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Mapping regional oxygenation and flow in pig hearts in vivo using near-infrared spectroscopic imaging.
J Mol Cell Cardiol 2004; 37(5):947-57JM

Abstract

We used near-infrared spectroscopic (NIRS) imaging to assess the flow-dependence of both myocardial oxygenation and distribution of an intravascular tracer, indocyanine green (ICG). In open-chest pigs, nominal flow through the left anterior descending artery was reduced for 90 to 0 min (n = 6), 20 +/- 1 (n = 5) and 44% +/- 5% (n = 4) by variable occlusion and subsequently restored (to 219% +/- 71% at 45 min) for 120 min. Electrocardiogram-gated NIRS images of the heart were obtained using a CCD-array camera with a liquid crystal tunable filter, which acquired absorbance spectra in the range of 650-1050 nm for each of 256 x 256 pixels (0.4 x 0.4 mm each). Deoxy- and oxy-(hemoglobin (Hb) + myoglobin (Mb)) levels were determined independently by applying a spectral fitting algorithm to the spectra between 650 and 890 nm. Maps for oxygenation parameter (OP = oxy-(Hb + Mb)/deoxy-(Hb + Mb)) and oxygen saturation parameter (OSP) (oxy-(Hb + Mb)/total-(Hb + Mb)) were constructed. To visualize flow distribution, a bolus of ICG (8.3 mg/5 ml) was injected I.V. at each step of the protocol and gated images were acquired at 800 nm every second over 60 s period. The ratio of ICG wash-in velocity to equilibrium absorbance (V/DeltaA(tail)) was calculated. Changes in flow to 0%, 20%, 44%, 100% and 208% of baseline resulted in OP values of 1.46 +/- 0.25, 1.66 +/- 0.30, 2.22 +/- 0.45, 2.78 +/- 0.30 and 3.94 +/- 0.33 in the affected area. Flow rates of 20%, 44%, 100% and 220% of baseline corresponded to V/DeltaA(tail) values of 0.11 +/- 0.11, 0.39 +/- 0.13, 0.54 +/- 0.17 and 0.61 +/- 0.20, respectively. Thus, measurements of oxygenation and normalized ICG wash-in velocity correlated well with coronary flow, allowing for intraoperative optical assessment of the severity of regional ischemia.

Authors+Show Affiliations

Institute for Biodiagnostics, National Research Council of Canada, 435 Ellice Avenue, Winnipeg, MB, Canada R3B 1Y6. valery.kupriyanov@nrc-cnrc.gc.caNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15522272

Citation

Kupriyanov, Valery V., et al. "Mapping Regional Oxygenation and Flow in Pig Hearts in Vivo Using Near-infrared Spectroscopic Imaging." Journal of Molecular and Cellular Cardiology, vol. 37, no. 5, 2004, pp. 947-57.
Kupriyanov VV, Nighswander-Rempel S, Xiang B. Mapping regional oxygenation and flow in pig hearts in vivo using near-infrared spectroscopic imaging. J Mol Cell Cardiol. 2004;37(5):947-57.
Kupriyanov, V. V., Nighswander-Rempel, S., & Xiang, B. (2004). Mapping regional oxygenation and flow in pig hearts in vivo using near-infrared spectroscopic imaging. Journal of Molecular and Cellular Cardiology, 37(5), pp. 947-57.
Kupriyanov VV, Nighswander-Rempel S, Xiang B. Mapping Regional Oxygenation and Flow in Pig Hearts in Vivo Using Near-infrared Spectroscopic Imaging. J Mol Cell Cardiol. 2004;37(5):947-57. PubMed PMID: 15522272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping regional oxygenation and flow in pig hearts in vivo using near-infrared spectroscopic imaging. AU - Kupriyanov,Valery V, AU - Nighswander-Rempel,Stephen, AU - Xiang,Bo, PY - 2004/04/16/received PY - 2004/06/01/revised PY - 2004/07/07/accepted PY - 2004/11/4/pubmed PY - 2005/3/26/medline PY - 2004/11/4/entrez SP - 947 EP - 57 JF - Journal of molecular and cellular cardiology JO - J. Mol. Cell. Cardiol. VL - 37 IS - 5 N2 - We used near-infrared spectroscopic (NIRS) imaging to assess the flow-dependence of both myocardial oxygenation and distribution of an intravascular tracer, indocyanine green (ICG). In open-chest pigs, nominal flow through the left anterior descending artery was reduced for 90 to 0 min (n = 6), 20 +/- 1 (n = 5) and 44% +/- 5% (n = 4) by variable occlusion and subsequently restored (to 219% +/- 71% at 45 min) for 120 min. Electrocardiogram-gated NIRS images of the heart were obtained using a CCD-array camera with a liquid crystal tunable filter, which acquired absorbance spectra in the range of 650-1050 nm for each of 256 x 256 pixels (0.4 x 0.4 mm each). Deoxy- and oxy-(hemoglobin (Hb) + myoglobin (Mb)) levels were determined independently by applying a spectral fitting algorithm to the spectra between 650 and 890 nm. Maps for oxygenation parameter (OP = oxy-(Hb + Mb)/deoxy-(Hb + Mb)) and oxygen saturation parameter (OSP) (oxy-(Hb + Mb)/total-(Hb + Mb)) were constructed. To visualize flow distribution, a bolus of ICG (8.3 mg/5 ml) was injected I.V. at each step of the protocol and gated images were acquired at 800 nm every second over 60 s period. The ratio of ICG wash-in velocity to equilibrium absorbance (V/DeltaA(tail)) was calculated. Changes in flow to 0%, 20%, 44%, 100% and 208% of baseline resulted in OP values of 1.46 +/- 0.25, 1.66 +/- 0.30, 2.22 +/- 0.45, 2.78 +/- 0.30 and 3.94 +/- 0.33 in the affected area. Flow rates of 20%, 44%, 100% and 220% of baseline corresponded to V/DeltaA(tail) values of 0.11 +/- 0.11, 0.39 +/- 0.13, 0.54 +/- 0.17 and 0.61 +/- 0.20, respectively. Thus, measurements of oxygenation and normalized ICG wash-in velocity correlated well with coronary flow, allowing for intraoperative optical assessment of the severity of regional ischemia. SN - 0022-2828 UR - https://www.unboundmedicine.com/medline/citation/15522272/Mapping_regional_oxygenation_and_flow_in_pig_hearts_in_vivo_using_near_infrared_spectroscopic_imaging_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2828(04)00232-9 DB - PRIME DP - Unbound Medicine ER -