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An in vitro study to compare 99mTc-stannous colloids and 99mTc-HMPAO for labelling human leukocytes.
Q J Nucl Med Mol Imaging. 2004 Sep; 48(3):229-36.QJ

Abstract

AIM

Aim of the present study was to compare in vitro the labelling efficiency (LE) and cell viability (TBE) of autologous leukocytes labelled with (99m)Tc-SnF(2) and (99m)Tc-HMPAO, and to evaluate the quantity and quality of spontaneously released (99m)Tc (SR) from labelled cells at several time points after labelling.

METHODS

A total of 14 patients with different diseases and 18 normal subjects were included in this study. A blood sample was collected from each patient; purified autologous leukocytes were divided into 2 samples and labelled with (99m)Tc-SnF(2) and (99m)Tc-HMPAO. LE was evaluated at the end of labelling and TBE and SR were evaluated at 10 min and 1 h, 2 h and 4 h after labelling.

RESULTS

LE of (99m)Tc-SnF(2)-WBC was higher than (99m)Tc-HMPAO-WBC (61.2+/-18.7% and 43.3+/-11.3; p<0.0001) and we found an inverse correlation between blood glucose and labelling efficiency for both methods (p=0.02). Minimal differences were also observed between 2 methods after 10 min and 1 h, as far as the cell viability is concerned. The percentage of radioactivity spontaneously released from (99m)Tc-SnF(2)-WBC was significantly higher compared to (99m)Tc-HMPAO-WBC at each time point. Radioactivity released from labelled cells was predominantly (99m)Tc-SnF(2) and (99m)Tc-HMPAO with few free (99m)Tc (<20%).

CONCLUSION

Both radiopharmaceuticals are not toxic for WBC. Labelling with (99m)Tc-SnF(2) give a higher LE than with (99m)Tc-HMPAO; however, radiolabelled colloids are more released from labelled cells over a period of 4 h. While (99m)Tc-HMPAO is physiological excreted into gastrointestinal tract, (99m)Tc-SnF(2) can be re-uptaken in vivo by reticulo-endothelial cells of liver and spleen. These findings suggest that (99m)Tc-SnF(2)-WBC might be better than (99m)Tc-HMPAO-WBC for studying inflammatory bowel diseases.

Authors+Show Affiliations

Capriotti G
Nuclear Medicine Unit, 2nd Faculty of Medicine, La Sapienza University, Rome, Italy.
D'Alessandria C
No affiliation info available
Garin E
No affiliation info available
Weber E
No affiliation info available
Devillers A
No affiliation info available
Lehmann K
No affiliation info available
Corsetti F
No affiliation info available
Lecloirec J
No affiliation info available
Meller J
No affiliation info available
Becker W
No affiliation info available
Moisan A
No affiliation info available
Signore A
No affiliation info available

MeSH

Cells, CulturedFemaleHumansIsotope LabelingLeukocytesMaleMiddle AgedRadiometryRadionuclide ImagingRadiopharmaceuticalsTechnetium CompoundsTechnetium Tc 99m ExametazimeTin Compounds

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15499297

Citation

Capriotti, G, et al. "An in Vitro Study to Compare 99mTc-stannous Colloids and 99mTc-HMPAO for Labelling Human Leukocytes." The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society Of..., vol. 48, no. 3, 2004, pp. 229-36.
Capriotti G, D'Alessandria C, Garin E, et al. An in vitro study to compare 99mTc-stannous colloids and 99mTc-HMPAO for labelling human leukocytes. Q J Nucl Med Mol Imaging. 2004;48(3):229-36.
Capriotti, G., D'Alessandria, C., Garin, E., Weber, E., Devillers, A., Lehmann, K., Corsetti, F., Lecloirec, J., Meller, J., Becker, W., Moisan, A., & Signore, A. (2004). An in vitro study to compare 99mTc-stannous colloids and 99mTc-HMPAO for labelling human leukocytes. The Quarterly Journal of Nuclear Medicine and Molecular Imaging : Official Publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society Of..., 48(3), 229-36.
Capriotti G, et al. An in Vitro Study to Compare 99mTc-stannous Colloids and 99mTc-HMPAO for Labelling Human Leukocytes. Q J Nucl Med Mol Imaging. 2004;48(3):229-36. PubMed PMID: 15499297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An in vitro study to compare 99mTc-stannous colloids and 99mTc-HMPAO for labelling human leukocytes. AU - Capriotti,G, AU - D'Alessandria,C, AU - Garin,E, AU - Weber,E, AU - Devillers,A, AU - Lehmann,K, AU - Corsetti,F, AU - Lecloirec,J, AU - Meller,J, AU - Becker,W, AU - Moisan,A, AU - Signore,A, PY - 2004/10/23/pubmed PY - 2005/2/16/medline PY - 2004/10/23/entrez SP - 229 EP - 36 JF - The quarterly journal of nuclear medicine and molecular imaging : official publication of the Italian Association of Nuclear Medicine (AIMN) [and] the International Association of Radiopharmacology (IAR), [and] Section of the Society of... JO - Q J Nucl Med Mol Imaging VL - 48 IS - 3 N2 - AIM: Aim of the present study was to compare in vitro the labelling efficiency (LE) and cell viability (TBE) of autologous leukocytes labelled with (99m)Tc-SnF(2) and (99m)Tc-HMPAO, and to evaluate the quantity and quality of spontaneously released (99m)Tc (SR) from labelled cells at several time points after labelling. METHODS: A total of 14 patients with different diseases and 18 normal subjects were included in this study. A blood sample was collected from each patient; purified autologous leukocytes were divided into 2 samples and labelled with (99m)Tc-SnF(2) and (99m)Tc-HMPAO. LE was evaluated at the end of labelling and TBE and SR were evaluated at 10 min and 1 h, 2 h and 4 h after labelling. RESULTS: LE of (99m)Tc-SnF(2)-WBC was higher than (99m)Tc-HMPAO-WBC (61.2+/-18.7% and 43.3+/-11.3; p<0.0001) and we found an inverse correlation between blood glucose and labelling efficiency for both methods (p=0.02). Minimal differences were also observed between 2 methods after 10 min and 1 h, as far as the cell viability is concerned. The percentage of radioactivity spontaneously released from (99m)Tc-SnF(2)-WBC was significantly higher compared to (99m)Tc-HMPAO-WBC at each time point. Radioactivity released from labelled cells was predominantly (99m)Tc-SnF(2) and (99m)Tc-HMPAO with few free (99m)Tc (<20%). CONCLUSION: Both radiopharmaceuticals are not toxic for WBC. Labelling with (99m)Tc-SnF(2) give a higher LE than with (99m)Tc-HMPAO; however, radiolabelled colloids are more released from labelled cells over a period of 4 h. While (99m)Tc-HMPAO is physiological excreted into gastrointestinal tract, (99m)Tc-SnF(2) can be re-uptaken in vivo by reticulo-endothelial cells of liver and spleen. These findings suggest that (99m)Tc-SnF(2)-WBC might be better than (99m)Tc-HMPAO-WBC for studying inflammatory bowel diseases. SN - 1824-4785 UR - https://www.unboundmedicine.com/medline/citation/15499297/An_in_vitro_study_to_compare_99mTc_stannous_colloids_and_99mTc_HMPAO_for_labelling_human_leukocytes_ DB - PRIME DP - Unbound Medicine ER -