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Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test.
Environ Mol Mutagen. 2004; 44(5):459-68.EM

Abstract

Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. In the present report, we have used the alkaline Comet assay and the micronucleus (MN) test to detect the genotoxicity due to multiple sources of pollution in the peripheral blood of two native estuarine fish (mullet and sea catfish) and evaluated possible interactive genotoxic effects from multiple contaminants and the seasonal variation of the genotoxicity. Mullet and sea catfish were captured in the Tramandai and Mampituba Rivers in the southern Brazilian state of Rio Grande do Sul. Reference animals were obtained from the Armazem lagoon. Fish captured in the two estuaries during the four seasons over a period of 2 years had increased levels of DNA damage and MN frequencies relative to the reference fish. In general, the alkaline Comet assay was more sensitive to the genotoxicity of the river contaminants than the MN test. The Comet assay demonstrated significant differences in fish captured at different seasons and at the two river sites, while the MN test showed significant differences only for the annual average for mullet from both sites and fish from the control site. The increases in DNA damage appear to be related to the increase in the number of people in the towns close to the study areas during the warm spring and summer seasons. Although no specific cause-effect relationships were established, comparison of the chemical contaminants and physical variations in the rivers with the genotoxicity data indicate that there may be some association between hydrocarbons, metals, pH, and water temperature and the level of damaged cells observed in mullet and sea catfish from the Tramandai and Mampituba estuaries.

Authors+Show Affiliations

Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. vmoraesdeandrade@yahoo.com.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15517567

Citation

Andrade, Vanessa Moraes de, et al. "Fish as Bioindicators to Assess the Effects of Pollution in Two Southern Brazilian Rivers Using the Comet Assay and Micronucleus Test." Environmental and Molecular Mutagenesis, vol. 44, no. 5, 2004, pp. 459-68.
Andrade VM, Silva Jd, Silva FR, et al. Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test. Environ Mol Mutagen. 2004;44(5):459-68.
Andrade, V. M., Silva, J. d., Silva, F. R., Heuser, V. D., Dias, J. F., Yoneama, M. L., & Freitas, T. R. (2004). Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test. Environmental and Molecular Mutagenesis, 44(5), 459-68.
Andrade VM, et al. Fish as Bioindicators to Assess the Effects of Pollution in Two Southern Brazilian Rivers Using the Comet Assay and Micronucleus Test. Environ Mol Mutagen. 2004;44(5):459-68. PubMed PMID: 15517567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test. AU - Andrade,Vanessa Moraes de, AU - Silva,Juliana da, AU - Silva,Fernanda R da, AU - Heuser,Vanina D, AU - Dias,Johnny F, AU - Yoneama,Maria Lúcia, AU - Freitas,Thales R O de, PY - 2004/11/2/pubmed PY - 2005/3/9/medline PY - 2004/11/2/entrez SP - 459 EP - 68 JF - Environmental and molecular mutagenesis JO - Environ Mol Mutagen VL - 44 IS - 5 N2 - Industrial effluents, agricultural runoff, and municipal wastewaters contain unknown substances and complex mixtures that are released into the environment and can lead to contamination of surface and subsurface waters. In the present report, we have used the alkaline Comet assay and the micronucleus (MN) test to detect the genotoxicity due to multiple sources of pollution in the peripheral blood of two native estuarine fish (mullet and sea catfish) and evaluated possible interactive genotoxic effects from multiple contaminants and the seasonal variation of the genotoxicity. Mullet and sea catfish were captured in the Tramandai and Mampituba Rivers in the southern Brazilian state of Rio Grande do Sul. Reference animals were obtained from the Armazem lagoon. Fish captured in the two estuaries during the four seasons over a period of 2 years had increased levels of DNA damage and MN frequencies relative to the reference fish. In general, the alkaline Comet assay was more sensitive to the genotoxicity of the river contaminants than the MN test. The Comet assay demonstrated significant differences in fish captured at different seasons and at the two river sites, while the MN test showed significant differences only for the annual average for mullet from both sites and fish from the control site. The increases in DNA damage appear to be related to the increase in the number of people in the towns close to the study areas during the warm spring and summer seasons. Although no specific cause-effect relationships were established, comparison of the chemical contaminants and physical variations in the rivers with the genotoxicity data indicate that there may be some association between hydrocarbons, metals, pH, and water temperature and the level of damaged cells observed in mullet and sea catfish from the Tramandai and Mampituba estuaries. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/15517567/Fish_as_bioindicators_to_assess_the_effects_of_pollution_in_two_southern_Brazilian_rivers_using_the_Comet_assay_and_micronucleus_test_ L2 - https://doi.org/10.1002/em.20070 DB - PRIME DP - Unbound Medicine ER -