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Involvement of C/EBP-alpha gene in in vitro activation of rat hepatic stellate cells.
Biochem Biophys Res Commun. 2004 Nov 26; 324(4):1309-18.BB

Abstract

Hepatic stellate cells (HSCs) play key roles in hepatic fibrosis. One of the most striking alterations in activated HSCs is loss of cytoplasmic lipid droplets. However, the association of lipid storage with the activation of HSCs remains unclear. CCAAT/enhancer-binding proteins family (C/EBPs), especially C/EBP-alpha, controls differentiation of adipocytes. We suggested that C/EBP-alpha gene may be involved in HSCs activation. The present results showed that the expression levels of C/EBP-alpha and C/EBP-beta genes declined in activated HSCs. Over-expression of C/EBP-alpha gene in activated HSCs: (1) inhibited HSCs proliferation, extracellular matrix-producing, alpha-smooth muscle actin gene expression, and induced rebound of cytoplasmic lipid droplets; (2) reduced retinoic acid receptor-beta, C/EBP-delta and -beta gene expressions, but increased the active form C/EBP-beta PSer(105), and induced retinoid X receptor-alpha gene expression; and (3) did not affect the protein level of p16INK4a, p21Cip1/WAF1 or p27Kip1. In conclusions, C/EBP-alpha gene is involved in in vitro activation of rat HSCs.

Authors+Show Affiliations

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15504357

Citation

Huang, Guang-Cun, et al. "Involvement of C/EBP-alpha Gene in in Vitro Activation of Rat Hepatic Stellate Cells." Biochemical and Biophysical Research Communications, vol. 324, no. 4, 2004, pp. 1309-18.
Huang GC, Zhang JS, Tang QQ. Involvement of C/EBP-alpha gene in in vitro activation of rat hepatic stellate cells. Biochem Biophys Res Commun. 2004;324(4):1309-18.
Huang, G. C., Zhang, J. S., & Tang, Q. Q. (2004). Involvement of C/EBP-alpha gene in in vitro activation of rat hepatic stellate cells. Biochemical and Biophysical Research Communications, 324(4), 1309-18.
Huang GC, Zhang JS, Tang QQ. Involvement of C/EBP-alpha Gene in in Vitro Activation of Rat Hepatic Stellate Cells. Biochem Biophys Res Commun. 2004 Nov 26;324(4):1309-18. PubMed PMID: 15504357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of C/EBP-alpha gene in in vitro activation of rat hepatic stellate cells. AU - Huang,Guang-Cun, AU - Zhang,Jin-Sheng, AU - Tang,Qi-Qun, PY - 2004/09/21/received PY - 2004/10/27/pubmed PY - 2005/2/8/medline PY - 2004/10/27/entrez SP - 1309 EP - 18 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 324 IS - 4 N2 - Hepatic stellate cells (HSCs) play key roles in hepatic fibrosis. One of the most striking alterations in activated HSCs is loss of cytoplasmic lipid droplets. However, the association of lipid storage with the activation of HSCs remains unclear. CCAAT/enhancer-binding proteins family (C/EBPs), especially C/EBP-alpha, controls differentiation of adipocytes. We suggested that C/EBP-alpha gene may be involved in HSCs activation. The present results showed that the expression levels of C/EBP-alpha and C/EBP-beta genes declined in activated HSCs. Over-expression of C/EBP-alpha gene in activated HSCs: (1) inhibited HSCs proliferation, extracellular matrix-producing, alpha-smooth muscle actin gene expression, and induced rebound of cytoplasmic lipid droplets; (2) reduced retinoic acid receptor-beta, C/EBP-delta and -beta gene expressions, but increased the active form C/EBP-beta PSer(105), and induced retinoid X receptor-alpha gene expression; and (3) did not affect the protein level of p16INK4a, p21Cip1/WAF1 or p27Kip1. In conclusions, C/EBP-alpha gene is involved in in vitro activation of rat HSCs. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15504357/Involvement_of_C/EBP_alpha_gene_in_in_vitro_activation_of_rat_hepatic_stellate_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(04)02257-0 DB - PRIME DP - Unbound Medicine ER -