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Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand.
J Med Assoc Thai. 2004 Sep; 87(9):1029-33.JM

Abstract

The occurrence and antimicrobial susceptibility of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae in patients attending Siriraj Hospital in Bangkok from August 2000 to January 2001 were determined. ESBL-producing isolates were screened with four different methods: disk diffusion according to the National Committee for Clinical Laboratory Standards (NCCLS) guidelines, Etest ESBL (CT/CTL and TZ/TZL), Oxoid combination discs and MIC Etest strip. Antimicrobial susceptibility testing were determined by a microdilution automatic method (VITEX system, bioMerieux). Of 22,178 clinical specimens, 400 (1.8%) K. pneumoniae were isolated Of 26% (104/400) of these isolates were suspected to be ESBL-producing. Rates of detection of ESBL-producing K. pneumoniae were 18.67%, 30% and 23.78% for blood, sputum and urine samples, respectively. Susceptibility testing has revealed that all 70 tested isolates including 53 isolates from blood and sputum and 17 isolates from urine samples were susceptible to imipenem (MIC< or =4 mg/L). None of the tested isolates were susceptible to cephalosporins, cephamycin and aztreonam. Rate of susceptibility to ciprofloxacin, levofloxacin, gentamicin and tobramycin were 60%, 64%, 28% and 9%, respectively, for isolates from blood and sputum; 71%, 71%, 18% and 6% for urinary isolates. The present findings revealed a high occurrence rate of multi-drug resistance ESBL-producing K. pneumoniae in patients attending the university hospital. Imipenem was highly active against ESBL-producing K. pneumoniae.

Authors+Show Affiliations

Department of Medical Sciences, Ministry of Public Health, Thailand. mayura@dmsc.moph.go.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15516002

Citation

Kusum, Mayura, et al. "Occurrence of Extended-spectrum Beta-lactamase in Clinical Isolates of Klebsiella Pneumoniae in a University Hospital, Thailand." Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, vol. 87, no. 9, 2004, pp. 1029-33.
Kusum M, Wongwanich S, Dhiraputra C, et al. Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand. J Med Assoc Thai. 2004;87(9):1029-33.
Kusum, M., Wongwanich, S., Dhiraputra, C., Pongpech, P., & Naenna, P. (2004). Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand. Journal of the Medical Association of Thailand = Chotmaihet Thangphaet, 87(9), 1029-33.
Kusum M, et al. Occurrence of Extended-spectrum Beta-lactamase in Clinical Isolates of Klebsiella Pneumoniae in a University Hospital, Thailand. J Med Assoc Thai. 2004;87(9):1029-33. PubMed PMID: 15516002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand. AU - Kusum,Mayura, AU - Wongwanich,Siripan, AU - Dhiraputra,Chirdsak, AU - Pongpech,Pintip, AU - Naenna,Penpun, PY - 2004/11/2/pubmed PY - 2004/12/16/medline PY - 2004/11/2/entrez SP - 1029 EP - 33 JF - Journal of the Medical Association of Thailand = Chotmaihet thangphaet JO - J Med Assoc Thai VL - 87 IS - 9 N2 - The occurrence and antimicrobial susceptibility of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae in patients attending Siriraj Hospital in Bangkok from August 2000 to January 2001 were determined. ESBL-producing isolates were screened with four different methods: disk diffusion according to the National Committee for Clinical Laboratory Standards (NCCLS) guidelines, Etest ESBL (CT/CTL and TZ/TZL), Oxoid combination discs and MIC Etest strip. Antimicrobial susceptibility testing were determined by a microdilution automatic method (VITEX system, bioMerieux). Of 22,178 clinical specimens, 400 (1.8%) K. pneumoniae were isolated Of 26% (104/400) of these isolates were suspected to be ESBL-producing. Rates of detection of ESBL-producing K. pneumoniae were 18.67%, 30% and 23.78% for blood, sputum and urine samples, respectively. Susceptibility testing has revealed that all 70 tested isolates including 53 isolates from blood and sputum and 17 isolates from urine samples were susceptible to imipenem (MIC< or =4 mg/L). None of the tested isolates were susceptible to cephalosporins, cephamycin and aztreonam. Rate of susceptibility to ciprofloxacin, levofloxacin, gentamicin and tobramycin were 60%, 64%, 28% and 9%, respectively, for isolates from blood and sputum; 71%, 71%, 18% and 6% for urinary isolates. The present findings revealed a high occurrence rate of multi-drug resistance ESBL-producing K. pneumoniae in patients attending the university hospital. Imipenem was highly active against ESBL-producing K. pneumoniae. SN - 0125-2208 UR - https://www.unboundmedicine.com/medline/citation/15516002/Occurrence_of_extended_spectrum_beta_lactamase_in_clinical_isolates_of_Klebsiella_pneumoniae_in_a_University_Hospital_Thailand_ L2 - https://www.diseaseinfosearch.org/result/4006 DB - PRIME DP - Unbound Medicine ER -