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A catalogue of selected derivatization reactions to label molecules in the subnanomolar or nanomolar range for CE and micro-HPLC with LIF detection.
J Capill Electrophor Microchip Technol. 2004 Jan-Apr; 9(1-2):1-11.JC

Abstract

Sensitivity is a major obstacle to biochemists studying complex biological mixtures. Laser-induced fluorescence (LIF) is a very sensitive method that can be used with micro-HPLC (microHPLC) or capillary electrophoresis studies and provides an effective solution to these problems. Derivatization is a very useful tool in LIF detection because it allows for the detection of many compounds and is selective, i.e., one dye will label one chemical function specifically. The major difficulty is choosing a derivatization reaction among the huge number of reactions described in the literature and making it work at a very low level of concentration. Another limitation is the use of a laser wavelength, which can excite the derivatized molecules. This article discusses selected derivatization reactions for labeling molecules in the subnanomolar or nanomolar range for CE and microHPLC. Derivatization for amines, sugars, aldehydes, carboxylic acids, thiols, diols, and nucleotides are described, and derivatization procedures are presented. In many cases, the subnanomolar and nanomolar concentration of these compounds can be detected using these chemical reactions.

Authors+Show Affiliations

Laboratoire des Interactions Moléculaire et Réactivité Chimique et Photochimique, Université Paul Sabatier, France. priollet@picometrics.comNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Validation Study

Language

eng

PubMed ID

15518289

Citation

Riollet, Pierre, and François Couderc. "A Catalogue of Selected Derivatization Reactions to Label Molecules in the Subnanomolar or Nanomolar Range for CE and micro-HPLC With LIF Detection." Journal of Capillary Electrophoresis and Microchip Technology, vol. 9, no. 1-2, 2004, pp. 1-11.
Riollet P, Couderc F. A catalogue of selected derivatization reactions to label molecules in the subnanomolar or nanomolar range for CE and micro-HPLC with LIF detection. J Capill Electrophor Microchip Technol. 2004;9(1-2):1-11.
Riollet, P., & Couderc, F. (2004). A catalogue of selected derivatization reactions to label molecules in the subnanomolar or nanomolar range for CE and micro-HPLC with LIF detection. Journal of Capillary Electrophoresis and Microchip Technology, 9(1-2), 1-11.
Riollet P, Couderc F. A Catalogue of Selected Derivatization Reactions to Label Molecules in the Subnanomolar or Nanomolar Range for CE and micro-HPLC With LIF Detection. J Capill Electrophor Microchip Technol. 2004 Jan-Apr;9(1-2):1-11. PubMed PMID: 15518289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A catalogue of selected derivatization reactions to label molecules in the subnanomolar or nanomolar range for CE and micro-HPLC with LIF detection. AU - Riollet,Pierre, AU - Couderc,François, PY - 2004/11/3/pubmed PY - 2005/3/9/medline PY - 2004/11/3/entrez SP - 1 EP - 11 JF - Journal of capillary electrophoresis and microchip technology JO - J Capill Electrophor Microchip Technol VL - 9 IS - 1-2 N2 - Sensitivity is a major obstacle to biochemists studying complex biological mixtures. Laser-induced fluorescence (LIF) is a very sensitive method that can be used with micro-HPLC (microHPLC) or capillary electrophoresis studies and provides an effective solution to these problems. Derivatization is a very useful tool in LIF detection because it allows for the detection of many compounds and is selective, i.e., one dye will label one chemical function specifically. The major difficulty is choosing a derivatization reaction among the huge number of reactions described in the literature and making it work at a very low level of concentration. Another limitation is the use of a laser wavelength, which can excite the derivatized molecules. This article discusses selected derivatization reactions for labeling molecules in the subnanomolar or nanomolar range for CE and microHPLC. Derivatization for amines, sugars, aldehydes, carboxylic acids, thiols, diols, and nucleotides are described, and derivatization procedures are presented. In many cases, the subnanomolar and nanomolar concentration of these compounds can be detected using these chemical reactions. SN - 1946-4940 UR - https://www.unboundmedicine.com/medline/citation/15518289/A_catalogue_of_selected_derivatization_reactions_to_label_molecules_in_the_subnanomolar_or_nanomolar_range_for_CE_and_micro_HPLC_with_LIF_detection_ DB - PRIME DP - Unbound Medicine ER -