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Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus.
J Clin Microbiol. 2004 Nov; 42(11):5309-14.JC

Abstract

Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.

Authors+Show Affiliations

Lindsley F. Kimball Research Institute, New York Blood Center, New York, NY 10021, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15528730

Citation

He, Yuxian, et al. "Mapping of Antigenic Sites On the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome Coronavirus." Journal of Clinical Microbiology, vol. 42, no. 11, 2004, pp. 5309-14.
He Y, Zhou Y, Wu H, et al. Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus. J Clin Microbiol. 2004;42(11):5309-14.
He, Y., Zhou, Y., Wu, H., Kou, Z., Liu, S., & Jiang, S. (2004). Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus. Journal of Clinical Microbiology, 42(11), 5309-14.
He Y, et al. Mapping of Antigenic Sites On the Nucleocapsid Protein of the Severe Acute Respiratory Syndrome Coronavirus. J Clin Microbiol. 2004;42(11):5309-14. PubMed PMID: 15528730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus. AU - He,Yuxian, AU - Zhou,Yusen, AU - Wu,Hao, AU - Kou,Zhihua, AU - Liu,Shuwen, AU - Jiang,Shibo, PY - 2004/11/6/pubmed PY - 2005/1/28/medline PY - 2004/11/6/entrez SP - 5309 EP - 14 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 11 N2 - Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15528730/Mapping_of_antigenic_sites_on_the_nucleocapsid_protein_of_the_severe_acute_respiratory_syndrome_coronavirus_ L2 - https://journals.asm.org/doi/10.1128/JCM.42.11.5309-5314.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -