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Photoswitchable cyan fluorescent protein for protein tracking.
Nat Biotechnol. 2004 Nov; 22(11):1435-9.NBio

Abstract

In recent years diverse photolabeling techniques using green fluorescent protein (GFP)-like proteins have been reported, including photoactivatable PA-GFP, photoactivatable protein Kaede, the DsRed 'greening' technique and kindling fluorescent proteins. So far, only PA-GFP, which is monomeric and gives 100-fold fluorescence contrast, could be applied for protein tracking. Here we describe a dual-color monomeric protein, photoswitchable cyan fluorescent protein (PS-CFP). PS-CFP is capable of efficient photoconversion from cyan to green, changing both its excitation and emission spectra in response to 405-nm light irradiation. Complete photoactivation of PS-CFP results in a 1,500-fold increase in the green-to-cyan fluorescence ratio, making it the highest-contrast monomeric photoactivatable fluorescent protein described to date. We used PS-CFP as a photoswitchable tag to study trafficking of human dopamine transporter in living cells. At moderate excitation intensities, PS-CFP can be used as a pH-stable cyan label for protein tagging and fluorescence resonance energy transfer applications.

Authors+Show Affiliations

Institute of Bioorganic Chemistry RAS, Laboratory of Genes for Regeneration, Miklukho-Maklaya 16/10, Moscow 117997, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15502815

Citation

Chudakov, Dmitriy M., et al. "Photoswitchable Cyan Fluorescent Protein for Protein Tracking." Nature Biotechnology, vol. 22, no. 11, 2004, pp. 1435-9.
Chudakov DM, Verkhusha VV, Staroverov DB, et al. Photoswitchable cyan fluorescent protein for protein tracking. Nat Biotechnol. 2004;22(11):1435-9.
Chudakov, D. M., Verkhusha, V. V., Staroverov, D. B., Souslova, E. A., Lukyanov, S., & Lukyanov, K. A. (2004). Photoswitchable cyan fluorescent protein for protein tracking. Nature Biotechnology, 22(11), 1435-9.
Chudakov DM, et al. Photoswitchable Cyan Fluorescent Protein for Protein Tracking. Nat Biotechnol. 2004;22(11):1435-9. PubMed PMID: 15502815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photoswitchable cyan fluorescent protein for protein tracking. AU - Chudakov,Dmitriy M, AU - Verkhusha,Vladislav V, AU - Staroverov,Dmitry B, AU - Souslova,Ekaterina A, AU - Lukyanov,Sergey, AU - Lukyanov,Konstantin A, Y1 - 2004/10/24/ PY - 2004/03/05/received PY - 2004/08/16/accepted PY - 2004/10/27/pubmed PY - 2005/3/23/medline PY - 2004/10/27/entrez SP - 1435 EP - 9 JF - Nature biotechnology JO - Nat. Biotechnol. VL - 22 IS - 11 N2 - In recent years diverse photolabeling techniques using green fluorescent protein (GFP)-like proteins have been reported, including photoactivatable PA-GFP, photoactivatable protein Kaede, the DsRed 'greening' technique and kindling fluorescent proteins. So far, only PA-GFP, which is monomeric and gives 100-fold fluorescence contrast, could be applied for protein tracking. Here we describe a dual-color monomeric protein, photoswitchable cyan fluorescent protein (PS-CFP). PS-CFP is capable of efficient photoconversion from cyan to green, changing both its excitation and emission spectra in response to 405-nm light irradiation. Complete photoactivation of PS-CFP results in a 1,500-fold increase in the green-to-cyan fluorescence ratio, making it the highest-contrast monomeric photoactivatable fluorescent protein described to date. We used PS-CFP as a photoswitchable tag to study trafficking of human dopamine transporter in living cells. At moderate excitation intensities, PS-CFP can be used as a pH-stable cyan label for protein tagging and fluorescence resonance energy transfer applications. SN - 1087-0156 UR - https://www.unboundmedicine.com/medline/citation/15502815/Photoswitchable_cyan_fluorescent_protein_for_protein_tracking_ L2 - https://dx.doi.org/10.1038/nbt1025 DB - PRIME DP - Unbound Medicine ER -