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Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment.
Acta Crystallogr D Biol Crystallogr. 2004 Nov; 60(Pt 11):2054-7.AC

Abstract

The Plasmodium vivax sexual stage 25 kDa protein Pvs25, located on the surface of the ookinete form of the parasite, is a vaccine candidate designed to elicit immunity that blocks the transmission of malaria by mosquitoes. The 2A8 murine monoclonal antibody directed against recombinant Pvs25 prevents the formation of P. vivax oocysts in mosquitoes fed in the laboratory. The complex between recombinant Pvs25 and the Fab fragment of 2A8 forms crystals that diffract X-rays to 3.5 A. Two native data sets, A and B, have been collected from crystals of the Pvs25-Fab complex. Both crystals belong to space group P2(1), with unit-cell parameters of a = 86.3, b = 61.7, c = 142.7 A, beta = 101.7 degrees for data set A and a = 86.8, b = 61.0, c = 149.3 A, beta = 104.3 degrees for data set B, and contain two complex molecules per asymmetric unit. Efforts are under way to reveal the structure of the Pvs25-Fab complex by molecular replacement. The three-dimensional structure of the Pvs25-Fab complex will provide an understanding of the interaction between Pvs25 and the 2A8 antibody that inhibits ookinete development in the mosquito and should aid in the development of transmission-blocking vaccines against P. vivax malaria.

Authors+Show Affiliations

Saxena AK
Structural Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Twinbrook II, 12441 Parklawn Drive, Rockville, Maryland 20852, USA.
Singh K
No affiliation info available
Long CA
No affiliation info available
Garboczi DN
No affiliation info available

MeSH

AnimalsAntibodies, ProtozoanAntigen-Antibody ComplexChromatography, GelCrystallizationCrystallography, X-RayImmunoglobulin Fab FragmentsMalaria, VivaxMicePlasmodium vivaxProtozoan Proteins

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15502325

Citation

Saxena, Ajay K., et al. "Preparation, Crystallization and Preliminary X-ray Analysis of a Complex Between the Plasmodium Vivax Sexual Stage 25 kDa Protein Pvs25 and a Malaria Transmission-blocking Antibody Fab Fragment." Acta Crystallographica. Section D, Biological Crystallography, vol. 60, no. Pt 11, 2004, pp. 2054-7.
Saxena AK, Singh K, Long CA, et al. Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 11):2054-7.
Saxena, A. K., Singh, K., Long, C. A., & Garboczi, D. N. (2004). Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment. Acta Crystallographica. Section D, Biological Crystallography, 60(Pt 11), 2054-7.
Saxena AK, et al. Preparation, Crystallization and Preliminary X-ray Analysis of a Complex Between the Plasmodium Vivax Sexual Stage 25 kDa Protein Pvs25 and a Malaria Transmission-blocking Antibody Fab Fragment. Acta Crystallogr D Biol Crystallogr. 2004;60(Pt 11):2054-7. PubMed PMID: 15502325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation, crystallization and preliminary X-ray analysis of a complex between the Plasmodium vivax sexual stage 25 kDa protein Pvs25 and a malaria transmission-blocking antibody Fab fragment. AU - Saxena,Ajay K, AU - Singh,Kavita, AU - Long,Carole A, AU - Garboczi,David N, Y1 - 2004/10/20/ PY - 2004/07/22/received PY - 2004/09/01/accepted PY - 2004/10/27/pubmed PY - 2005/5/17/medline PY - 2004/10/27/entrez SP - 2054 EP - 7 JF - Acta crystallographica. Section D, Biological crystallography JO - Acta Crystallogr D Biol Crystallogr VL - 60 IS - Pt 11 N2 - The Plasmodium vivax sexual stage 25 kDa protein Pvs25, located on the surface of the ookinete form of the parasite, is a vaccine candidate designed to elicit immunity that blocks the transmission of malaria by mosquitoes. The 2A8 murine monoclonal antibody directed against recombinant Pvs25 prevents the formation of P. vivax oocysts in mosquitoes fed in the laboratory. The complex between recombinant Pvs25 and the Fab fragment of 2A8 forms crystals that diffract X-rays to 3.5 A. Two native data sets, A and B, have been collected from crystals of the Pvs25-Fab complex. Both crystals belong to space group P2(1), with unit-cell parameters of a = 86.3, b = 61.7, c = 142.7 A, beta = 101.7 degrees for data set A and a = 86.8, b = 61.0, c = 149.3 A, beta = 104.3 degrees for data set B, and contain two complex molecules per asymmetric unit. Efforts are under way to reveal the structure of the Pvs25-Fab complex by molecular replacement. The three-dimensional structure of the Pvs25-Fab complex will provide an understanding of the interaction between Pvs25 and the 2A8 antibody that inhibits ookinete development in the mosquito and should aid in the development of transmission-blocking vaccines against P. vivax malaria. SN - 0907-4449 UR - https://www.unboundmedicine.com/medline/citation/15502325/Preparation_crystallization_and_preliminary_X_ray_analysis_of_a_complex_between_the_Plasmodium_vivax_sexual_stage_25_kDa_protein_Pvs25_and_a_malaria_transmission_blocking_antibody_Fab_fragment_ DB - PRIME DP - Unbound Medicine ER -
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