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Identification of flavonoids in Hakmeitau beans (Vigna sinensis) by high-performance liquid chromatography-electrospray mass spectrometry (LC-ESI/MS).
J Agric Food Chem. 2004 Nov 03; 52(22):6694-9.JA

Abstract

Liquid chromatography coupled with electrospray mass spectrometry (LC-ESI/MS) with positive and negative ion detection was used for the identification of flavonoids in Hakmeitau beans, a black seed cultivar of cowpea (Vigna sinensis). Gradient elution with water and acetonitrile, both containing 2% formic acid, was employed in chromatographic separation. The peaks were identified by comparison of the retention times and the UV-vis spectroscopic and mass spectrometric data with authentic standards and/or literature data. The identified flavonoids included six anthocyanins (cyanidin 3-O-galactoside, cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, malvidin 3-O-glucoside, peonidin 3-O-glucoside, and petunidin 3-O-glucoside) and four flavonol/flavonol glycosides (kaempferol 3-O-glucoside, quercetin, quercetin 3-O-glucoside, and quercetin 3-O-6' '-acetylglucoside). The tentatively identified flavonoids included two anthocyanins (malvidin 3-O-acetylglucoside and peonidin 3-O-malonylglucoside) and three flavonol glycosides (myricetin-3-O-glucoside, quercetin 7-O-glucoside, and quercetin-3-O-diglucoside). These flavonoids are present in seed coats, and the contents of anthocyanins and flavonol glycosides were 20.7 and 2.0 mg/g, respectively.

Authors+Show Affiliations

Laboratory for Food Protein Production, Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, People's Republic of China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15506802

Citation

Chang, Qi, and Yum-Shing Wong. "Identification of Flavonoids in Hakmeitau Beans (Vigna Sinensis) By High-performance Liquid Chromatography-electrospray Mass Spectrometry (LC-ESI/MS)." Journal of Agricultural and Food Chemistry, vol. 52, no. 22, 2004, pp. 6694-9.
Chang Q, Wong YS. Identification of flavonoids in Hakmeitau beans (Vigna sinensis) by high-performance liquid chromatography-electrospray mass spectrometry (LC-ESI/MS). J Agric Food Chem. 2004;52(22):6694-9.
Chang, Q., & Wong, Y. S. (2004). Identification of flavonoids in Hakmeitau beans (Vigna sinensis) by high-performance liquid chromatography-electrospray mass spectrometry (LC-ESI/MS). Journal of Agricultural and Food Chemistry, 52(22), 6694-9.
Chang Q, Wong YS. Identification of Flavonoids in Hakmeitau Beans (Vigna Sinensis) By High-performance Liquid Chromatography-electrospray Mass Spectrometry (LC-ESI/MS). J Agric Food Chem. 2004 Nov 3;52(22):6694-9. PubMed PMID: 15506802.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of flavonoids in Hakmeitau beans (Vigna sinensis) by high-performance liquid chromatography-electrospray mass spectrometry (LC-ESI/MS). AU - Chang,Qi, AU - Wong,Yum-Shing, PY - 2004/10/28/pubmed PY - 2004/12/16/medline PY - 2004/10/28/entrez SP - 6694 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 22 N2 - Liquid chromatography coupled with electrospray mass spectrometry (LC-ESI/MS) with positive and negative ion detection was used for the identification of flavonoids in Hakmeitau beans, a black seed cultivar of cowpea (Vigna sinensis). Gradient elution with water and acetonitrile, both containing 2% formic acid, was employed in chromatographic separation. The peaks were identified by comparison of the retention times and the UV-vis spectroscopic and mass spectrometric data with authentic standards and/or literature data. The identified flavonoids included six anthocyanins (cyanidin 3-O-galactoside, cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, malvidin 3-O-glucoside, peonidin 3-O-glucoside, and petunidin 3-O-glucoside) and four flavonol/flavonol glycosides (kaempferol 3-O-glucoside, quercetin, quercetin 3-O-glucoside, and quercetin 3-O-6' '-acetylglucoside). The tentatively identified flavonoids included two anthocyanins (malvidin 3-O-acetylglucoside and peonidin 3-O-malonylglucoside) and three flavonol glycosides (myricetin-3-O-glucoside, quercetin 7-O-glucoside, and quercetin-3-O-diglucoside). These flavonoids are present in seed coats, and the contents of anthocyanins and flavonol glycosides were 20.7 and 2.0 mg/g, respectively. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15506802/Identification_of_flavonoids_in_Hakmeitau_beans__Vigna_sinensis__by_high_performance_liquid_chromatography_electrospray_mass_spectrometry__LC_ESI/MS__ L2 - https://doi.org/10.1021/jf049114a DB - PRIME DP - Unbound Medicine ER -