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Differential expression of estrogen receptors (ERalpha/ERbeta) in testis of mature and immature pigs.
Anat Rec A Discov Mol Cell Evol Biol. 2004 Dec; 281(2):1234-9.AR

Abstract

High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor alpha and estrogen receptor beta in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embedded-tissues using both mouse anti-human monoclonal IgG ERalpha and IgG ERbeta 1 isoform. Western blot analysis demonstrated antibody specificity. ERalpha staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the most Leydig cells of mature testes. ERbeta immunoreactivity was observed in spermatogonia and Leydig cells of immature gonads, while it was clearly detected in spermatogonia and in spermatocytes of adult pig testes. The differential ERalpha/ERbeta expression in germ and somatic cells of the gonads suggest a role of estrogens in function and in development of pig testis.

Authors+Show Affiliations

Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rendi, Cosenza, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15515162

Citation

Rago, Vittoria, et al. "Differential Expression of Estrogen Receptors (ERalpha/ERbeta) in Testis of Mature and Immature Pigs." The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology, vol. 281, no. 2, 2004, pp. 1234-9.
Rago V, Maggiolini M, Vivacqua A, et al. Differential expression of estrogen receptors (ERalpha/ERbeta) in testis of mature and immature pigs. Anat Rec A Discov Mol Cell Evol Biol. 2004;281(2):1234-9.
Rago, V., Maggiolini, M., Vivacqua, A., Palma, A., & Carpino, A. (2004). Differential expression of estrogen receptors (ERalpha/ERbeta) in testis of mature and immature pigs. The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology, 281(2), 1234-9.
Rago V, et al. Differential Expression of Estrogen Receptors (ERalpha/ERbeta) in Testis of Mature and Immature Pigs. Anat Rec A Discov Mol Cell Evol Biol. 2004;281(2):1234-9. PubMed PMID: 15515162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of estrogen receptors (ERalpha/ERbeta) in testis of mature and immature pigs. AU - Rago,Vittoria, AU - Maggiolini,Marcello, AU - Vivacqua,Adele, AU - Palma,Antonio, AU - Carpino,Amalia, PY - 2004/10/30/pubmed PY - 2005/3/18/medline PY - 2004/10/30/entrez SP - 1234 EP - 9 JF - The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology JO - Anat Rec A Discov Mol Cell Evol Biol VL - 281 IS - 2 N2 - High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor alpha and estrogen receptor beta in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embedded-tissues using both mouse anti-human monoclonal IgG ERalpha and IgG ERbeta 1 isoform. Western blot analysis demonstrated antibody specificity. ERalpha staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the most Leydig cells of mature testes. ERbeta immunoreactivity was observed in spermatogonia and Leydig cells of immature gonads, while it was clearly detected in spermatogonia and in spermatocytes of adult pig testes. The differential ERalpha/ERbeta expression in germ and somatic cells of the gonads suggest a role of estrogens in function and in development of pig testis. SN - 1552-4884 UR - https://www.unboundmedicine.com/medline/citation/15515162/Differential_expression_of_estrogen_receptors__ERalpha/ERbeta__in_testis_of_mature_and_immature_pigs_ L2 - https://doi.org/10.1002/ar.a.20131 DB - PRIME DP - Unbound Medicine ER -