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Comparative evaluation of in-vitro effects of Brazilian green propolis and Baccharis dracunculifolia extracts on cariogenic factors of Streptococcus mutans.
Biol Pharm Bull 2004; 27(11):1834-9BP

Abstract

Streptococcus mutans triggers dental caries establishment by two major factors: synthesis of organic acids, which demineralize dental enamel, and synthesis of glucans, which mediate the attachment of bacteria to the tooth surface. Propolis is a natural product that may prevent dental caries. Baccharis dracunculifolia DC (Asteraceae), a native plant from Brazil, is the most important botanical origin for the production of green propolis (Brazilian propolis) by honeybees. However, whether B. dracunculifolia (Bd) has an anticariogenic effect, like green propolis, remains unknown. Herein, we have made a comparative evaluation of the effects of extracts from green propolis and Bd on the glucan synthesis and acidogenic potential of S. mutans. The inhibitory effects of the extracts on bacterial acid production were evaluated through the potentiometric measurement of pH from bacterial suspensions treated with serial concentrations of both extracts. Besides presenting close inhibitory values at the same concentration range, Bd leaf rinse and green propolis extracts had similar IC(50) values (0.41 and 0.34 mg/ml, respectively). Both extracts produced a bacteriostatic effect on S. mutans cultures at a concentration of 0.40 mg/ml. Estimated inhibitory values of green propolis and Bd leaf rinse extracts on the synthesis of insoluble glucans (IC(50)=12.9 and 25.0 microg/ml, respectively) and soluble glucans (IC(50)=50.4 and 49.1 microg/ml, respectively) were not significantly different from each other at p<0.05. The results demonstrate that Bd leaf rinse and green propolis extracts have similar inhibitory effects on the S. mutans cariogenic factors evaluated herein, and allowed us to suggest that Bd leaves may be a potential source for pharmaceutical products employed for this purpose.

Authors+Show Affiliations

Laboratório de Bioquímica, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-Universidade de São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15516733

Citation

Leitão, Denise Pimenta da Silva, et al. "Comparative Evaluation of In-vitro Effects of Brazilian Green Propolis and Baccharis Dracunculifolia Extracts On Cariogenic Factors of Streptococcus Mutans." Biological & Pharmaceutical Bulletin, vol. 27, no. 11, 2004, pp. 1834-9.
Leitão DP, Filho AA, Polizello AC, et al. Comparative evaluation of in-vitro effects of Brazilian green propolis and Baccharis dracunculifolia extracts on cariogenic factors of Streptococcus mutans. Biol Pharm Bull. 2004;27(11):1834-9.
Leitão, D. P., Filho, A. A., Polizello, A. C., Bastos, J. K., & Spadaro, A. C. (2004). Comparative evaluation of in-vitro effects of Brazilian green propolis and Baccharis dracunculifolia extracts on cariogenic factors of Streptococcus mutans. Biological & Pharmaceutical Bulletin, 27(11), pp. 1834-9.
Leitão DP, et al. Comparative Evaluation of In-vitro Effects of Brazilian Green Propolis and Baccharis Dracunculifolia Extracts On Cariogenic Factors of Streptococcus Mutans. Biol Pharm Bull. 2004;27(11):1834-9. PubMed PMID: 15516733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative evaluation of in-vitro effects of Brazilian green propolis and Baccharis dracunculifolia extracts on cariogenic factors of Streptococcus mutans. AU - Leitão,Denise Pimenta da Silva, AU - Filho,Ademar Alves da Silva, AU - Polizello,Ana Cristina Morseli, AU - Bastos,Jairo Kenupp, AU - Spadaro,Augusto César Cropanese, PY - 2004/11/2/pubmed PY - 2005/4/20/medline PY - 2004/11/2/entrez SP - 1834 EP - 9 JF - Biological & pharmaceutical bulletin JO - Biol. Pharm. Bull. VL - 27 IS - 11 N2 - Streptococcus mutans triggers dental caries establishment by two major factors: synthesis of organic acids, which demineralize dental enamel, and synthesis of glucans, which mediate the attachment of bacteria to the tooth surface. Propolis is a natural product that may prevent dental caries. Baccharis dracunculifolia DC (Asteraceae), a native plant from Brazil, is the most important botanical origin for the production of green propolis (Brazilian propolis) by honeybees. However, whether B. dracunculifolia (Bd) has an anticariogenic effect, like green propolis, remains unknown. Herein, we have made a comparative evaluation of the effects of extracts from green propolis and Bd on the glucan synthesis and acidogenic potential of S. mutans. The inhibitory effects of the extracts on bacterial acid production were evaluated through the potentiometric measurement of pH from bacterial suspensions treated with serial concentrations of both extracts. Besides presenting close inhibitory values at the same concentration range, Bd leaf rinse and green propolis extracts had similar IC(50) values (0.41 and 0.34 mg/ml, respectively). Both extracts produced a bacteriostatic effect on S. mutans cultures at a concentration of 0.40 mg/ml. Estimated inhibitory values of green propolis and Bd leaf rinse extracts on the synthesis of insoluble glucans (IC(50)=12.9 and 25.0 microg/ml, respectively) and soluble glucans (IC(50)=50.4 and 49.1 microg/ml, respectively) were not significantly different from each other at p<0.05. The results demonstrate that Bd leaf rinse and green propolis extracts have similar inhibitory effects on the S. mutans cariogenic factors evaluated herein, and allowed us to suggest that Bd leaves may be a potential source for pharmaceutical products employed for this purpose. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/15516733/Comparative_evaluation_of_in_vitro_effects_of_Brazilian_green_propolis_and_Baccharis_dracunculifolia_extracts_on_cariogenic_factors_of_Streptococcus_mutans_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/27.1834?from=PubMed DB - PRIME DP - Unbound Medicine ER -