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Molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from environmental and clinical specimens in a cardiac surgery intensive care unit.
Infect Control Hosp Epidemiol 2004; 25(10):852-5IC

Abstract

OBJECTIVES

To investigate environmental contamination by extended-spectrum beta-lactamase-producing Enterobacteriaceae and to perform a comparative molecular analysis of clinical and environmental strains.

SETTING

A 17-bed cardiac surgery intensive care unit of a 480-bed university teaching hospital.

METHODS

Following an outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae, an environmental survey revealed extensive contamination of the environment (particularly faucets, sink drains, and the joints of the countertops) by extended-spectrum beta-lactamase-producing Enterobacteriaceae. Environmental strains were compared with clinical strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA.

RESULTS

A total of 62 environmental strains belonging to 4 species of extended-spectrum beta-lactamase-producing Enterobacteriaceae were analyzed and compared with 43 clinical strains obtained from 34 patients. Comparative molecular analysis revealed 4 identical or closely related patterns (3 from Klebsiella oxytoca and 1 from Enterobacter cloacae) between environmental and clinical strains.

CONCLUSIONS

Moist surfaces may serve as sources of multiply resistant Enterobacteriaceae in the intensive care unit. Identification and disinfection of such sources may therefore be helpful in prevention and control of outbreaks.

Authors+Show Affiliations

Unité d'Hygiène Hospitalière, Hôpital Européen Georges Pompidou, 20-40 rue Leblanc, 75908 Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15518028

Citation

Kac, Guillaume, et al. "Molecular Epidemiology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Isolated From Environmental and Clinical Specimens in a Cardiac Surgery Intensive Care Unit." Infection Control and Hospital Epidemiology, vol. 25, no. 10, 2004, pp. 852-5.
Kac G, Podglajen I, Vaupré S, et al. Molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from environmental and clinical specimens in a cardiac surgery intensive care unit. Infect Control Hosp Epidemiol. 2004;25(10):852-5.
Kac, G., Podglajen, I., Vaupré, S., Colardelle, N., Buu-Hof, A., & Gutmann, L. (2004). Molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from environmental and clinical specimens in a cardiac surgery intensive care unit. Infection Control and Hospital Epidemiology, 25(10), pp. 852-5.
Kac G, et al. Molecular Epidemiology of Extended-spectrum Beta-lactamase-producing Enterobacteriaceae Isolated From Environmental and Clinical Specimens in a Cardiac Surgery Intensive Care Unit. Infect Control Hosp Epidemiol. 2004;25(10):852-5. PubMed PMID: 15518028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from environmental and clinical specimens in a cardiac surgery intensive care unit. AU - Kac,Guillaume, AU - Podglajen,Isabelle, AU - Vaupré,Sabine, AU - Colardelle,Nathalie, AU - Buu-Hof,Annie, AU - Gutmann,Laurent, PY - 2004/11/3/pubmed PY - 2005/2/8/medline PY - 2004/11/3/entrez SP - 852 EP - 5 JF - Infection control and hospital epidemiology JO - Infect Control Hosp Epidemiol VL - 25 IS - 10 N2 - OBJECTIVES: To investigate environmental contamination by extended-spectrum beta-lactamase-producing Enterobacteriaceae and to perform a comparative molecular analysis of clinical and environmental strains. SETTING: A 17-bed cardiac surgery intensive care unit of a 480-bed university teaching hospital. METHODS: Following an outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae, an environmental survey revealed extensive contamination of the environment (particularly faucets, sink drains, and the joints of the countertops) by extended-spectrum beta-lactamase-producing Enterobacteriaceae. Environmental strains were compared with clinical strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA. RESULTS: A total of 62 environmental strains belonging to 4 species of extended-spectrum beta-lactamase-producing Enterobacteriaceae were analyzed and compared with 43 clinical strains obtained from 34 patients. Comparative molecular analysis revealed 4 identical or closely related patterns (3 from Klebsiella oxytoca and 1 from Enterobacter cloacae) between environmental and clinical strains. CONCLUSIONS: Moist surfaces may serve as sources of multiply resistant Enterobacteriaceae in the intensive care unit. Identification and disinfection of such sources may therefore be helpful in prevention and control of outbreaks. SN - 0899-823X UR - https://www.unboundmedicine.com/medline/citation/15518028/Molecular_epidemiology_of_extended_spectrum_beta_lactamase_producing_Enterobacteriaceae_isolated_from_environmental_and_clinical_specimens_in_a_cardiac_surgery_intensive_care_unit_ L2 - https://www.cambridge.org/core/product/identifier/S0195941700080371/type/journal_article DB - PRIME DP - Unbound Medicine ER -