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Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle.
Reproduction. 2004 Nov; 128(5):545-53.R

Abstract

The actions of prolactin (PRL) on target cells depend on the type of prolactin receptor (PRLr) predominantly expressed, particularly whether the long PRLr isoform is expressed. The aims of this study were to determine the cellular localization and the changes in expression of long and short PRLr isoforms in sheep ovary throughout the estrous cycle. Long and short PRLrs were localized mostly in the same ovarian cells. Maximum signal intensity, particularly for long PRLrs, was found in stromal cells surrounding primordial and primary follicles, and, for both PRLrs, in granulosa cells of preantral follicles and in luteal cells. Moderate signal intensity for PRLrs was found in theca cells of preantral to ovulatory follicles, and in granulosa cells of antral follicles up to the gonadotropin-dependent stage. Decreasing immunoreactivity to PRLrs was found in granulosa cells of gonadotropin-dependent to ovulatory follicles. For long PRLrs in particular, no signal was found in mural granulosa cells of gonadotropin-dependent follicles; for both isoforms, no signal was found in most granulosa cells of ovulatory follicles. In primordial to gonadotropin-dependent follicles, cellular localization of PRLr was similar on days 0, 10 and 15 of the cycle. Oocytes consistently showed positive immunostaining for PRLrs. Comparative RT-PCR analysis of long and short PRLr expression showed that the short isoform is evenly expressed throughout the estrous cycle, whereas the expression of the long form increases at the time of estrus and decreases at mid-luteal phase and at the onset of the follicular phase. Expression of long PRLrs was greater than that of short PRLrs on day 0 of cycle; expression of both isoforms was similar on day 10 and on day 15, long PRLrs expression was lower than that of short PRLrs. Our results indicate that in sheep ovary, the maximum responsiveness to PRL might occur during the preovulatory phase of the estrous cycle.

Authors+Show Affiliations

Departamento de Fisiologí a Animal, Facultad de Veterinaria, Universidad Complutense, 28040-Madrid. rapicazo@vet.ucm.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15509700

Citation

Picazo, R A., et al. "Cellular Localization and Changes in Expression of Prolactin Receptor Isoforms in Sheep Ovary Throughout the Estrous Cycle." Reproduction (Cambridge, England), vol. 128, no. 5, 2004, pp. 545-53.
Picazo RA, García Ruiz JP, Santiago Moreno J, et al. Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle. Reproduction. 2004;128(5):545-53.
Picazo, R. A., García Ruiz, J. P., Santiago Moreno, J., González de Bulnes, A., Muñoz, J., Silván, G., Lorenzo, P. L., & Illera, J. C. (2004). Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle. Reproduction (Cambridge, England), 128(5), 545-53.
Picazo RA, et al. Cellular Localization and Changes in Expression of Prolactin Receptor Isoforms in Sheep Ovary Throughout the Estrous Cycle. Reproduction. 2004;128(5):545-53. PubMed PMID: 15509700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle. AU - Picazo,R A, AU - García Ruiz,J P, AU - Santiago Moreno,J, AU - González de Bulnes,A, AU - Muñoz,J, AU - Silván,G, AU - Lorenzo,P L, AU - Illera,J C, PY - 2004/10/29/pubmed PY - 2005/2/3/medline PY - 2004/10/29/entrez SP - 545 EP - 53 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 128 IS - 5 N2 - The actions of prolactin (PRL) on target cells depend on the type of prolactin receptor (PRLr) predominantly expressed, particularly whether the long PRLr isoform is expressed. The aims of this study were to determine the cellular localization and the changes in expression of long and short PRLr isoforms in sheep ovary throughout the estrous cycle. Long and short PRLrs were localized mostly in the same ovarian cells. Maximum signal intensity, particularly for long PRLrs, was found in stromal cells surrounding primordial and primary follicles, and, for both PRLrs, in granulosa cells of preantral follicles and in luteal cells. Moderate signal intensity for PRLrs was found in theca cells of preantral to ovulatory follicles, and in granulosa cells of antral follicles up to the gonadotropin-dependent stage. Decreasing immunoreactivity to PRLrs was found in granulosa cells of gonadotropin-dependent to ovulatory follicles. For long PRLrs in particular, no signal was found in mural granulosa cells of gonadotropin-dependent follicles; for both isoforms, no signal was found in most granulosa cells of ovulatory follicles. In primordial to gonadotropin-dependent follicles, cellular localization of PRLr was similar on days 0, 10 and 15 of the cycle. Oocytes consistently showed positive immunostaining for PRLrs. Comparative RT-PCR analysis of long and short PRLr expression showed that the short isoform is evenly expressed throughout the estrous cycle, whereas the expression of the long form increases at the time of estrus and decreases at mid-luteal phase and at the onset of the follicular phase. Expression of long PRLrs was greater than that of short PRLrs on day 0 of cycle; expression of both isoforms was similar on day 10 and on day 15, long PRLrs expression was lower than that of short PRLrs. Our results indicate that in sheep ovary, the maximum responsiveness to PRL might occur during the preovulatory phase of the estrous cycle. SN - 1470-1626 UR - https://www.unboundmedicine.com/medline/citation/15509700/Cellular_localization_and_changes_in_expression_of_prolactin_receptor_isoforms_in_sheep_ovary_throughout_the_estrous_cycle_ L2 - https://rep.bioscientifica.com/doi/10.1530/rep.1.00343 DB - PRIME DP - Unbound Medicine ER -