Tags

Type your tag names separated by a space and hit enter

Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea.
Exp Gerontol. 2004 Oct; 39(10):1537-43.EG

Abstract

The activities of superoxide dismutase, glutathione peroxidase (GPX) and catalase--the enzymatic scavengers of reactive oxygen species and the activities of xanthine oxidoreductase and xanthine oxidase, an enzyme known to generate reactive oxygen species, were studied in the corneas of normal rabbit eyes of various ages (1 month--young eyes; 4-9.5 months--young adult eyes; 2.0-2.75 years--middle aged eyes; 3.0-5.0 years--aged eyes). The activities of GPX, superoxide dismutase, xanthine oxidoreductase and xanthine oxidase were investigated biochemically in the scraped corneal epithelium. Catalase activity was detected histochemically in the corneal epithelium and endothelium. The results show that young corneas revealed lower activities of all the antioxidant enzymes investigated than did young adult corneas, in which enzymatic activities reached their maximum. In middle-aged corneas, GPX and catalase activities remained approximately at the same levels as seen in young adult corneas, whereas superoxide dismutase activity was decreased. In aged corneas, the activities of all antioxidant enzymes were dramatically decreased or even lost (catalase activity in the corneal endothelium). In contrast, xanthine oxidoreductase activity only slightly decreased with age and the xanthine oxidase proportion of total xanthine oxidoreductase remained unchanged. GPX, superoxide dismutase and catalase are important antioxidant enzymes protecting the cornea against the oxidative damage. Because the activities of these enzymes are lower in young animals and greatly reduced in aged animals, it is suggested that young and particularly aged corneas might be more susceptible to oxidative stress than are young adult corneas. This presumption is supported by the fact that the activities of prooxidant enzymes (xanthine oxidoreductase/xanthine oxidase) are only slightly decreased in aged corneas as compared to young adult corneas so that some imbalance between antioxidant and prooxidant enzymes exists already in the normal aged corneas.

Authors+Show Affiliations

Department of Eye Histochemistry, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Vídenská 1083, CZ-14220 Prague 4, Czech Republic. cejkova@biomed.cas.czNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15501024

Citation

Cejková, Jitka, et al. "Age-related Changes in Superoxide Dismutase, Glutathione Peroxidase, Catalase and Xanthine Oxidoreductase/xanthine Oxidase Activities in the Rabbit Cornea." Experimental Gerontology, vol. 39, no. 10, 2004, pp. 1537-43.
Cejková J, Vejrazka M, Pláteník J, et al. Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea. Exp Gerontol. 2004;39(10):1537-43.
Cejková, J., Vejrazka, M., Pláteník, J., & Stípek, S. (2004). Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea. Experimental Gerontology, 39(10), 1537-43.
Cejková J, et al. Age-related Changes in Superoxide Dismutase, Glutathione Peroxidase, Catalase and Xanthine Oxidoreductase/xanthine Oxidase Activities in the Rabbit Cornea. Exp Gerontol. 2004;39(10):1537-43. PubMed PMID: 15501024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Age-related changes in superoxide dismutase, glutathione peroxidase, catalase and xanthine oxidoreductase/xanthine oxidase activities in the rabbit cornea. AU - Cejková,Jitka, AU - Vejrazka,Martin, AU - Pláteník,Jan, AU - Stípek,Stanislav, PY - 2003/12/05/received PY - 2004/07/26/revised PY - 2004/08/13/accepted PY - 2004/10/27/pubmed PY - 2005/2/4/medline PY - 2004/10/27/entrez SP - 1537 EP - 43 JF - Experimental gerontology JO - Exp Gerontol VL - 39 IS - 10 N2 - The activities of superoxide dismutase, glutathione peroxidase (GPX) and catalase--the enzymatic scavengers of reactive oxygen species and the activities of xanthine oxidoreductase and xanthine oxidase, an enzyme known to generate reactive oxygen species, were studied in the corneas of normal rabbit eyes of various ages (1 month--young eyes; 4-9.5 months--young adult eyes; 2.0-2.75 years--middle aged eyes; 3.0-5.0 years--aged eyes). The activities of GPX, superoxide dismutase, xanthine oxidoreductase and xanthine oxidase were investigated biochemically in the scraped corneal epithelium. Catalase activity was detected histochemically in the corneal epithelium and endothelium. The results show that young corneas revealed lower activities of all the antioxidant enzymes investigated than did young adult corneas, in which enzymatic activities reached their maximum. In middle-aged corneas, GPX and catalase activities remained approximately at the same levels as seen in young adult corneas, whereas superoxide dismutase activity was decreased. In aged corneas, the activities of all antioxidant enzymes were dramatically decreased or even lost (catalase activity in the corneal endothelium). In contrast, xanthine oxidoreductase activity only slightly decreased with age and the xanthine oxidase proportion of total xanthine oxidoreductase remained unchanged. GPX, superoxide dismutase and catalase are important antioxidant enzymes protecting the cornea against the oxidative damage. Because the activities of these enzymes are lower in young animals and greatly reduced in aged animals, it is suggested that young and particularly aged corneas might be more susceptible to oxidative stress than are young adult corneas. This presumption is supported by the fact that the activities of prooxidant enzymes (xanthine oxidoreductase/xanthine oxidase) are only slightly decreased in aged corneas as compared to young adult corneas so that some imbalance between antioxidant and prooxidant enzymes exists already in the normal aged corneas. SN - 0531-5565 UR - https://www.unboundmedicine.com/medline/citation/15501024/Age_related_changes_in_superoxide_dismutase_glutathione_peroxidase_catalase_and_xanthine_oxidoreductase/xanthine_oxidase_activities_in_the_rabbit_cornea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0531-5565(04)00248-7 DB - PRIME DP - Unbound Medicine ER -