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Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70.
J Surg Res. 2004 Nov; 122(1):96-102.JS

Abstract

BACKGROUND

Reperfusion of the liver after ischemia induces the expression of the heat shock genes and the synthesis of the heat shock proteins (HSP). We studied the effects of the natural antioxidant ergothioneine (EGT) treatment on the expression of HSP70 in ischemic-reperfused (IR) liver.

METHODS

Adult male Wistar rats were randomly divided into three groups: Sham group given standard laboratory chow and water for 3 weeks followed by sham operation; Control group given standard laboratory chow and water for 3 weeks followed by liver IR injury; EGT group given standard laboratory chow supplementation l-ergothioneine (1.2 mg/kg/d body weight) administered by gavage and water for 3 weeks followed by liver IR injury. Ten rats from each group were killed to determine serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactic dehydrogenase (LDH), tissue malondialdehyde (MDA), HSP70 levels, and histologic changes at 30, 60, and 120 min of reperfusion, respectively. Survival was followed for 1 week.

RESULTS

IR caused significant increase in serum AST, ALT, LDH, and tissue MDA levels. As compared with the control group, animals treated with EGT experienced a significant decrease in serum AST, ALT, and LDH levels in all reperfusion periods. Tissue MDA levels in animals receiving EGT were significantly reduced as compared with control group at 30 min and 60 min after reperfusion. After ischemia, reperfusion caused a remarkable production of HSP70 in the control group. When the rats were pretreated with EGT, the levels of HSP70 increased significantly in their livers after reperfusion compared with the control group. Liver injury in the EGT-treated animals was lower to that in the control group. The 7-day survival rate was significantly improved (from 50% to 80%) by EGT pretreatment.

CONCLUSION

HSP70 has been shown to induce tolerance against warm IR injury in rat livers. EGT pretreatment protects the liver from IR injury by over-expression of HSP and the subsequent suppression of lipid peroxidation.

Authors+Show Affiliations

Department of General Surgery, University of Gazi, School of Medicine, Ankara, Turkey. bedirlia@gazi.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15522321

Citation

Bedirli, Abdulkadir, et al. "Ergothioneine Pretreatment Protects the Liver From Ischemia-reperfusion Injury Caused By Increasing Hepatic Heat Shock Protein 70." The Journal of Surgical Research, vol. 122, no. 1, 2004, pp. 96-102.
Bedirli A, Sakrak O, Muhtaroglu S, et al. Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70. J Surg Res. 2004;122(1):96-102.
Bedirli, A., Sakrak, O., Muhtaroglu, S., Soyuer, I., Guler, I., Riza Erdogan, A., & Sozuer, E. M. (2004). Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70. The Journal of Surgical Research, 122(1), 96-102.
Bedirli A, et al. Ergothioneine Pretreatment Protects the Liver From Ischemia-reperfusion Injury Caused By Increasing Hepatic Heat Shock Protein 70. J Surg Res. 2004;122(1):96-102. PubMed PMID: 15522321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70. AU - Bedirli,Abdulkadir, AU - Sakrak,Omer, AU - Muhtaroglu,Sebahattin, AU - Soyuer,Isin, AU - Guler,Ilkay, AU - Riza Erdogan,Ali, AU - Sozuer,Erdogan M, PY - 2004/03/24/received PY - 2004/11/4/pubmed PY - 2004/12/24/medline PY - 2004/11/4/entrez SP - 96 EP - 102 JF - The Journal of surgical research JO - J Surg Res VL - 122 IS - 1 N2 - BACKGROUND: Reperfusion of the liver after ischemia induces the expression of the heat shock genes and the synthesis of the heat shock proteins (HSP). We studied the effects of the natural antioxidant ergothioneine (EGT) treatment on the expression of HSP70 in ischemic-reperfused (IR) liver. METHODS: Adult male Wistar rats were randomly divided into three groups: Sham group given standard laboratory chow and water for 3 weeks followed by sham operation; Control group given standard laboratory chow and water for 3 weeks followed by liver IR injury; EGT group given standard laboratory chow supplementation l-ergothioneine (1.2 mg/kg/d body weight) administered by gavage and water for 3 weeks followed by liver IR injury. Ten rats from each group were killed to determine serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactic dehydrogenase (LDH), tissue malondialdehyde (MDA), HSP70 levels, and histologic changes at 30, 60, and 120 min of reperfusion, respectively. Survival was followed for 1 week. RESULTS: IR caused significant increase in serum AST, ALT, LDH, and tissue MDA levels. As compared with the control group, animals treated with EGT experienced a significant decrease in serum AST, ALT, and LDH levels in all reperfusion periods. Tissue MDA levels in animals receiving EGT were significantly reduced as compared with control group at 30 min and 60 min after reperfusion. After ischemia, reperfusion caused a remarkable production of HSP70 in the control group. When the rats were pretreated with EGT, the levels of HSP70 increased significantly in their livers after reperfusion compared with the control group. Liver injury in the EGT-treated animals was lower to that in the control group. The 7-day survival rate was significantly improved (from 50% to 80%) by EGT pretreatment. CONCLUSION: HSP70 has been shown to induce tolerance against warm IR injury in rat livers. EGT pretreatment protects the liver from IR injury by over-expression of HSP and the subsequent suppression of lipid peroxidation. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/15522321/Ergothioneine_pretreatment_protects_the_liver_from_ischemia_reperfusion_injury_caused_by_increasing_hepatic_heat_shock_protein_70_ DB - PRIME DP - Unbound Medicine ER -