate by electron microscopy was quite high. Therefore, a correct diagnosis of pulmonary cyrptococcosis should rely on the combination of histopathological evaluation, histochemistry staining and/or electron microscopic examination.

Authors+Show Affiliations

Yi XH
Department of Pathology, Shanghai Pulmonary Hospital, Shanghai 200433, China. yixhxf@yahoo.com.cn
Kong J
No affiliation info available
Zhu MF
No affiliation info available
Zhang Y
No affiliation info available
Chen XF
No affiliation info available
Zhong CS
No affiliation info available

MeSH

AdultAgedBiopsy, Fine-NeedleCryptococcosisCryptococcus neoformansDiagnosis, DifferentialFemaleFollow-Up StudiesHumansLungLung Diseases, FungalMaleMicroscopy, ElectronMiddle AgedRetrospective Studies

Pub Type(s)

Journal Article

Language

chi

PubMed ID

15498211

Citation

Yi, Xiang-hua, et al. "[Pathological Diagnosis and Ultrastructure Features of Primary Pulmonary Cryptococcosis: a Study of 27 Cases]." Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, vol. 33, no. 5, 2004, pp. 424-8.
Yi XH, Kong J, Zhu MF, et al. [Pathological diagnosis and ultrastructure features of primary pulmonary cryptococcosis: a study of 27 cases]. Zhonghua Bing Li Xue Za Zhi. 2004;33(5):424-8.
Yi, X. H., Kong, J., Zhu, M. F., Zhang, Y., Chen, X. F., & Zhong, C. S. (2004). [Pathological diagnosis and ultrastructure features of primary pulmonary cryptococcosis: a study of 27 cases]. Zhonghua Bing Li Xue Za Zhi = Chinese Journal of Pathology, 33(5), 424-8.
Yi XH, et al. [Pathological Diagnosis and Ultrastructure Features of Primary Pulmonary Cryptococcosis: a Study of 27 Cases]. Zhonghua Bing Li Xue Za Zhi. 2004;33(5):424-8. PubMed PMID: 15498211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Pathological diagnosis and ultrastructure features of primary pulmonary cryptococcosis: a study of 27 cases]. AU - Yi,Xiang-hua, AU - Kong,Jie, AU - Zhu,Mei-fang, AU - Zhang,Yun, AU - Chen,Xiao-feng, AU - Zhong,Ci-sheng, PY - 2004/10/23/pubmed PY - 2006/3/18/medline PY - 2004/10/23/entrez SP - 424 EP - 8 JF - Zhonghua bing li xue za zhi = Chinese journal of pathology JO - Zhonghua Bing Li Xue Za Zhi VL - 33 IS - 5 N2 - OBJECTIVE: To investigate diagnostic histopathology and ultrastructure features of primary pulmonary cryptococcosis (PC). METHODS: Clinical data and pathologic findings of 27 cases of PC were retrospectively reviewed, light and electron microscopic evaluations and histochemistry stain studies were performed. RESULTS: The specimens consisted of 2 fine-needle aspiration lung biopsies and 25 cases of open lung biopsies. Cryptococcosis granuloma formation was identifiable by histopathological examination in 25 of 27 cases, with gum-like lesion and fungi in the remaining 2 cases. The detection rates of cryptococcus neoformans (CN) by mucicarmine (MC), periodic acid-Schiff (PAS), alcian blue (AB) and Grocott methenamine-silver (GMS) were 87.0% (20/23), 100% (27/27), 66.7% (18/27), and 100% (23/23) respectively. Under the electron microscope, most CN had a simple structure with a few organelles. The capsule was seen in all organisms. A percentage of the organisms showed nuclei, nucleoli, mitochondria and vacuoles. The detection rate of CN by EM was 91.7% (11/12). CONCLUSIONS: The clinical manifestation and imaging of PC are nonspecific for PC. Lung biopsy is the major diagnostic modality. The detection rate by electron microscopy was quite high. Therefore, a correct diagnosis of pulmonary cyrptococcosis should rely on the combination of histopathological evaluation, histochemistry staining and/or electron microscopic examination. SN - 0529-5807 UR - https://www.unboundmedicine.com/medline/citation/15498211/[Pathological_diagnosis_and_ultrastructure_features_of_primary_pulmonary_cryptococcosis:_a_study_of_27_cases]_ DB - PRIME DP - Unbound Medicine ER -