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Cannabinoid CB2 receptor activation inhibits mechanically evoked responses of wide dynamic range dorsal horn neurons in naïve rats and in rat models of inflammatory and neuropathic pain.
Eur J Neurosci. 2004 Nov; 20(9):2311-20.EJ

Abstract

Peripheral cannabinoid 2 receptors (CB2 receptors) modulate immune responses and attenuate nociceptive behaviour in models of acute and persistent pain. The aim of the present study was to investigate whether peripheral CB2 receptors modulate spinal processing of innocuous and noxious responses and to determine whether there are altered roles of CB2 receptors in models of persistent pain. Effects of local administration of the CB2 receptor agonist JWH-133 (5 and 15 microg/50 microL) on mechanically evoked responses of spinal wide dynamic range (WDR) neurons in noninflamed rats, rats with carrageenan-induced hindpaw inflammation, sham operated rats and spinal nerve-ligated (SNL) rats were determined in anaesthetized rats in vivo. Mechanical stimulation (von Frey filaments, 6-80 g) of the peripheral receptive field evoked firing of WDR neurons. Mechanically evoked responses of WDR neurons were similar in noninflamed, carrageenan-inflamed, sham-operated and SNL rats. Intraplantar injection of JWH-133 (15 microg), but not vehicle, significantly (P < 0.05) inhibited innocuous and noxious mechanically evoked responses of WDR neurons in all four groups of rats. In many cases the selective CB2 receptor antagonist, SR144528 (10 microg/50 microL), attenuated the inhibitory effects of JWH-133 (15 microg) on mechanically evoked WDR neuronal responses. The CB1 receptor antagonist, SR141716A, did not attenuate the inhibitory effects of JWH-133 on these responses. Intraplantar preadministration of JWH-133 also inhibited (P < 0.05) carrageenan-induced expansion of peripheral receptive fields of WDR dorsal horn neurons. This study demonstrates that activation of peripheral CB2 receptors attenuates both innocuous- and noxious-evoked responses of WDR neurons in models of acute, inflammatory and neuropathic pain.

Authors+Show Affiliations

Elmes SJ
Institute of Neuroscience, School of Biomedical Sciences, E Floor, Medical School, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
Jhaveri MD
No affiliation info available
Smart D
No affiliation info available
Kendall DA
No affiliation info available
Chapman V
No affiliation info available

MeSH

Action PotentialsAnimalsCamphanesCannabinoidsCarrageenanDisease Models, AnimalInflammationLigationMaleNeural InhibitionNeuralgiaNociceptorsPeripheral Nervous System DiseasesPhysical StimulationPiperidinesPosterior Horn CellsPyrazolesRatsRats, Sprague-DawleyReaction TimeReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2RimonabantSensory Receptor CellsSpinal Nerves

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15525273

Citation

Elmes, Steven J R., et al. "Cannabinoid CB2 Receptor Activation Inhibits Mechanically Evoked Responses of Wide Dynamic Range Dorsal Horn Neurons in Naïve Rats and in Rat Models of Inflammatory and Neuropathic Pain." The European Journal of Neuroscience, vol. 20, no. 9, 2004, pp. 2311-20.
Elmes SJ, Jhaveri MD, Smart D, et al. Cannabinoid CB2 receptor activation inhibits mechanically evoked responses of wide dynamic range dorsal horn neurons in naïve rats and in rat models of inflammatory and neuropathic pain. Eur J Neurosci. 2004;20(9):2311-20.
Elmes, S. J., Jhaveri, M. D., Smart, D., Kendall, D. A., & Chapman, V. (2004). Cannabinoid CB2 receptor activation inhibits mechanically evoked responses of wide dynamic range dorsal horn neurons in naïve rats and in rat models of inflammatory and neuropathic pain. The European Journal of Neuroscience, 20(9), 2311-20.
Elmes SJ, et al. Cannabinoid CB2 Receptor Activation Inhibits Mechanically Evoked Responses of Wide Dynamic Range Dorsal Horn Neurons in Naïve Rats and in Rat Models of Inflammatory and Neuropathic Pain. Eur J Neurosci. 2004;20(9):2311-20. PubMed PMID: 15525273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid CB2 receptor activation inhibits mechanically evoked responses of wide dynamic range dorsal horn neurons in naïve rats and in rat models of inflammatory and neuropathic pain. AU - Elmes,Steven J R, AU - Jhaveri,Maulik D, AU - Smart,Darren, AU - Kendall,David A, AU - Chapman,Victoria, PY - 2004/11/5/pubmed PY - 2005/3/5/medline PY - 2004/11/5/entrez SP - 2311 EP - 20 JF - The European journal of neuroscience JO - Eur J Neurosci VL - 20 IS - 9 N2 - Peripheral cannabinoid 2 receptors (CB2 receptors) modulate immune responses and attenuate nociceptive behaviour in models of acute and persistent pain. The aim of the present study was to investigate whether peripheral CB2 receptors modulate spinal processing of innocuous and noxious responses and to determine whether there are altered roles of CB2 receptors in models of persistent pain. Effects of local administration of the CB2 receptor agonist JWH-133 (5 and 15 microg/50 microL) on mechanically evoked responses of spinal wide dynamic range (WDR) neurons in noninflamed rats, rats with carrageenan-induced hindpaw inflammation, sham operated rats and spinal nerve-ligated (SNL) rats were determined in anaesthetized rats in vivo. Mechanical stimulation (von Frey filaments, 6-80 g) of the peripheral receptive field evoked firing of WDR neurons. Mechanically evoked responses of WDR neurons were similar in noninflamed, carrageenan-inflamed, sham-operated and SNL rats. Intraplantar injection of JWH-133 (15 microg), but not vehicle, significantly (P < 0.05) inhibited innocuous and noxious mechanically evoked responses of WDR neurons in all four groups of rats. In many cases the selective CB2 receptor antagonist, SR144528 (10 microg/50 microL), attenuated the inhibitory effects of JWH-133 (15 microg) on mechanically evoked WDR neuronal responses. The CB1 receptor antagonist, SR141716A, did not attenuate the inhibitory effects of JWH-133 on these responses. Intraplantar preadministration of JWH-133 also inhibited (P < 0.05) carrageenan-induced expansion of peripheral receptive fields of WDR dorsal horn neurons. This study demonstrates that activation of peripheral CB2 receptors attenuates both innocuous- and noxious-evoked responses of WDR neurons in models of acute, inflammatory and neuropathic pain. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/15525273/Cannabinoid_CB2_receptor_activation_inhibits_mechanically_evoked_responses_of_wide_dynamic_range_dorsal_horn_neurons_in_naïve_rats_and_in_rat_models_of_inflammatory_and_neuropathic_pain_ DB - PRIME DP - Unbound Medicine ER -