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Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH.
Genome. 2004 Oct; 47(5):979-87.G

Abstract

Fluorescence in situ hybridization (FISH) is widely used in the physical mapping of genes and chromosome landmarks in plants and animals. Bacterial artificial chromosomes (BACs) contain large inserts, making them amenable for FISH mapping. In our BAC-FISH experiments, we selected 56 restriction fragment length polymorphism (RFLP)-locus-specific BAC clones from the libraries of Triticum monococcum and Aegilops tauschii, which are the A- and D-genome donors of wheat (Triticum aestivum, 2n = 6x = 42), respectively. The BAC clone 676D4 from the T. monococcum library contains a dispersed repeat that preferentially hybridizes to A-genome chromosomes, and two BAC clones, 9I10 and 9M13, from the Ae. tauschii library contain a dispersed repeat that preferentially hybridizes to the D-genome chromosomes. These repeats are useful in simultaneously discriminating the three different genomes in hexaploid wheat, and in identifying intergenomic translocations in wheat or between wheat and alien chromosomes. Sequencing results show that both of these repeats are transposable elements, indicating the importance of transposable elements, especially retrotransposons, in the genome evolution of wheat.

Authors+Show Affiliations

Wheat Genetics Resources Center, Department of Plant Pathology, 4024 Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15499412

Citation

Zhang, Peng, et al. "Simultaneous Painting of Three Genomes in Hexaploid Wheat By BAC-FISH." Genome, vol. 47, no. 5, 2004, pp. 979-87.
Zhang P, Li W, Friebe B, et al. Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome. 2004;47(5):979-87.
Zhang, P., Li, W., Friebe, B., & Gill, B. S. (2004). Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome, 47(5), 979-87.
Zhang P, et al. Simultaneous Painting of Three Genomes in Hexaploid Wheat By BAC-FISH. Genome. 2004;47(5):979-87. PubMed PMID: 15499412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. AU - Zhang,Peng, AU - Li,Wanlong, AU - Friebe,Bernd, AU - Gill,Bikram S, PY - 2004/10/23/pubmed PY - 2005/4/1/medline PY - 2004/10/23/entrez SP - 979 EP - 87 JF - Genome JO - Genome VL - 47 IS - 5 N2 - Fluorescence in situ hybridization (FISH) is widely used in the physical mapping of genes and chromosome landmarks in plants and animals. Bacterial artificial chromosomes (BACs) contain large inserts, making them amenable for FISH mapping. In our BAC-FISH experiments, we selected 56 restriction fragment length polymorphism (RFLP)-locus-specific BAC clones from the libraries of Triticum monococcum and Aegilops tauschii, which are the A- and D-genome donors of wheat (Triticum aestivum, 2n = 6x = 42), respectively. The BAC clone 676D4 from the T. monococcum library contains a dispersed repeat that preferentially hybridizes to A-genome chromosomes, and two BAC clones, 9I10 and 9M13, from the Ae. tauschii library contain a dispersed repeat that preferentially hybridizes to the D-genome chromosomes. These repeats are useful in simultaneously discriminating the three different genomes in hexaploid wheat, and in identifying intergenomic translocations in wheat or between wheat and alien chromosomes. Sequencing results show that both of these repeats are transposable elements, indicating the importance of transposable elements, especially retrotransposons, in the genome evolution of wheat. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/15499412/Simultaneous_painting_of_three_genomes_in_hexaploid_wheat_by_BAC_FISH_ L2 - https://cdnsciencepub.com/doi/10.1139/g04-042?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -