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Anti-human versus anti-guinea pig tissue transglutaminase antibodies as the first-level serological screening test for coeliac disease in the general population.
Dig Liver Dis 2004; 36(10):671-6DL

Abstract

BACKGROUND

So far the reliability of the anti-guinea pig and anti-human tissue transglutaminase antibodies for the coeliac disease diagnosis has been evaluated in selected groups of patients.

AIM

To compare the diagnostic accuracy of anti-human versus anti-guinea pig tissue transglutaminase in the coeliac disease screening of the general population.

SUBJECTS

Two healthy Italian populations living in Marche region and in Western Sardinia.

METHODS

Both anti-guinea pig and anti-human tissue transglutaminase were determined using an enzyme-linked immunosorbent assay-based commercially available kit (Eu-tTG, Eurospital, Trieste, Italy).

RESULTS

During the period 1999-2001, 3541 subjects (1500 from "continental" Italy and 2041 from Sardinia) were screened for coeliac disease using both anti-guinea pig and anti-human tissue transglutaminase as first-level tests. Both these tests were negative in 3439/3541 sera, while 29 resulted positive for both of them and 73 showed discordant results. Overall, 50 intestinal biopsies were performed in 22, 21 and 7 subjects with positivity to both screening tests, to anti-guinea pig and to anti-human tissue transglutaminase alone, respectively. A coeliac disease diagnosis was made in 25 subjects giving an overall prevalence of 1:126 individuals. The anti-tissue transglutaminase specificity and sensitivity were 98 and 92% for guinea pig and 99.6 and 96% for human tissue transglutaminase, respectively.

CONCLUSIONS

The anti-human tissue transglutaminase test should definitely replace the anti-guinea pig-derived one as first-level screening tool for identifying all subjects who need the second-level investigations (small intestinal biopsy).

Authors+Show Affiliations

Department of Pediatrics, University of Ancona, Via F. Corridoni no. 11, 60123 Ancona, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15506666

Citation

Fabiani, E, et al. "Anti-human Versus Anti-guinea Pig Tissue Transglutaminase Antibodies as the First-level Serological Screening Test for Coeliac Disease in the General Population." Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, vol. 36, no. 10, 2004, pp. 671-6.
Fabiani E, Peruzzi E, Mandolesi A, et al. Anti-human versus anti-guinea pig tissue transglutaminase antibodies as the first-level serological screening test for coeliac disease in the general population. Dig Liver Dis. 2004;36(10):671-6.
Fabiani, E., Peruzzi, E., Mandolesi, A., Garbuglia, G., Fanciulli, G., D'Appello, A. R., ... Catassi, C. (2004). Anti-human versus anti-guinea pig tissue transglutaminase antibodies as the first-level serological screening test for coeliac disease in the general population. Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 36(10), pp. 671-6.
Fabiani E, et al. Anti-human Versus Anti-guinea Pig Tissue Transglutaminase Antibodies as the First-level Serological Screening Test for Coeliac Disease in the General Population. Dig Liver Dis. 2004;36(10):671-6. PubMed PMID: 15506666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-human versus anti-guinea pig tissue transglutaminase antibodies as the first-level serological screening test for coeliac disease in the general population. AU - Fabiani,E, AU - Peruzzi,E, AU - Mandolesi,A, AU - Garbuglia,G, AU - Fanciulli,G, AU - D'Appello,A R, AU - Gasparin,M, AU - Bravi,E, AU - Bearzi,I, AU - Galeazzi,R, AU - Catassi,C, PY - 2004/10/28/pubmed PY - 2005/2/11/medline PY - 2004/10/28/entrez SP - 671 EP - 6 JF - Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver JO - Dig Liver Dis VL - 36 IS - 10 N2 - BACKGROUND: So far the reliability of the anti-guinea pig and anti-human tissue transglutaminase antibodies for the coeliac disease diagnosis has been evaluated in selected groups of patients. AIM: To compare the diagnostic accuracy of anti-human versus anti-guinea pig tissue transglutaminase in the coeliac disease screening of the general population. SUBJECTS: Two healthy Italian populations living in Marche region and in Western Sardinia. METHODS: Both anti-guinea pig and anti-human tissue transglutaminase were determined using an enzyme-linked immunosorbent assay-based commercially available kit (Eu-tTG, Eurospital, Trieste, Italy). RESULTS: During the period 1999-2001, 3541 subjects (1500 from "continental" Italy and 2041 from Sardinia) were screened for coeliac disease using both anti-guinea pig and anti-human tissue transglutaminase as first-level tests. Both these tests were negative in 3439/3541 sera, while 29 resulted positive for both of them and 73 showed discordant results. Overall, 50 intestinal biopsies were performed in 22, 21 and 7 subjects with positivity to both screening tests, to anti-guinea pig and to anti-human tissue transglutaminase alone, respectively. A coeliac disease diagnosis was made in 25 subjects giving an overall prevalence of 1:126 individuals. The anti-tissue transglutaminase specificity and sensitivity were 98 and 92% for guinea pig and 99.6 and 96% for human tissue transglutaminase, respectively. CONCLUSIONS: The anti-human tissue transglutaminase test should definitely replace the anti-guinea pig-derived one as first-level screening tool for identifying all subjects who need the second-level investigations (small intestinal biopsy). SN - 1590-8658 UR - https://www.unboundmedicine.com/medline/citation/15506666/Anti_human_versus_anti_guinea_pig_tissue_transglutaminase_antibodies_as_the_first_level_serological_screening_test_for_coeliac_disease_in_the_general_population_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1590-8658(04)00262-2 DB - PRIME DP - Unbound Medicine ER -