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Chemical fingerprinting of Andrographis paniculata using HPLC, HPTLC and densitometry.
Phytochem Anal. 2004 Sep-Oct; 15(5):280-5.PA

Abstract

An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug.

Authors+Show Affiliations

Srivastava A
Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow-226015, India.
Misra H
No affiliation info available
Verma RK
No affiliation info available
Gupta MM
No affiliation info available

MeSH

AndrographisChromatography, High Pressure LiquidChromatography, Thin LayerDiterpenesGlucosidesImage Processing, Computer-AssistedPlant ExtractsPlants, MedicinalTetrahydronaphthalenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15508831

Citation

Srivastava, Alpana, et al. "Chemical Fingerprinting of Andrographis Paniculata Using HPLC, HPTLC and Densitometry." Phytochemical Analysis : PCA, vol. 15, no. 5, 2004, pp. 280-5.
Srivastava A, Misra H, Verma RK, et al. Chemical fingerprinting of Andrographis paniculata using HPLC, HPTLC and densitometry. Phytochem Anal. 2004;15(5):280-5.
Srivastava, A., Misra, H., Verma, R. K., & Gupta, M. M. (2004). Chemical fingerprinting of Andrographis paniculata using HPLC, HPTLC and densitometry. Phytochemical Analysis : PCA, 15(5), 280-5.
Srivastava A, et al. Chemical Fingerprinting of Andrographis Paniculata Using HPLC, HPTLC and Densitometry. Phytochem Anal. 2004 Sep-Oct;15(5):280-5. PubMed PMID: 15508831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical fingerprinting of Andrographis paniculata using HPLC, HPTLC and densitometry. AU - Srivastava,Alpana, AU - Misra,Himanshu, AU - Verma,Ram K, AU - Gupta,Madan M, PY - 2004/10/29/pubmed PY - 2004/12/16/medline PY - 2004/10/29/entrez SP - 280 EP - 5 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 15 IS - 5 N2 - An attempt has been made to develop a method by which to determine the chemical fingerprint of Andrographis paniculata (Acanthaceae). High-performance thin layer chromatography (HPTLC) was used to analyse hexane, chloroform, methanol and water extracts of leaves of A. paniculata. A computerised densitometer was applied to the two-dimensional spectrographic image analysis of the HPTLC plates. An HPLC equipped with a photodiode array detector was used for the analyses of these different extracts. The analyses showed that andrographolide and neoandrographolide are absent in the hexane extract but are present in greater amounts in the methanol extract as compared with the other extracts. These chromatograms may serve as a chemical fingerprint of the drug A. paniculata for quality control purposes and in the preparation of formulations based on the drug. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/15508831/Chemical_fingerprinting_of_Andrographis_paniculata_using_HPLC_HPTLC_and_densitometry_ DB - PRIME DP - Unbound Medicine ER -