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Regulation of high glucose-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase.
Biochem Biophys Res Commun. 2004 Dec 03; 325(1):32-8.BB

Abstract

A high concentration of glucose has been implicated as a causal factor in initiation and progression of diabetic kidney complications, and there is evidence to suggest that hyperglycemia increases the production of free radicals and oxidant stress. Recently, we demonstrated that the control of mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) to supply NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPm activity in HEK293 cells, an embryonic kidney cell line, regulates high glucose-induced apoptosis. When we examined the protective role of IDPm against high glucose-induced apoptosis with HEK293 cells transfected with the cDNA for mouse IDPm in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPm expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPm plays an important protective role in apoptosis of HEK293 cells induced by a high concentration of glucose and may contribute to various pathologies associated with the long-term complications of diabetes.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15522197

Citation

Shin, Ai Hyang, et al. "Regulation of High Glucose-induced Apoptosis By Mitochondrial NADP+-dependent Isocitrate Dehydrogenase." Biochemical and Biophysical Research Communications, vol. 325, no. 1, 2004, pp. 32-8.
Shin AH, Kil IS, Yang ES, et al. Regulation of high glucose-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. Biochem Biophys Res Commun. 2004;325(1):32-8.
Shin, A. H., Kil, I. S., Yang, E. S., Huh, T. L., Yang, C. H., & Park, J. W. (2004). Regulation of high glucose-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. Biochemical and Biophysical Research Communications, 325(1), 32-8.
Shin AH, et al. Regulation of High Glucose-induced Apoptosis By Mitochondrial NADP+-dependent Isocitrate Dehydrogenase. Biochem Biophys Res Commun. 2004 Dec 3;325(1):32-8. PubMed PMID: 15522197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of high glucose-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. AU - Shin,Ai Hyang, AU - Kil,In Sup, AU - Yang,Eun Sun, AU - Huh,Tae Lin, AU - Yang,Chae Ha, AU - Park,Jeen-Woo, PY - 2004/09/20/received PY - 2004/11/4/pubmed PY - 2005/1/26/medline PY - 2004/11/4/entrez SP - 32 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 325 IS - 1 N2 - A high concentration of glucose has been implicated as a causal factor in initiation and progression of diabetic kidney complications, and there is evidence to suggest that hyperglycemia increases the production of free radicals and oxidant stress. Recently, we demonstrated that the control of mitochondrial redox balance and the cellular defense against oxidative damage is one of the primary functions of mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) to supply NADPH for antioxidant systems. In this report, we demonstrate that modulation of IDPm activity in HEK293 cells, an embryonic kidney cell line, regulates high glucose-induced apoptosis. When we examined the protective role of IDPm against high glucose-induced apoptosis with HEK293 cells transfected with the cDNA for mouse IDPm in sense and antisense orientations, a clear inverse relationship was observed between the amount of IDPm expressed in target cells and their susceptibility to apoptosis. The results suggest that IDPm plays an important protective role in apoptosis of HEK293 cells induced by a high concentration of glucose and may contribute to various pathologies associated with the long-term complications of diabetes. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15522197/Regulation_of_high_glucose_induced_apoptosis_by_mitochondrial_NADP+_dependent_isocitrate_dehydrogenase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(04)02247-8 DB - PRIME DP - Unbound Medicine ER -